关键词: 溆浦鹅, MSTN, 肌肉组织, 半定量RT-PCR, IGF-I, GH

Abstract: Genome RNA was extracted from leg muscle of Xupu geese,MSTN gene mRNA was amplified by using RT-PCR, the PCR product was cloned into pGEM-T vector.The MSTN gene was isolated and sequenced from the positive clones screened based on the MSTN gene clone, using β-actin as inner control, constructing an optimal semi-quantitative RT-PCR method, the difference of MSTN gene expression at 21 and 70 days by feeding different energy diets, at one time we detected IGF-I and GH in serum. The unique DNA fragment about 1 128 bp in length was obtained. Sequence analysis revealed that it has a length of 1 128 bp which encodes a 375 amino peptide. Compared with homologous sequence of chicken, duck and Landes geese, it displayed a fairly high conservation. The homologue of the nucleotide sequence are 94%,94%,99%, respectively; the homologue of the amino acid sequence deduced are 98%, 97%,98%, respectively. The results showed the expressions of MSTN in Xupu geese were showed no significantly differences at 21 days; but the expression of MSTN was C>B>A by 70 days; During 2170 days period, the expression of MSTN was changed with IGF-I in serum; it was not associated with GH in serum. The different energy influenced MSTN gene expression of Xupu geese after 21 days. The present study laid a foundation for further investigating MSTN gene in waterfowl.

Key words: Xupu geese, MSTN, muscle tissue, semi-quantitative RT-PCR, IGF-I, GH