EGF和IGF-I对绵羊卵母细胞体外成熟和卵裂的影响

畜牧兽医学报 ›› 2008, Vol. 39 ›› Issue (5): 588-593.

EGF和IGF-I对绵羊卵母细胞体外成熟和卵裂的影响

刘丑生¹，陆会宁²，张利平²，王志刚¹，赵俊金¹ ，孟飞¹

1.全国畜牧兽医总站畜禽牧草种质资源保存利用中心，北京 100094；2.甘肃农业大学动物科学技术学院，兰州 730070

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-05-24 发布日期:2008-05-24

Effects of EGF，IGF-I on in vitro Maturation and Cleavage of Ovine Oocytes

LIU Chou-sheng ¹，LU Hui-ning ²，ZHANG Li-ping ² ，
WANG Zhi-gang ¹， ZHAO Jun-jin ¹ ，MENG Fei ¹

1. The Center of Preservation and Utilization of Germplasm Resources of Domestic Animals and Forage, National Animal Husbandry & Veterinary Service, Beijing 100094,China；2.College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070,China

Received:1900-01-01 Revised:1900-01-01 Online:2008-05-24 Published:2008-05-24

摘要/Abstract

摘要： 本文研究了EGF、IGF-I以及EGF和IGF-I联合对绵羊卵母细胞体外成熟和卵裂的影响，以确立绵羊卵母细胞体外成熟的最佳条件。结果表明:50 ng/mL的EGF的成熟率和卵裂率分别为71.2%和45.5%，显著高于对照组和10、20、30、40 ng/mL组(P<0.05) ，当EGF浓度达到100 ng/mL时，成熟率和卵裂率最高，分别为72.9%和45.7%；40 ng/mL IGF-1的成熟率和卵裂率分别为70.7%和58.5%，显著高于对照组和10、20、60、80、100 ng/mL组(P <0.05) ,当IGF-I的浓度为100 ng/mL时，成熟率和卵裂率最低，分别为38.8%和20.0%，与对照组和其它各试验组差异显著(P <0.05)；50 ng/mL EGF和 40 ng/mL IGF-I联合使用，成熟率和卵裂率分别为85.6%和61.0%，同对照组和50 ng/mL EGF组、40 ng/mL IGF-I组之间差异显著(P <0.05)。

关键词: 绵羊, 卵母细胞, 体外成熟, 卵裂, 表皮生长因子, 胰岛素生长因子-I

Abstract: To establish an optimal conditions for in vitro maturation of ovine oocytes, we studied the effects of EGF, IGF-I and the union use of both of them on in vitro maturation and cleavage of ovine oocytes. The results showed that the maturation and cleavage rates derived from oocytes matured in medium supplemented with 50 ng/mL EGF alone were 71.2% and 45.5%， respectively, which was significantly higher than those in control and those with 10，20，30，40 ng/mL EGF, and the highest maturation and cleavage rates present in medium with 100 ng/mL EGF, were 70.7% and 58.5%, respectively. While the oocytes were only treated with 40 ng/mL IGFI, the maturation and cleavage rates were 70.7% and 58.5%, respectively, which was noticeably higher than that of the control and those with 10，20， 60，80，100 ng/mL IGF-I, but the maturation and cleavage rates were only 38.8% and 20.0%, respectively when the concentration of IGF-I was 100 ng/mL, the difference is remarkable compared to the experimental groups and control group. When treated by the combination of 50 ng/mL EGF and 40 ng/mL IGF-I, the maturation and cleavage rates were 85.6% and 61.0%, respectively, which is significantly higher than the single use of 50 ng/mL EGF or 40 ng/mL IGF-I and the control group.

Key words: ovine, oocytes, IVM, cleavage, EFG, IGF-I

刘丑生;陆会宁;张利平;王志刚;赵俊金;孟飞. EGF和IGF-I对绵羊卵母细胞体外成熟和卵裂的影响[J]. 畜牧兽医学报, 2008, 39(5): 588-593.

LIU Chou-sheng;LU Hui-ning;ZHANG Li-ping ;WANG Zhi-gang;ZHAO Jun-jin ;MENG Fei . Effects of EGF，IGF-I on in vitro Maturation and Cleavage of Ovine Oocytes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2008, 39(5): 588-593.

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