奶牛围产期血清脂肪代谢、肝脏功能和氧化指标的变化

畜牧兽医学报 ›› 2010, Vol. 41 ›› Issue (8): 1039-1045.

奶牛围产期血清脂肪代谢、肝脏功能和氧化指标的变化

熊桂林¹，付志新^1,2，曹随忠^1,3，顾建红¹，陈大伟¹，刘宗平¹*

1. 扬州大学兽医学院，扬州 225009；2. 河北科技师范学院，秦皇岛 066600；3. 四川农业大学动物医学院，雅安 625014

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-08-20 发布日期:2010-08-20
通讯作者: 刘宗平

Changes in Serum Fat Metabolism, Liver Function and Lipid Peroxidation of the Transition Cow

XIONG Gui-lin¹, FU Zhi-xin^1,2, CAO Sui-zhong^1,3, GU Jian-hong¹,CHEN Da-wei¹, LIU Zong-ping¹*

1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;2. Hebei Science & Technology of Normal University, Qinhuangdao 066600, China;3. College of Veterinary Medicine, Sichuan Agricultural University, Ya′an 625014, China

Received:1900-01-01 Revised:1900-01-01 Online:2010-08-20 Published:2010-08-20

摘要/Abstract

摘要： 选择产前30 d的奶牛30头，分别在产前30、20、10、5、0 d和产后5、10、15、20 d采集血清，检测脂肪代谢指标（TG、Chol、NEFA、HDL、LDL含量）、肝脏功能指标（AKP、LDH、ALT、AST活性）和抗氧化、脂质过氧化指标（GSHPx、SOD、CAT活性和MDA含量）。结果表明：①血清TG和Chol含量产前呈降低趋势，TG含量产犊当天急剧下降，Chol含量产后逐渐升高；血清NEFA含量产前变化不明显，产犊当天急剧升高，产犊5 d开始降低；血清LDL和HDL水平产前呈降低趋势，产后逐渐升高。②血清LDH和AST活性在产前5 d或产犊当天开始升高，产犊5 d后逐渐降低；ALT和AKP活性各时间段变化不明显。③血清CAT和GSH-Px活性在产前呈降低的趋势，产犊当天升高，之后逐渐降低；血清SOD活性产前呈升高趋势，产犊当天达到最高峰，之后逐渐降低；血清MDA含量在产前5 d开始升高，产犊5 d后逐渐降低。表明奶牛围产期发生脂肪动员和氧化状态失衡。

关键词: 奶牛, 围产期, 肝脏功能, 脂肪代谢, 氧化状态

Abstract: Thirty Holstein dairy cows at 30 d before calving were selected for this study. Serum samples were collected at 30, 20, 10, 5 d before calving, 0 d calving and 5, 10, 15, 20 d after calving. Fat metabolism (concentrations of TG, Chol, NEFA, HDL and LDL), liver enzyme activities (AKP, LDH, ALT and AST activities), and antioxidation, lipid peroxidation (GSH-Px, SOD and CAT activities, MDA content) indexes in serum were measured. The result showed that: ① Concentrations of serum TG had a declining trend from 30 d before calving and decreased sharply on the day of calving. Concentration of Chol had a declining trend before calving and then increasing after calving. NEFA concentrations increased at calving, reaching peak level on day 5 after calving and started to decrease thereafter. Concentrations of serum HDL and LDL decreased slightly before calving period and increased gradually after calving. ② LDH and AST activities were the highest at day 5 before calving and the day of calving, respectively. ALT and AKP activities did not differ significantly at any stage. ③ CAT and GSH-Px activities showed a declining trend before calving period and increased at the day of calving followed by a decrease. SOD activity had increasing trend from day -30 to 0 and started to decrease thereafter. MDA content increased from day 5 before calving and decreased from day 5 after calving. In conclusion, there were the extensive mobilization of body fat and an imbalance in the oxidative status in the transition period of dairy cows.

Key words: dairy cows, transition period, liver function, fat metabolism, oxidative status

熊桂林;付志新;曹随忠;顾建红;陈大伟;刘宗平. 奶牛围产期血清脂肪代谢、肝脏功能和氧化指标的变化[J]. 畜牧兽医学报, 2010, 41(8): 1039-1045.

XIONG Gui-lin;FU Zhi-xin;CAO Sui-zhong;GU Jian-hong;CHEN Da-wei;LIU Zong-ping. Changes in Serum Fat Metabolism, Liver Function and Lipid Peroxidation of the Transition Cow[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(8): 1039-1045.

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