关键词: 单色光, 蛋鸡, 脾脏, 增殖细胞核抗原

Abstract: The effects of monochromatic light on spleen histology and proliferation response of splenocyte in laying hens of laying period were investigated by histological and immunohistochemical methods. The results showed that under incandescent light, the diameters of spleen node among various age laying hens were no significant difference (P＞005), but area of periaterial lymphatic shealth of the 37 weeks laying hens were decreased significantly (P＜005) by 1334% compared with 29 weeks. Blue light highly significantly (P＜001) increased the diameter of spleen node by 2094% and 2027% compared with white light, at 29 weeks and 37 weeks old. Blue light highly significantly (P＜001) increased area of periaterial lymphatic shealth by 4201% and 3646% compared with red light, at 29 weeks and 37 weeks old. Furthermore, the 29 weeks and 37 weeks laying hens reared under blue light both had longer diameters of spleen node than the 20 weeks and 52 weeks laying hens reared under blue light, area of periaterial lymphatic shealth of the 29 weeks laying hens were the biggest in all groups under blue light. The diameter of spleen node of the red light group were increased with age from 20 weeks to 37 weeks; area of periaterial lymphatic shealth decreased at 37 weeks old, but rebounded at 52 weeks old. The percentages of proliferating cell nuclear antigen ( PCNA ) immunopositive cells accounting for the total spleen cells of all light groups decreased highly significantly (P＜001) with age. Compared with white light, blue light significantly (P＜005) increased the percentages of PCNA immunopositive cells accounting for spleen cells by 1147% and 1155%, respectively, at both 29 and 37 weeks old. The results suggested that under incandescent light, the splenocyte immune function and splenocyte proliferation of laying hens in laying period decreased with age. The blue light increased laying hens splenocyte immune function and splenocyte proliferation at peak period (29 weeks—35 weeks), while splenocyte immune function would be enhanced in laying hens reared under red light in latter stage of hens laying period.

Key words: monochromatic light, laying hens, spleen, proliferating cell nuclear antigen