硒对氟中毒雏鸡肝脏细胞色素P450酶系主要亚型的影响

畜牧兽医学报 ›› 2009, Vol. 40 ›› Issue (6): 922-927.

硒对氟中毒雏鸡肝脏细胞色素P450酶系主要亚型的影响

于倩，左楠，贾海燕，李金敏，王俊杰，李术*

东北农业大学动物医学学院，哈尔滨 150030

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-06-26 发布日期:2009-06-26
通讯作者: 李术

The Effect of Selenium on Main Subtypes of Hepatic Microsomalcytochrome P450s in Chickens with Exposure of Fluoride

YU Qian, ZUO Nan, JIA Haiyan, LI Jinmin, WANG Junjie, LI Shu

College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China

Received:1900-01-01 Revised:1900-01-01 Online:2009-06-26 Published:2009-06-26
Contact: LI Shu

摘要/Abstract

摘要： 本试验旨在研究硒对氟中毒雏鸡肝脏细胞色素P450酶系主要亚型活性（含量）的影响及CYP3A37基因转录情况。选取180羽7日龄健康雏鸡，随机分为3组，分别为正常组、氟中毒组和加硒组。正常组饲喂全价日粮；氟中毒组在正常日粮中添加氟化钠（NaF），使日粮中氟含量为1 000 mg·kg-1；加硒组在氟中毒组日粮基础上添加亚硒酸钠（Na2SeO3），使日粮中硒含量为4 mg·kg-1。分别在第30、60、90天从各组随机选取20羽鸡，采用比色法测定P450酶系主要亚型活性（含量），利用RTPCR方法测定CYP3A37 mRNA转录水平的变化。在添加氟化钠后第30天，氟中毒组除细胞色素P450含量和氨基比林N脱甲基酶（AND）活性低于正常组外，其余各酶活性（含量）较正常组均有增加（P<005）；在加硒组中，除NADPH细胞色素C还原酶和苯胺4羟化酶活性低于氟中毒组外，其余各亚型酶活性（含量）均高于氟中毒组（P<005）。第60天时，氟中毒组酶活性（含量）均极显著高于正常组（P<001）；在加硒组中，除NADPH细胞色素C还原酶和氨基比林N脱甲基酶活性略高于氟中毒组外（P>005），其余各亚型酶活性（含量）均极显著低于氟中毒组（P<001）。第90天时，氟中毒组除细胞色素P450含量略高于正常组外（P>005），其余各亚型酶活性（含量）均极显著低于正常组（P<001）；加硒组中细胞色素P450含量和NADPH细胞色素C还原酶活性略低于氟中毒组，其余各亚型酶活性（含量）均高于氟中毒组。在各时间点氟中毒组CYP3A37 mRNA转录水平均明显高于正常组（P<001）；加硒组中mRNA转录水平介于氟中毒组和正常组之间。结果提示，饲料中添加氟化钠和亚硒酸钠可使鸡肝脏细胞色素P450酶系各亚型活性（含量）及CYP3A37 mRNA转录水平发生明显变化。

关键词: 细胞色素P450, 鸡, 硒, 氟

Abstract: The antagonism effect of selenium to fluorine on main subtypes of hepatic microsomalcytochrome P450s and the CYP3A37 mRNA transcription level in chickens were investigated. 180 7dayold healthy chickens were divided into 3 groups at random. The first group were fed with adequate ration (normal control group).The second group were fed with 1 000 mg·kg-1 diet NaF adequate ration (fluorosis group).The third group were fed with 1 000 mg·kg-1 diet NaF and 4 mg·kg-1 diet Na2SeO3 adequate ration (selenium group).The whole experiment lasted for 90 days. On 30th, 60th, 90th day, 20 chickens in each group were randomly selected for delecting hepatic microsomalcytochrome P450s activities and CYP3A37 transcription level. Results: On the 30th day, the hepatic microsomalcytochrome P450s activities of fluorosis group were higher than that of normal group (P<005), except the CYP450 contents and the AND activities. In selenium group, except NADPHCytC activities and AH activities were lower than that of fluorosis group, the other enzymes′ activities were higher（P<005）.On the 60th day, all the fluorosis group′s hepatic microsomalcytochrome P450s activities were significantly higher than that of normal group (P<001).In selenium group, the activities of NADPHCytC and AND were higher than that of fluorosis group(P＞005),and the other enzymes′ activities were significantly lower (P＜001). On the 90th day, all the fluorosis group′s hepatic microsomalcytochrome P450s activities were significantly lower than that of normal group (P<001) except CYP450. In selenium group, CYP450 and NADPHCytC activities were slightly lower than that of fluorosis group,and the other enzymes′ activities were higher. During each stage,transcription level of CYP3A37 mRNA in fluorosis group was higher than that of normal group′s (P<001). The mRNA transcription level in selenium group lies between that of normal group and fluorosis group. Fluoride can make significantly changes on the activities of microsomalcytochrome P450s and the transcription level of CYP3A37 mRNA. These results indicated that microsomalcytochrome P450s participated in the process which selenium antagonized the toxicity of liver induced by fluoride.

Key words: cytochrome P450s, chicken, selenium, fluoride

于倩;左楠;贾海燕;李金敏;王俊杰;李术. 硒对氟中毒雏鸡肝脏细胞色素P450酶系主要亚型的影响[J]. 畜牧兽医学报, 2009, 40(6): 922-927.

YU Qian;ZUO Nan;JIA Haiyan;LI Jinmin;WANG Junjie;LI Shu. The Effect of Selenium on Main Subtypes of Hepatic Microsomalcytochrome P450s in Chickens with Exposure of Fluoride[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(6): 922-927.

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