桦褐孔菌多糖对弓形虫感染小鼠基因表达谱的影响

畜牧兽医学报 ›› 2012, Vol. 43 ›› Issue (7): 1134-1142.

桦褐孔菌多糖对弓形虫感染小鼠基因表达谱的影响

李佳佳1，鞠玉琳2*，张雪梅2，李杨3，孙福亮2

1. 上海柯莱逊生物技术有限公司，上海 201201； 2. 延边大学农学院动物医学系，延吉 133400；3. 长春普莱医药生物技术有限公司，长春 130012

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-07-27 发布日期:2012-07-27
通讯作者: 鞠玉琳

The Effects of Polysaccharides in Inonotus obliquus on Differential Gene Expression Profiling in Mouse Infected with Toxoplasma gondii

LI Jiajia1, JU Yulin2*, ZHANG Xuemei2, LI Yang3, SUN Fuliang2

1. Shanghai Claison Biotech, Co., Ltd, Shanghai 201201, China; 2. The Agricultural College of Yanbian University, Yanji 133400, China; 3. Changchun ProteLight Pharmaceutical ＆ Biotechnology Co. Ltd, Changchun 130012, China

Received:1900-01-01 Revised:1900-01-01 Online:2012-07-27 Published:2012-07-27
Contact: JU Yulin

摘要/Abstract

摘要：

探讨桦褐孔菌多糖对感染弓形虫小鼠基因表达谱的影响，并对其抗虫机理进行分析。BABL/C小鼠腹腔接种RH株弓形虫速殖子103个，建立小鼠弓形虫感染模型。将小鼠随机分成3组：空白对照组、感染对照组、桦褐孔菌多糖治疗组。采集动物肝脏迅速冻存，提取总RNA，检测RNA质量，扩增RNA,进行芯片杂交，扫描后进行数据分析。结果：经SOM分析筛选出桦褐孔菌多糖治疗组的有效差异基因，并对其进行功能注释，分析其治疗弓形虫的主要原因。结果表明：桦褐孔菌多糖可有效治疗弓形虫感染引起的病理损伤，其抗弓形虫机理是通过调节宿主细胞因子水平，促进Th1/Th2平衡，控制Toll样受体信号通路以及对机体整体调控达到抗弓形虫目的。

关键词: 桦褐孔菌多糖, 弓形虫, 基因表达谱, 抗虫机理

Abstract:

The objective of this study was to observe the effects of polysaccharides in Inonotus obliquus on differential gene expression profiling in mouse infected with Toxoplasma gondii and discuss the mechanism of inhibiting Toxoplasma gondii. BABL/C mice were inoculated intraperitoneally with 103 tachyzoites of Toxoplasma gondii of RH strain to establish a mouse model of infection of toxoplasmosis. The mice were randomly divided into three groups: normal control group, negative control group, Inonotus obliquus polysaccharide group. Animal liver were collected and frozenstored quickly. Its RNA was extracted and amplified, then hybridization was conducted. The figures were scanned for data analysis. The results were as follows: The effective differential genes of polysaccharide group were searched out by SOM analysis. The effective differential genes were annotated on gene functional aspect. The main reasons of which Inonotus obliquus polysaccharide inhibited Toxoplasma were announced. The mechanism that the effects of polysaccharides in Inonotus obliquus inhibited Toxoplasma gondii are achieved by effectively regulating the cytokine levels of mice, promoting Th1/Th2 balance, controlling Tolllike receptor signaling pathways and integral controlling the body.

Key words: polysaccharides in Inonotus obliquus, Toxoplasma gondii, gene expression profiling, parasite resistance mechanism

李佳佳;鞠玉琳;张雪梅;李杨;孙福亮. 桦褐孔菌多糖对弓形虫感染小鼠基因表达谱的影响[J]. 畜牧兽医学报, 2012, 43(7): 1134-1142.

LI Jiajia;JU Yulin;ZHANG Xuemei;LI Yang;SUN Fuliang. The Effects of Polysaccharides in Inonotus obliquus on Differential Gene Expression Profiling in Mouse Infected with Toxoplasma gondii[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(7): 1134-1142.

[1]	高俊莹, 张东超, 李璇, 王华琳, 姜宁. 弓形虫表面抗原SAG1黏附宿主细胞表面硫化肝素的特性研究[J]. 畜牧兽医学报, 2019, 50(9): 1874-1881.
[2]	李丽敏, 邹云婧, 郝雪飘, 赵兴华, 李睿文, 孙继国, 袁万哲. 脑心肌炎病毒感染神经细胞的基因表达谱分析[J]. 畜牧兽医学报, 2019, 50(8): 1709-1714.
[3]	李丽敏, 邹云婧, 郝雪飘, 赵兴华, 李睿文, 孙继国, 袁万哲. 脑心肌炎病毒感染神经细胞的基因表达谱分析[J]. 畜牧兽医学报, 2019, 50(8): 1709-1714.
[4]	马群山, 刘欣超, 孙晓妮, 王帅, 徐立新, 宋小凯, 严若峰, 李祥瑞. 刚地弓形虫钙依赖蛋白激酶9对小鼠Ana-1巨噬细胞功能的影响[J]. 畜牧兽医学报, 2017, 48(4): 731-739.
[5]	马群山, 刘欣超, 孙晓妮, 王帅, 徐立新, 宋小凯, 严若峰, 李祥瑞. 刚地弓形虫钙依赖蛋白激酶9对小鼠Ana-1巨噬细胞功能的影响[J]. 畜牧兽医学报, 2017, 48(4): 731-739.
[6]	李祎, 李星, 周冰洁, 李木子, 马磊, 刘群, 刘晶. 三株弓形虫对昆明鼠致病性的比较研究[J]. 畜牧兽医学报, 2017, 48(10): 1976-1982.
[7]	李祎, 李星, 周冰洁, 李木子, 马磊, 刘群, 刘晶. 三株弓形虫对昆明鼠致病性的比较研究[J]. 畜牧兽医学报, 2017, 48(10): 1976-1982.
[8]	富丽，孙研研，薛夫光，刘冉冉，白皓，常国斌，陈国宏，陈继兰. 公鸡弱精症相关候选基因的表达分析[J]. 畜牧兽医学报, 2015, 46(6): 889-895.
[9]	富丽，孙研研，薛夫光，刘冉冉，白皓，常国斌，陈国宏，陈继兰. 公鸡弱精症相关候选基因的表达分析[J]. 畜牧兽医学报, 2015, 46(6): 889-895.
[10]	赵东岳，陈金锋，韦剑辉. 弓形虫截短SAG2基因的克隆表达与免疫活性分析[J]. 畜牧兽医学报, 2015, 46(12): 2258-2263.
[11]	赵东岳，陈金锋，韦剑辉. 弓形虫截短SAG2基因的克隆表达与免疫活性分析[J]. 畜牧兽医学报, 2015, 46(12): 2258-2263.
[12]	叶强，王萌，张念章，殷宏，张德林. 弓形虫强免疫原性排泄分泌抗原组分的鉴定[J]. 畜牧兽医学报, 2015, 46(11): 2063-2068.
[13]	叶强，王萌，张念章，殷宏，张德林. 弓形虫强免疫原性排泄分泌抗原组分的鉴定[J]. 畜牧兽医学报, 2015, 46(11): 2063-2068.
[14]	杭柏林，桑建君，钱琨，叶建强，邵红霞，金文杰，梅梅，缪佶，秦爱建. 利用基因芯片分析鸡法氏囊差异表达基因[J]. 畜牧兽医学报, 2014, 45(7): 1162-1169.
[15]	杭柏林，桑建君，钱琨，叶建强，邵红霞，金文杰，梅梅，缪佶，秦爱建. 利用基因芯片分析鸡法氏囊差异表达基因[J]. 畜牧兽医学报, 2014, 45(7): 1162-1169.