Abstract:

The objective of this study was to observe the effects of polysaccharides in Inonotus obliquus on differential gene expression profiling in mouse infected with Toxoplasma gondii and discuss the mechanism of inhibiting Toxoplasma gondii. BABL/C mice were inoculated intraperitoneally with 103 tachyzoites of Toxoplasma gondii of RH strain to establish a mouse model of infection of toxoplasmosis. The mice were randomly divided into three groups: normal control group, negative control group, Inonotus obliquus polysaccharide group. Animal liver were collected and frozenstored quickly. Its RNA was extracted and amplified, then hybridization was conducted. The figures were scanned for data analysis. The results were as follows: The effective differential genes of polysaccharide group were searched out by SOM analysis. The effective differential genes were annotated on gene functional aspect. The main reasons of which Inonotus obliquus polysaccharide inhibited Toxoplasma were announced. The mechanism that the effects of polysaccharides in Inonotus obliquus inhibited Toxoplasma gondii are achieved by effectively regulating the cytokine levels of mice, promoting Th1/Th2 balance, controlling Tolllike receptor signaling pathways and integral controlling the body.