十二指肠大豆小肽梯度灌注对山羊乳腺氨基酸吸收与氨肽酶N基因表达的影响

畜牧兽医学报 ›› 2009, Vol. 40 ›› Issue (12): 1761-1768.

十二指肠大豆小肽梯度灌注对山羊乳腺氨基酸吸收与氨肽酶N基因表达的影响

刘辉^1,2，3，王玲^1,2，4，李胜利^1,2*，王立斌^1,2

1.中国农业大学中美奶牛研究中心，北京100193；2. 动物营养学国家重点实验室，北京 100193；3.北京首都农业集团三元绿荷奶牛养殖中心，北京100076；4. 宁夏大学农学院，银川 750021

收稿日期:2008-11-28 修回日期:1900-01-01 出版日期:2009-12-24 发布日期:2009-12-24
通讯作者: 李胜利

Effect of Duodenal Soybean Small Peptides Infusion on Mammary Uptake of Amino Acid and Expression of APN in Lactating Goat

LIU Hui ^1,2,3, WANG Ling^1,2,4, LI Shengli ^1,2*, WANG Libin^1,2

1.SinoUS Dairy Research & Development Center, China Agricultural University,Beijing 100193, China； 2.State Key Laboratory of Animal Nutrition, Beijing 100193,China；3.Sanyuan Lvhe Dairy Cattle Center, Capital Agribusiness Group, Beijing 100076, China; 4. College of Agriculture, Ningxia University, Yinchuan 750021, China

Received:2008-11-28 Revised:1900-01-01 Online:2009-12-24 Published:2009-12-24
Contact: LI Shengli

摘要/Abstract

摘要： 旨在研究乳腺对不同水平大豆小肽的吸收利用情况。试验选用8只带有十二指肠瘘管和颈动脉、乳静脉血插管的泌乳奶山羊（体质量38 kg±2 kg），采用交叉设计，分别向十二指肠灌注大豆小肽0、60、120和180 g·d1,连续灌注14 d。结果表明：通过小肽的灌注，（1）提高了乳蛋白产量与乳蛋白含量，而且乳蛋白产量显著提高（P<0.05）。乳脂产量与乳脂含量随着灌注量的升高呈显著下降趋势（P<0.05）。（2）乳腺血浆流量有轻微的提高，但不显著（P>0.05）。与对照组相比，各处理组血浆流量/产奶量出现下降，但只有120 g·d1灌注组下降显著（P<0.05）。（3）与对照组相比，大部分游离氨基酸在60和120 g·d1灌注组吸收量增加，而在180 g·d1灌注组出现下降趋势。除了PBIle，大豆小肽的灌注增加了所有肽结合必需氨基酸的乳腺吸收。但PBVal、 PBLeu、PBPhe、PBThr、PBMet和 PBLys均在120 g·d1灌注组吸收率最高。非必需氨基酸中，小肽的灌注提高了PBSer、PBTyr、PBPro 的吸收(P<0.05)，而PBGly的吸收却出现了下降 (P>0.05)。（4）除了Lys，处理组中所有必需氨基酸的乳中分泌量都高于对照组，从0~120 g·d1试验组，这些氨基酸乳中分泌量随着小肽灌注量的升高而呈增加趋势（P<0.05）；在180 g·d1灌注组，大部分必需氨基酸的分泌量增加不显著（P>0.05），且低于120 g·d1灌注组。（5）小肽的灌注明显促进了APN基因的表达。灌注60、120和180 g·d1小肽后，氨肽酶N（APN）的表达分别是对照组的13.55、18.69和10.01倍。结论：乳腺组织能够吸收动脉血液中的小肽，大豆小肽的灌注提高了乳蛋白产量显示乳腺组织可以将吸收的小肽用于乳蛋白的合成。但当乳腺所需的氨基酸达到饱和以后，再增加氨基酸的浓度会出现吸收抑制。APN表达变化与乳腺小肽吸收增加相一致，这可能因为APN是调控小肽乳腺吸收利用的主要酶类之一。

关键词: 大豆小肽, 乳腺, 氨基酸, 吸收, APN

Abstract: The aim of this study was to investigate effect of duodenal soybean small peptides (SSP) infusion on mammary uptake of amino acids (AA). Eight Chinese Saanen goats with duodenal fistula and catheter were used in a crossover design trial. Goats were infused 0, 60, 120, 180 g·d1SSP. The experiment period lasted for 14 d. The results showed: 1) milk protein yield and content were increased, increment of milk protein yield were significant (P<0.05). Milk fat yield and content were decreased with the increased of the amount of SSP infusion (P<0.05). 2) Mammary plasma flow were no changed by SSP infusion though the average was slightly higher (P>0.05). Mammary plasma flow/milk yield was decreased by SSP infusion and there was significant difference between the 120 g·d1treatment and the control (P<0.05). 3) Compared with the control treatment, uptakes of most free amino acids were increased in the 60 and 120 g·d1 treatments, and decreased in 180 g·d1treatment. The uptakes of all peptidebound essential amino acids (PBEAA) were increased except for PBIle. Uptake of PBVal, PBLeu, PBPhe, PBThr, PBMet and PBLys was the highest in the 120 g·d1 treatment. Among peptidebound nonessential amino acids (PBNEAA), uptake of PBSer, PBTyr, PBPro was increased (P<0.05). However, that of PBGly was drop down (P>005). 4) Secretion of all essential AA (EAA) was increased by SSP infusion apart from Lys, that of those EAA was increased with increment of the amount of SSP infusion from 0102 g·d1 treatment (P<0.05), however increment of their secretion in milk were not significant in the 180 g·d1 treatment and were lower than in the 120 g·d1 treatment. 5) The expression of APN was improved by SSP, that in 60, 120, 180 g·d1 treatment was 13.55, 18.69, 10.01fold in control treatment. Conclusion: SSP might be used as substrate of milk protein synthesis by mammary gland, and improved milk protein synthesis and secretion. However, when the concentration of AA was stature, increment of AA resulted in absorption inhibition. Increment of APN expression accorded with the variety of PBAA uptakes by mammary gland, which suggested APN was one of peptidase regulated small peptides absorption in mammary tissue.

Key words: soybean small peptides, mammary gland, amino acid, uptake, APN

刘辉;;王玲;;李胜利;王立斌;. 十二指肠大豆小肽梯度灌注对山羊乳腺氨基酸吸收与氨肽酶N基因表达的影响[J]. 畜牧兽医学报, 2009, 40(12): 1761-1768.

LIU Hui;;WANG Ling;;LI Shengli;WANG Libin;. Effect of Duodenal Soybean Small Peptides Infusion on Mammary Uptake of Amino Acid and Expression of APN in Lactating Goat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(12): 1761-1768.

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