应用15N和嘌呤估测肉羊微生物N产量的研究

畜牧兽医学报

应用¹⁵N和嘌呤估测肉羊微生物N产量的研究

马涛¹，刁其玉^1*，邓凯东²，姜成钢¹，屠焰¹，张乃锋¹，王永超¹，刘洁¹，赵一广¹

(1. 中国农业科学院饲料研究所农业部饲料生物技术重点实验室，北京 100081；2. 金陵科技学院动物科学与技术学院，南京 210038）

收稿日期:2012-02-05 出版日期:2012-12-26 发布日期:2012-12-26
通讯作者: 刁其玉，教授，博导，E-mail：diaoqiyu@mail.caas.net.cn
作者简介:马涛（1987-），男，山东青岛人，博士生，主要从事动物营养与饲料方面研究，E-mail：nemesisematao@yahoo.com.cn
基金资助:
国家现代农业肉羊产业技术体系专项资金（CARS-39）；公益性行业科研专项“饲料营养价值与畜牧饲养标准研究与应用”（200903006-03）

Study on Microbial N Yield Measured with ¹⁵N and Purine Bases in Mutton Sheep

MA Tao¹, DIAO Qi-yu^1*, DENG Kai-dong², JIANG Cheng-gang¹, TU Yan¹, ZHANG Nai-feng¹, WANG Yong-chao¹, LIU Jie¹, ZHAO Yi-guang¹

(1. Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2. College of Animal Science and Technology, Jinling Institute of Technology, Nanjing 210038, China)

Received:2012-02-05 Online:2012-12-26 Published:2012-12-26

摘要/Abstract

摘要：

本试验通过比较2种微生物标记物¹⁵N和嘌呤（Purine bases, PB）估测肉羊瘤胃微生物N产量的效果，旨在建立不同方法之间的内在联系，为准确地估测肉羊微生物N提供依据。试验选用12只平均体质量为(41.3±2.8) kg的杜寒杂交(杜泊羊×小尾寒羊杂交)绵羊公羔，随机均分为3组，按照自由采食、自由采食量的70%和40%3个干物质采食水平饲喂，试验共持续25 d，其中预试期10 d，正试期15 d，最后5 d采集瘤胃食糜和十二指肠食糜并测定¹⁵N和PB。结果表明,瘤胃细菌成分（氮含量、嘌呤含量和嘌呤/氮）不受日粮处理的影响 (P>0.05)；十二指肠干物质、有机物、非氨氮和嘌呤流量均随日粮饲喂水平的降低显著下降（P<0.05）；应用¹⁵N和PB计算得到的微生物N产量均随日粮饲喂水平的降低而显著降低（P<0.05），计算得出的微生物合成效率则均不受日粮处理的显著影响（P>0.05)。¹⁵N测定的微生物N在各处理内的变异性要小于PB测定得到的微生物N，因此应用¹⁵N来测定微生物N能够得到更为准确可靠的结果。

Abstract:

Two different microbial markers, ¹⁵N and purine bases(PB), were used in this experiment with the purpose to establish the relationship between the two methods and provide a precise way for measuring microbial N yield in mutton sheep. Twelve Dorper ( Small-tailed Han crossbreds, noncastrated male lambs ((41.3±2.8) kg BW) were randomly assigned to three levels of dry matter intake: ad libitum intake, 70% or 40% of the ad libitum intake, with four lambs at each level. The experiment lasted for 25 d (10 d for adaptation and 15 d for trial period). The ruminal bacterial components (N, purine bases and purine bases/N) were not affected by dietary treatment (P>0.05). Duodenal flows of dry matter, organic matter, non-ammonia nitrogen and PB decreased significantly with decrease of feed intake (P<0.05). Microbial N yield measured with either ¹⁵N or PB decreased significantly with decrease of feed intake (P<0.05) whereas the efficiency of microbial synthesis was not affected by dietary treatment for both markers (P>0.05). The result indicate that microbial N measured with ¹⁵N showed a smaller within-treatment variation than that measured with PB, and thus ¹⁵N can be a reliable marker for accurately quantifying microbial N.

中图分类号:

马涛，刁其玉，邓凯东，姜成钢，屠焰，张乃锋，王永超，刘洁，赵一广. 应用¹⁵N和嘌呤估测肉羊微生物N产量的研究[J]. 畜牧兽医学报, doi: .

MA Tao, DIAO Qi-yu, DENG Kai-dong, JIANG Cheng-gang, TU Yan, ZHANG Nai-feng, WANG Yong-chao, LIU Jie, ZHAO Yi-guang. Study on Microbial N Yield Measured with ¹⁵N and Purine Bases in Mutton Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/

https://www.xmsyxb.com/CN/Y2012/V43/I12/1910

参考文献

［1］GALVANI D B, PIRES C C, KOZLOSKI G V, et al. Protein requirements of Texel crossbred lambs［J］. Small Rumin Res，2009，81：55-62.

［2］SUBBA D B. Purine nitrogen index, a possible parameter for rapid feed evaluation in ruminants［D］. A thesis for the degree in M. Sc. In animal nutrition. University of Aberdeen，1997：12.

［3］MCDONALD P, EDWARDS R A, GREENHALGH, et al. Animal nutrition［M］. Fifth edition. Longman scientific and technical, New York, 1995.

［4］PUCHALA R, KULASEK G. Estimation of microbial protein flow from the rumen of sheep using microbial nucleic acid and urinary excretion of purine derivatives［J］. Can J Anim Sci，1992，72：821-830.

［5］马涛，刁其玉，邓凯东. 尿嘌呤衍生物法估测瘤胃微生物蛋白质产量的研究进展［J］. 动物营养学报，2011，23（1）：10-14.

［6］UDEN P, COLUCCI P E, VAN SOEST P J. Investigation of chromium, cerium and cobalts as markers in digesta: Rate of passage studies［J］. J Sci Food Agric，1980，31：625-632.

［7］AOAC. Official methods of analysis［M］. Arilington, Washington DC, USA, 1990.

［8］ZINN R A, OWENS F N. A rapid procedure for purine measurement and its use for estimating net ruminal protein synthesis［J］. Can J Anim Sci，1986，66：157-166.

［9］FAICHNEY G J. The use of markers to partition digestion within the gastro-intestinal tract of ruminants［M］. MCDONALD I W and WARNER A C I, ed. University of New England Publishing Unit, Armidale, Australia, 1975.

［10］KOENIG K M, IVAN M, TEFEREDEGNE B T, et al. Effect of dietary enterolobium cyclocarpum on microbial protein flow and nutrient digestibility in sheep maintained fauna-free, with total mixed fauna or with entodinium caudatum monofauna［J］. Br J Nutr，2007，98：504-516.

［11］AHVENJRVI S, VANHATALO, HUHTANEN P. Supplementing barley or rapeseed meal to dairy cows fed grass-red clover silage: I. Rumen degradability and microbial flow［J］. J Anim Sci，2002，80：2176-2187.

［12］BRODERICK G A, MERCHEN N R. Markers for quantifying microbial protein synthesis in the rumen［J］. J Dairy Sci，1992,75：2618-2632.

［13］PEOPLES M B, BERGERSEN F J, TURNER G L, et al. Use of the natural enrichment of ¹⁵N in plant available soil N for the measurement of symbiotic N2 fixation. In: EA IA (ed) Stable isotopes in plant nutrition, soil fertility and environmental studies［C］. IAEA，Vienna，1991: 117129.

［14］WATTIAUX M A, REED J D. Fractionation of nitrogen isotopes by mixed ruminal bacteria［J］. J Anim Sci，1995，73：257-266.

［15］BURRIS R H, WILSON P W. Methods for measurement of nitrogen fixation［M］. In methods in enzymology. Vol. 4. COLOWICH S P and KAPLAN N O, ed. Academic Press, New York, NY. 1957：355.

［16］CECAVA M J, MERCHEN N R, GAY L C, et al. Composition of ruminal bacteria harvested from steers as influenced by dietary energy level, feeding frequency, and isolation techniques［J］. J Dairy Sci，1990，73：2480-2488.

［17］BRODERICK G A, MERCHEN N R. Markers for quantifying microbial protein synthesis in the rumen［J］. J Dairy Sci，1992，75：2618-2632.

［18］CARRO M D, MILLER E L. Comparison of microbial markers (¹⁵N and purine bases) and bacterial isolates for the estimation of rumen microbial protein synthesis［J］. Anim Sci，2002，75：315-321.

［19］CRAIG W M, BRODERICK G A, RICKER D B. Quantitation of microorganisms associated with the particulate phase of ruminal ingesta［J］. J Nutr，1987，117：56.

［20］LEGAY-CARMIER F, BAUCHART D. Distribution of bacteria in the rumen contents of dairy cows given a diet supplemented with soyabean oil［J］. Br J Nutr，1989，61：725.

［21］GONZALEZ-RONQUIIIO M, BALCELLS J, BELENGUER A, et al. A comparison of purine derivatives excretion with conventional methods as indices of microbial yield in dairy cows［J］. J Dairy Sci，2004，87：2211-2221.

［22］CECAVA M J, MERCHEN N R, BERGER L L, et al. Effects of dietary energy level and protein source on nutrient digestion and ruminal nitrogen metabolism in steers［J］. J Anim Sci，1991，69：2230-2243.

［23］CALSAMIGLIA S, STERN M D, FRIKINS J L. Comparison of nitrogen-15 and purines as microbial markers in continous culture［J］. J Anim Sci，1996，74：1375-1381.

［24］FIRKINS J L, LEWIS S M, MONTGOMERY L, et al. Effects of feed intake and dietary urea concentration on ruminal dilution rate and efficiency of bacterial growth in steers［J］. J Dairy Sci，1987，70：2312-2321.

［25］PEREZ J F, BALCELLS J, GUADA J A, et al. Rumen microbial production estimated either from urinary purine derivative excretion or from direct measurements of ¹⁵N and purine bases as microbial markers: effect of protein source and rumen bacteria isolates［J］. Anim Sci，1997，65：225-236.

［26］SMITH R H, MCALLAN A B, HEWITT P, et al. Estimation of amounts of microbial and dietary nitrogen compounds entering the duodenum of cattle［J］. J Agric Sci，1978，90：557-568.

［27］HRISTOV A N, MCALLISTER T A, OUELLET D R, et al. Comparison of purines and nitrogen-15 as microbial flow markers in beef heifers fed barley- or corn-based diets［J］. Can J Anim Sci，2005，85：211-222.

［28］ARC. The nutrient requirements of ruminant livestock［M］. Suppl. No.1, Commonwealth Agricultural Bureaux, Slough, 1984.

［29］REYNAL S M, BRODERICK G A, BEARZL C. Comparison of four markers for quantifying microbial protein flow from the rumen of lactating dairy cows［J］. J Dairy Sci，2005，88：4065-4082.

［30］USHIDA K, LASSALAS B, JOUANY J P. Determination of assay parameters for RNA analysis in bacterial and duodenal samples by spectrophotometry influence of sample treatment and preservation［J］. Reprod Nutr Dev，1985，25：1037-1046.

［31］AHARONI Y, TAGARI H. Use of nitrogen- 15 determinations of purine nitrogen fraction of digesta to define nitrogen metabolism traits in the rumen［J］. J Dairy Sci，1991，74：2540-2547.

[1]	萨茹丽, 杨斌, 格根哈斯, 钱琳娜, 赵世华. 手掌参多糖提取物对氧化应激舍饲肉羊生产性能、抗氧化机能及肉品质的影响[J]. 畜牧兽医学报, 2020, 51(9): 2187-2196.
[2]	富丽霞, 马涛, 刁其玉, 成述儒, 宋雅喆. 精料类型对肉羊饲粮养分消化率及尿嘌呤衍生物排出量的影响[J]. 畜牧兽医学报, 2018, 49(7): 1394-1404.

应用¹⁵N和嘌呤估测肉羊微生物N产量的研究

Study on Microbial N Yield Measured with ¹⁵N and Purine Bases in Mutton Sheep

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

本文评价