急弯棘豆内生真菌的分离与鉴定

畜牧兽医学报 ›› 2010, Vol. 41 ›› Issue (7): 902-908.doi:

急弯棘豆内生真菌的分离与鉴定

路浩¹，马尧¹，赵宝玉¹*，来航线²，李蓉¹，卢围¹，荣杰¹

1.西北农林科技大学动物医学院，杨凌 712100；2. 西北农林科技大学资源环境学院，杨凌 712100

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-07-20 发布日期:2010-07-20
通讯作者: 赵宝玉

Isolation and Identification of Endophytic Fungus from Oxytropis deflexa

LU Hao¹, MA Yao¹, ZHAO Bao-yu¹*, LAI Hang-xian², LI Rong¹, LU Wei¹, RONG Jie¹

1.College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China;2. College of Resources and Environment, Northwest A&F University, Yangling 712100, China

Received:1900-01-01 Revised:1900-01-01 Online:2010-07-20 Published:2010-07-20

摘要/Abstract

摘要： 为探明我国青海主要疯草类植物——急弯棘豆中是否含有内生真菌，本研究对采自青海的急弯棘豆样品采用组织分离法对内生真菌进行分离，运用形态学和分子生物学方法对筛选到的内生真菌菌株进行种属鉴定。结果表明，从采集的急弯棘豆样品中共分离获得3株内生真菌，分别命名为EFJ-3、EFJ-4和EFG-8，通过形态学和分子生物学方法确定这3株内生真菌分别为分层镰刀菌、褶皱裸孢壳菌和焦曲霉。结果提示，我国青海地区急弯棘豆植株中存在多种内生真菌。

Abstract: To explore whether endophytic fungus exist in Oxytropis deflexa, the main locoweed species in Qinghai Province of China, endophytic fungus was isolated and cultured from O. deflexa by tissue separation, species and genus of endophytic fungus was identified by morphology and molecular biology. Three strains endophytic fungus were isolated and obtained from O. deflexa, and named EFJ-3, EFJ-4 and EFG-8, respectively. Morphology evidence and sequence analysis of the ITS region suggested that EFJ-3 was Fusarium proliferatum, EFJ-4 was Emericella rugulosa, EFG-8 was Aspergillus ustus. The result showed that endophytic fungal existed in O. deflexa locoweeds in Qinghai Province.

路浩;马尧;赵宝玉;来航线;李蓉;卢围;荣杰. 急弯棘豆内生真菌的分离与鉴定[J]. 畜牧兽医学报, 2010, 41(7): 902-908.

LU Hao;MA Yao;ZHAO Bao-yu;LAI Hang-xian;LI Rong;LU Wei;RONG Jie. Isolation and Identification of Endophytic Fungus from Oxytropis deflexa[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(7): 902-908.

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