Abstract:

In order to evaluate an avian influenza virusCK/SX/2/06(H5N1), which was isolated from a poultry farm in Shanxi province. HAHI test, RTPCR, IVPI, antigenic analysis, sequencing and phylogenic analysis, aquatic bird and BALB/c mice infection test were fulfilled in the study. The virus were H5N1 subtype highly pathogenic avian influenza virus (HPAIV), which showed big diversity to reference strain GS/GD/1/96(H5N1) in China in phylogenic and antigenic analysis. So it was defined as antigenicdrifted strain, the same viruses established a new clade, Shanxi chickenlike strain, compared with the same subtype viruses which caused H5N1 HPAI outbreak in China in recent years. The virus didn′t infected SPF duck and showed low pathogenic to mammal. These results indicated that new clade and antigenicdrifted viruses Shanxilike strain appeared in China, so we should adjust the measurement and strategy to prevent and control the disease.