朊蛋白在宁夏滩羊不同组织中表达的免疫组化研究

畜牧兽医学报 ›› 2010, Vol. 41 ›› Issue (3): 301-304.doi:

朊蛋白在宁夏滩羊不同组织中表达的免疫组化研究

许立华^1，2 ，康晓东³，涂健¹ ，张珠明^1，2，杨建民¹，周向梅¹，尹晓敏¹，杨利峰¹，赵德明¹*

中国农业大学动物医学院国家动物海绵状脑病实验室，北京 100193；2.宁夏大学农学院，银川 750021；3. 宁夏农林科学院畜产工程技术中心，银川 750004

收稿日期:2009-09-15 修回日期:1900-01-01 出版日期:2010-03-24 发布日期:2010-03-24

Imunohistochemical Study on PrP Expression in Different issues of Tan Sheep in Ningxia

XU Lihua^{1 ，2} ，KANG Xiaodong³，TU Jian¹ ，ZHANG Zhuming^1，2，YANG Jianmin¹，ZHOU Xiangmei¹，YIN Xiaomin¹，YANG Lifeng¹，ZHAO Deming ¹*

1.National Animal Transmissible Spongiform Encephalopathies Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China；2.College of Agriculture,Ningxia University, Yinchuan 750021, China；3.Technology Center of Animal Products,Ningxia Academy of Agriculture and Forestry Sciences， Yinchuan 750004, China

Received:2009-09-15 Revised:1900-01-01 Online:2010-03-24 Published:2010-03-24
Contact: ZHAO Deming

摘要/Abstract

摘要： 作者运用免疫组织化学技术，首次对我国一类保护物种宁夏滩羊各组织中朊蛋白（PrP）的分布进行定性、定位研究。结果显示，PrP在滩羊的大脑、脑干、小脑、心脏、肝脏、脾脏、肾脏及淋巴结均有表达，尤其在脾脏、肾脏、脑干、淋巴结中的表达量丰富，而肺脏检测结果为阴性。此研究确定了朊蛋白在滩羊各组织中的表达与分布状况，并优化了朊蛋白检测的技术条件，为羊痒病的诊断与检测提供技术支持。

Abstract: In the present experiment, a qualitative detection was implemented to determine distribution and location of prion protein（PrP） in different tissues of Ningxia Tan sheep, which is one of the protected species of classⅠ. The result indicated that PrP has a wide range of expression in different tissues, such as cerebrum, brain stem, cerebellum, heart, liver, spleen, kidney, lymph node and so on. Especially, prion protein has a higher expression level in brain stem, spleen, kidney and lymph node than other tissues. We also got a negative result in the samples from lung. This study confirmed the status of distribution of PrP in different tissues from Tan sheep. In addition, the study provided a technical support to diagnose and detect scrapie by optimizing immunohistochemical method.

许立华;;康晓东;涂健;张珠明;杨建民;周向梅;尹晓敏;杨利峰;赵德明. 朊蛋白在宁夏滩羊不同组织中表达的免疫组化研究[J]. 畜牧兽医学报, 2010, 41(3): 301-304.

XU Lihua ;;KANG Xiaodong;TU Jian ;ZHANG Zhuming;YANG Jianmin;ZHOU Xiangmei;YIN Xiaomin;YANG Lifeng;ZHAO Deming . Imunohistochemical Study on PrP Expression in Different issues of Tan Sheep in Ningxia[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(3): 301-304.

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