牛肺泡巨噬细胞微小RNA的克隆与鉴定

畜牧兽医学报 ›› 2009, Vol. 40 ›› Issue (9): 1410-1413.doi:

牛肺泡巨噬细胞微小RNA的克隆与鉴定

徐广贤，张艳，贾浩，周萍，王玉炯*

宁夏大学生命科学学院，银川 750021

收稿日期:2008-11-04 修回日期:1900-01-01 出版日期:2009-09-24 发布日期:2009-09-24
通讯作者: 王玉炯

Cloning and Identification of MicroRNA from Bovine Alveolar Macrophage

XU Guangxian, ZHANG Yan, JIA Hao, ZHOU Ping, WANG Yujiong*

College of Life Science, Ningxia University, Yinchuan 750021, China

Received:2008-11-04 Revised:1900-01-01 Online:2009-09-24 Published:2009-09-24
Contact: WANG Yujiong

摘要/Abstract

摘要：

MicroRNA（miRNA）是一类长19~26个核苷酸的内源性非编码单链小分子RNA，其异常表达将会导致生理的异常和疾病的产生。为了研究miRNAs在牛肺泡巨噬细胞抗菌机制中的作用，构建了牛肺泡巨噬细胞miRNA cDNA文库，从438个克隆中筛选出22个miRNAs，其中有19 个miRNAs在牛的miRBase数据库中没有发现，但是有8个miRNAs与人和（或）鼠中的序列完全同源，因此，将它们命名为BtamiR141、BtamiR187、BtamiR191、BtamiR448、BtamiR589、BtamiR873、BtamiR463 和BtamiR562；另外有11个miRNAs在所有miRBase数据库中都没有发现，有待进一步研究。这些数据为研究miRNAs调控牛肺泡巨噬细胞抗菌机制奠定了基础。

Abstract:

MicroRNAs (miRNAs) are a class of noncoding singlestranded RNA that play vital roles in many biological processes by regulating the expression of genes. To study the antibacterial mechanism of microRNAs in bovine alveolar macrophage, a cDNA library of microRNAs was constructed. By sequencing, 22 microRNAs have been screened in 438 clones. There are 19 microRNAs which have not been reported in miRBase, and 8 of them were sequence homology with human and rat. They are respectively named BtamiR141, BtamiR187, BtamiR191, BtamiR448, BtamiR589, BtamiR873, BtamiR463 and BtamiR562 by the rules. 11 others have not been found in any miRBase and should be further studied.

徐广贤;张艳;贾浩;周萍;王玉炯. 牛肺泡巨噬细胞微小RNA的克隆与鉴定[J]. 畜牧兽医学报, 2009, 40(9): 1410-1413.

XU Guangxian;ZHANG Yan;JIA Hao;ZHOU Ping;WANG Yujiong. Cloning and Identification of MicroRNA from Bovine Alveolar Macrophage[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(9): 1410-1413.

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