基于线粒体控制区的鸡不同杂交组合遗传多样性研究

doi:10.11843/j.issn.0366-6964.2021.06.008

畜牧兽医学报 ›› 2021, Vol. 52 ›› Issue (6): 1523-1534.doi: 10.11843/j.issn.0366-6964.2021.06.008

基于线粒体控制区的鸡不同杂交组合遗传多样性研究

唐修君^1,2,3, 樊艳凤^1,3, 贾晓旭^1,3, 葛庆联^1,3, 陆俊贤^1,3, 马丽娜^1,3, 韩威^1,3, 高玉时^1,3*

1. 中国农业科学院家禽研究所, 扬州 225125;
2. 南京农业大学动物科技学院, 南京 210095;
3. 江苏省家禽遗传育种重点实验室, 扬州 225125

收稿日期:2020-11-23 出版日期:2021-06-23 发布日期:2021-06-22
通讯作者: 高玉时,主要从事家禽遗传育种与质量安全研究,E-mail:gaoys100@sina.com
作者简介:唐修君(1981-),女,江苏射阳人,副研究员,博士生,主要从事家禽遗传育种和品质评价研究,E-mail:tangxj0918@126.com;樊艳凤(1988-),女,河北沧州人,硕士,主要从事家禽遗传育种和品质评价研究,E-mail:Fanyanfeng226@126.com。唐修君和樊艳凤为同等贡献作者
基金资助:
国家自然科学基金（31702079；31672382；31501917；31372277）；江苏省六大人才高峰资助项目（NY-011）；江苏省现代农业—重点及面上项目（BE2018363）；江苏省家禽遗传育种重点实验室资助项目（JQLAB-ZZ-202002）

Study on Genetic Diversity in Different Hybrid Combinations of Chickens Based on Mitochondrial Control Region

TANG Xiujun^1,2,3, FAN Yanfeng^1,3, JIA Xiaoxu^1,3, GE Qinglian^1,3, LU Junxian^1,3, MA Lina^1,3, HAN Wei^1,3, GAO Yushi^1,3*

1. Institute of Poultry, Chinese Academy of Agricultural Sciences, Yangzhou 225125, China;
2. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
3. Key Laboratory for Poultry Genetics and Breeding of Jiangsu Province, Yangzhou 225125, China

Received:2020-11-23 Online:2021-06-23 Published:2021-06-22

摘要/Abstract

摘要： 旨在探讨鸡不同杂交组合线粒体控制区（mtDNA D-loop区）的遗传多样性和单倍型特性。选取固始鸡和隐性白羽鸡及其正、反交F1代、藏鸡以及F2代等6个群体共387个个体的mtDNA D-loop区进行测序，分析其遗传规律和单倍型特性，并与不同红色原鸡亚种进行聚类，分析其母系起源。结果显示，6个群体D-loop区全序列大小为1 231 bp，共检测到28个多态位点和1个C碱基缺失，共构成19种单倍型，分为A、B、C和E 4个单倍型群，其中，固始鸡和反交F1代主要为A、C单倍型，固始鸡A、C单倍型比例分别为53.42%和46.58%，反交F1代A、C单倍型比例分别为50.75%和49.25%；隐性白羽鸡、正交F1代和F2代优势单倍型均为E单倍型，占比分别为48.89%、48.84%和50.00%。6个鸡群体单倍型多样度（Hd）在0.496~0.729之间，核苷酸多样度（Pi）在0.003 40~0.005 41之间，Hd值和Pi值最大的均为正交F1代，其次为隐性白羽鸡和F2代，固始鸡和反交F1代群体遗传多样性接近。聚类分析显示，A、B单倍型群与滇南亚种交叉聚为一枝；E单倍型群与印度亚种交叉聚为一枝；C单倍型群与印度亚种、指名亚种、印尼亚种以及滇南亚种聚为一枝。结果提示，mtDNA D-loop区遵循严格的母系遗传，后代的遗传多样性和单倍型比例与其母本基本一致；我国家鸡群体具有多个红色原鸡母系起源，且主要起源于原鸡滇南亚种。

关键词: 鸡, 线粒体控制区, 遗传多样性, 遗传起源

Abstract: The aim of this study was to investigate the genetic diversity and haplotype characteristics of the mitochondrial control region (mtDNA D-loop region) in different hybrid combinations of chickens. A total of 387 individual's mtDNA D-loop regions from 6 populations of Gushi chickens, Recessive White feather chickens and their reciprocal cross F1 generations, Tibetan chickens and F2 generations were sequenced to analyze their genetic laws and haplotype characteristics. Their maternal origins were analyzed by clustering with different red jungle fowl subspecies. The results showed that the full sequence of the D-loop region of the 6 populations was 1 231 bp, and a total of 28 polymorphic sites and one C-base deletion mutation were detected, constituting 19 haplotypes which were divided into 4 haplotype groups, A, B, C and E. Among them, the Gushi chicken and the reverse cross F1 generation were mainly A and C haplotypes, the proportions of A and C haplotypes in Gushi chicken were 53.42% and 46.58%, respectively, and the proportions of A and C haplotypes in reverse cross F1 generation were 50.75% and 49.25%, respectively. The dominant haplotype of Recessive White feather chicken, orthogonal F1 generation and F2 generation was E, accounting for 48.89%, 48.84% and 50.00%, respectively. The haplotype diversity (Hd) of the 6 chicken populations ranged from 0.496 to 0.729, and the nucleotide diversity (Pi) ranged from 0.003 40 to 0.005 41. The largest Hd and Pi values were detected in the orthogonal F1 generation, followed by the Recessive White feather chicken and the F2 generation, and the genetic diversities of the Gushi chicken and the reverse cross F1 generation populations were close to each other. Cluster analysis showed that the haplotype group A, B and Gallus gallus spadiceus were clustered in one group; haplotype group E and Gallus gallus murghi were clustered in another group; haplotype group C was clustered with 4 subspecies of red jungle fowl, including Gallus gallus murghi, Gallus gallus spadiceus, Gallus gallus gallus and Gallus gallus bankiva. The present study demonstrated that the mtDNA D-loop region followed strict matrilineal inheritance, and the genetic diversity and haplotype ratio of the progeny were basically the same as those of their female parent; the local chicken breeds in China had multiple maternal lineages, and mainly originated from Gallus gallus spadiceus.

Key words: chicken, mitochondrial control region, genetic diversity, genetic origins

中图分类号:

S831.2

唐修君, 樊艳凤, 贾晓旭, 葛庆联, 陆俊贤, 马丽娜, 韩威, 高玉时. 基于线粒体控制区的鸡不同杂交组合遗传多样性研究[J]. 畜牧兽医学报, 2021, 52(6): 1523-1534.

TANG Xiujun, FAN Yanfeng, JIA Xiaoxu, GE Qinglian, LU Junxian, MA Lina, HAN Wei, GAO Yushi. Study on Genetic Diversity in Different Hybrid Combinations of Chickens Based on Mitochondrial Control Region[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(6): 1523-1534.

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基于线粒体控制区的鸡不同杂交组合遗传多样性研究

Study on Genetic Diversity in Different Hybrid Combinations of Chickens Based on Mitochondrial Control Region

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