致仔猪脑膜炎猪链球菌的分离鉴定

doi:10.11843/j.issn.0366-6964.2019.01.017

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (1): 143-150.doi: 10.11843/j.issn.0366-6964.2019.01.017

致仔猪脑膜炎猪链球菌的分离鉴定

陈申申^1,2, 马家乐^1,2, 张新锋^1,2, 曲向阳³, 潘子豪^1,2, 姚火春^1,2*

1. 南京农业大学动物医学院, 南京 210095;
2. 南京农业大学OIE猪链球菌病参考实验室, 南京 210095;
3. 环山集团养猪事业部, 青岛 266071

收稿日期:2018-06-04 出版日期:2019-01-23 发布日期:2019-01-23
通讯作者: 姚火春,主要从事动物及人兽共患疫病病原学、细菌及病毒致病机制、病原与宿主互作关系、动物疾病防控研究,E-mail:yaohch@njau.edu.cn
作者简介:陈申申(1993-),女,江苏泰州人,硕士,主要从事微生物与免疫方面的研究,E-mail:15295214923@163.com
基金资助:
上海市科技兴农重点攻关项目[沪农科攻字（2016）第6-2-1号]

Isolation and Identification of Streptococcus suis Causing Meningitis in Piglets

CHEN Shenshen^1,2, MA Jiale^1,2, ZHANG Xinfeng^1,2, QU Xiangyang³, PAN Zihao^1,2, YAO Huochun^1,2*

1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;
2. OIE Reference Laboratory for Swine Streptococcus of Nanjing Agricultural University, Nanjing 210095, China;
3. The Hog-rasing Department of Huanshan Group, Qingdao 266071, China

Received:2018-06-04 Online:2019-01-23 Published:2019-01-23

摘要/Abstract

摘要：

旨在从脑膜炎症状仔猪的脑组织及其他脏器中分离鉴定病原菌。采集患病仔猪脏器分离病原，通过PCR及血清凝集试验鉴定其种属及血清型；透射电镜观察分离株的超微结构；经多序列位点分型分析，鉴定其ST型；通过测定最小抑菌浓度分析其耐药性；经小鼠攻毒试验分析其毒力。结果：最终从患脑膜炎仔猪的脑组织中分离到一株猪链球菌，血清型为9型，将其命名为WH1609。电镜结果显示WH1609有荚膜结构。多位点序列分型结果表明WH1609属于一个新的ST型。耐药性试验结果表明WH1609对多种抗生素耐药；对万古霉素、青霉素及氨苄西林敏感。BALB/c小鼠毒力试验测得LD₅₀为1.89×10²CFU·mL^-1，病理组织学观察结果显示WH1609感染小鼠可出现明显的脑组织病变。本研究从患脑膜炎的仔猪脑组织中分离到一株猪链球菌9型强毒株，其在BALB/c小鼠的半数致死量为已发现的猪链球菌强毒株的10⁵倍以上，对猪链球菌致脑膜炎分子机制的研究有重要的意义。

Abstract:

The purpose of this study was to isolate and identify pathogenic bacteria from brain tissues and other organs of piglets suffering from meningitis. The species and serotype of the isolated strain were identified by PCR and serum agglutination test. The ultrastructure of the isolated strain was observed by transmission electron microscope, and the sequence type (ST) was determined by multilocus sequence typing tests (MLST). Minimal inhibitory concentration (MIC) was determined to analyze the antibiotic resistance of the isolated strain, while the virulence was detected by mice infection experiments. Results were as follows:a strain of Streptococcus suis was isolated from the brain tissue of piglets with meningitis which belongs to serotype 9 and named WH1609. The result of electron microscope showed that WH1609 had capsular structure. The result of MLST showed that WH1609 belonged to a new ST. WH1609 was a multi-drug resistant strain but sensitive to vancomycin, penicillin and ampicillin. The LD₅₀ of BALB/c mice infected with WH1609 was 1.89×10² CFU·mL^-1, and histopathological observation showed that the infected mice had obvious lesion in brain tissue. All the results suggest that a virulent strain of Streptococcus suis 9 isolated from the brain tissue of piglets suffering from meningitis exhibited a 10⁵ times higher LD₅₀ than that of other Streptococcus suis virulent strains, which is of great value to the study of the molecular mechanism meningitis caused by Streptococcus suis.

中图分类号:

S852.611

陈申申, 马家乐, 张新锋, 曲向阳, 潘子豪, 姚火春. 致仔猪脑膜炎猪链球菌的分离鉴定[J]. 畜牧兽医学报, 2019, 50(1): 143-150.

CHEN Shenshen, MA Jiale, ZHANG Xinfeng, QU Xiangyang, PAN Zihao, YAO Huochun. Isolation and Identification of Streptococcus suis Causing Meningitis in Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 143-150.

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致仔猪脑膜炎猪链球菌的分离鉴定

Isolation and Identification of Streptococcus suis Causing Meningitis in Piglets

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