玉米赤霉烯酮对小鼠T淋巴细胞体外活化、增殖的影响

doi:10.11843/j.issn.0366-6964.2017.07.021

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (7): 1357-1364.doi: 10.11843/j.issn.0366-6964.2017.07.021

玉米赤霉烯酮对小鼠T淋巴细胞体外活化、增殖的影响

蔡国栋^1,2, 孙凯¹, 项自来¹, 王玲¹, 邹辉¹, 顾建红¹, 袁燕¹, 刘学忠¹, 刘宗平^1,2, 卞建春^1,2*

1. 扬州大学兽医学院, 扬州 225009;
2. 江苏省动物重要疫病与人兽共患病防控协同创新中心, 扬州 225009

收稿日期:2016-12-29 出版日期:2017-07-23 发布日期:2017-07-23
通讯作者: 卞建春(1965-),男,博士,教授,主要从事动物营养代谢病与中毒病研究,Tel:0514-87979042,E-mail:jcbian@yzu.edu.cn
作者简介:蔡国栋(1992-),安徽蚌埠人,硕士生,主要从事动物营养代谢病与中毒病研究,E-mail:2663740180@qq.com
基金资助:
国家重点研发计划支持项目（2016YFD0501208）；江苏省普通高校自然科学基金（08KJD230002）；江苏高校优势学科建设工程资助项目（PAPD）

The Effects of Zearalenone on the Activation and Proliferation of Mouse T-lymphocytesin vitro

CAI Guo-dong^1,2, SUN Kai¹, XIANG Zi-lai¹, WANG Ling¹, ZOU Hui¹, GU Jian-hong¹, YUAN Yan¹, LIU Xue-zhong¹, LIU Zong-ping^1,2, BIAN Jian-chun^1,2*

1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;
2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China

Received:2016-12-29 Online:2017-07-23 Published:2017-07-23

摘要/Abstract

摘要：

为揭示镰刀菌毒素之一的玉米赤霉烯酮（ZEA）的免疫抑制作用机制，作者拟研究ZEA对刀豆蛋白A（Con A）介导的小鼠离体T淋巴细胞体外活化、增殖的影响。以Con A作为T淋巴细胞活化刺激剂，以不同浓度ZEA（0、10、20、40 μmol·L^-1）染毒处理细胞后，利用流式细胞术检测T淋巴细胞早期活化标志CD69分子及中期活化标志CD25分子表达情况，用CCK-8法检测T淋巴细胞的增殖情况。结果显示：细胞分别培养48、72、96 h后，Con A组与细胞空白组相比均产生了明显的刺激增殖效应（P<0.01）。与Con A对照组相比，ZEA染毒可明显抑制Con A刺激引起的T淋巴细胞的增殖作用。除10 μmol·L^-1 ZEA组在72和96 h时间段差异显著外（P<0.05），其余染毒组在各时间段均差异极显著（P<0.01），并呈浓度-效应关系。细胞加入Con A分别活化6、30 h后，Con A组与细胞空白组相比，早期活化分子CD69和中期活化分子CD25明显升高，提示细胞发生明显的活化效应。与Con A对照组相比，当ZEA染毒浓度为10 μmol·L^-1时，T细胞早期活化标志分子CD69和中期活化分子CD25的表达明显抑制，差异均极显著（P<0.01），当ZEA浓度为20、40 μmol·L^-1时，CD69和CD25的表达进一步抑制（P<0.01），并呈剂量依赖性。研究结果表明，ZEA对动物免疫抑制作用的产生与其可直接抑制对小鼠T淋巴细胞的活化和增殖有关。

Abstract:

In order to reveal the mechanism of the immunosuppressive effect of Fusarium toxin zearalenone (ZEA), this experiment had studied the effects of ZEA on the activation and proliferation of mouse T lymphocytes stimulated by concanavalin A (Con A) in vitro. After being treated with different concentrations of ZEA (0, 10, 20, 40 μmol·L^-1), CCK-8 method was used to detect the proliferation of T lymphocytes. At the same time, the expression levels of the early activation antigen CD69 and metaphase activation antigen CD25 were evaluated by flow cytometry. Results were as follows:Cells were cultured for 48, 72, 96 h, compared with group of cell blank, the group of Con A had obvious proliferating phenomenon (P <0.01). Compared with the group of Con A only, group of ZEA 10 μmoL·L^-1 had an obvious phenomenon of Inhibition of proliferation (P <0.05). When ZEA concentration was 20, 40 μmol·L^-1, the proliferation of T lymphocytes was further inhibited. The proliferation of T lymphocytes stimulated by Con A decreased obviously with the increased concentration of the ZEA which showed in a dose-dependent manner (P <0.01). Cells were stimulated by Con A for 6 or 30 hours. Compared with group of cell blank, the group of Con A had obvious activated phenomenon (P <0.01). Compared with the group of Con A only, group of ZEA 10 μmol·L^-1 had an obvious phenomenon of Inhibition of activation (P <0.01). When ZEA concentration was 20, 40 μmol·L^-1, the expression of CD69 and CD25 was further inhibited. The expression levels of the early activation antigen CD69 and metaphase activation antigen CD25 were significantly inhibited after being treated with the ZEA which showed in a dose-dependent manner (P <0.01). The immunosuppressive effect of ZEA to animals is related to its directly inhibition to the activation and proliferation of T lymphocytes in mouse.

中图分类号:

S859.87

蔡国栋, 孙凯, 项自来, 王玲, 邹辉, 顾建红, 袁燕, 刘学忠, 刘宗平, 卞建春. 玉米赤霉烯酮对小鼠T淋巴细胞体外活化、增殖的影响[J]. 畜牧兽医学报, 2017, 48(7): 1357-1364.

CAI Guo-dong, SUN Kai, XIANG Zi-lai, WANG Ling, ZOU Hui, GU Jian-hong, YUAN Yan, LIU Xue-zhong, LIU Zong-ping, BIAN Jian-chun. The Effects of Zearalenone on the Activation and Proliferation of Mouse T-lymphocytesin vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(7): 1357-1364.

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参考文献

[1] JI C, FAN Y, ZHAO L H. Review on biological degradation of mycotoxins[J]. Anim Nutr, 2016, 2(3):127-133.
[2] WANG Y, LIU S L, ZHENG H, et al. T-2 toxin, zearalenone and fumonisin B₁ in feedstuffs from China[J]. Food Addit Contam B, 2013, 6(2):116-122.
[3] YIN S T, MENG Q W, ZHANG B R, et al. Alleviation of zearalenone toxicity by modified halloysite nanotubes in the immune response of swine[J]. Food Addit Contam A, 2015, 32(1):87-99.
[4] CHENG Y H, WENG C F, CHEN B J, et al. Toxicity of different Fusarium mycotox ins on growth performance, immune responses and efficacy of a mycotoxin degrading enzyme in pigs[J]. Anim Res, 2006, 55(6):579-590.
[5] 刘海军, 刘可越. 猪无名高热综合症流行的原因及预防措施[J]. 安徽农业科学, 2007, 35(24):7476-7477.
LIU H J, LIU K Y. Research on the syndrome of pig with high temperature and its preventing measure[J]. Journal of Anhui Agricultural Sciences, 2007, 35(24):7476-7477. (in Chinese)
[6] LEE K H, HOLDORF A D, DUSTIN M L, et al. T cell receptor signaling precedes immunological synapse formation[J]. Science, 2002, 295(5559):1539-1542.
[7] REN Z H, DENG Y T, WANG Y C, et al. Effects of the Fusarium toxin zearalenone and/or deoxynivalenol on the serum IL-1, IL-4, and C3 levels in mice[J]. Food Agric Immunol, 2016, 27(3):414-421.
[8] REN Z H, ZHOU R, DENG J L, et al. Effects of the Fusarium toxin zearalenone (ZEA) and/or deoxynivalenol (DON) on the serum IgA, IgG and IgM levels in mice[J]. Food Agric Immunol, 2014, 25(4):600-606.
[9] YANG L J, YANG W R, FENG Q, et al. Effects of purified zearalenone on selected immunological measurements of blood in post-weaning gilts[J]. Anim Nutr, 2016, 2(3):142-148.
[10] 范小龙, 刘长永, 剡海阔, 等. 玉米赤霉烯酮对小鼠脾淋巴细胞增殖及分泌细胞因子的影响[J]. 中国畜牧兽医, 2011, 38(5):48-54.
FAN X L, LIU C Y, YAN H K, et al. Effects of ZEN on in vitro proliferation and cytokines production of spleen lymphocyte in mice[J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(5):48-54. (in Chinese)
[11] 潘顺叶, 王光光, 范文桐, 等. 玉米赤霉烯酮诱导CTLL-2细胞凋亡途径的研究[J]. 中国兽医科学, 2016, 46(3):398-402.
PAN S Y, WANG G G, FAN W T, et al. Apoptosis induced by zearalenone in CTLL-2 cells[J]. Chinese Veterinary Science, 2016, 46(3):398-402. (in Chinese)
[12] POLASKY C, WEIGEND S, SCHRADER L, et al. Non-specific activation of CD8α-characterised γδ T cells in PBL cultures of different chicken lines[J]. Vet Immunol Immunopathol, 2016, 179:1-7.
[13] HAMMERICH L, WARZECHA K T, STEFKOVA M, et al. Cyclic adenosine monophosphate-responsive element modulator alpha overexpression impairs function of hepatic myeloid-derived suppressor cells and aggravates immune-mediated hepatitis in mice[J]. Hepatology, 2015, 61(3):990-1002.
[14] XIONG Y, ZHANG S, LU J, et al. Investigation of effects of farrerol on suppression of murine T lymphocyte activation in vitro and in vivo[J]. Int Immunopharmacol, 2013, 16(2):313-321.
[15] XIE J, ZHANG J, WU H M, et al. The influences of age on T lymphocyte subsets in C57BL/6 mice[J]. Saudi J Biol Sci, 2017, 24(1):108-113.
[16] SONG B C, HUANG G R, TONG C Y, et al. Gossypol suppresses mouse T lymphocytes via inhibition of NFκB, NFAT and AP-1 pathways[J]. Immunopharmacol Immunotoxicol, 2013, 35(5):615-621.
[17] MARIN D E, TARANU I, BURLACU R, et al. Effects of zearalenone and its derivatives on porcine immune response[J]. Toxicol Vitro, 2011, 25(8):1981-1988.
[18] MARIN D E, TARANU I, BURLACU R, et al. Effects of zearalenone and its derivatives on the innate immune response of swine[J]. Toxicon, 2010, 56(6):956-963.
[19] 徐占云, 秦睿玲, 褚耀诚, 等. 枸杞多糖对雏鸡淋巴细胞体外增殖及分泌IL-2的影响[J]. 畜牧兽医学报, 2013, 44(2):322-328.
XU Z Y, QIN R L, CHU Y C, et al. Effects of Lycium barbarum polysaccharide on cell proliferation of chicken lymphocytes and IL-2 excretion in vitro[J]. Acta Veterinaria et Zootechnica Sinica, 2013, 44(2):322-328. (in Chinese)
[20] CRASTON R, KOH M, DERMOTT A M, et al. Temporal dynamics of CD69 expression on lymphoid cells[J]. J Immunol Methods, 1997, 209(1):37-45.
[21] FULCHER D A, WONG S W J. Carboxyfluorescein succinimidyl ester-based proliferative assays for assessment of T cell function in the diagnostic laboratory[J]. Immunol Cell Biol, 1999, 77(6):559-564.
[22] KRECHETOVA L V, VTORUSHINA V V, NIKOLAEVA M A, et al. Expression of early activation marker CD69 on peripheral blood lymphocytes from pregnant women after first trimester alloimmunization[J]. Bull Exp Biol Med, 2016, 161(4):529-532.
[23] SANCHO D, GÓMEZ M, SÁNCHEZ-MADRID F. CD69 is an immunoregulatory molecule induced following activation[J]. Trends Immunol, 2005, 26(3):136-140.
[24] CORTÉS J R, SÁNCHEZ-DÍAZ R, BOVOLENTA E R, et al. Maintenance of immune tolerance by Foxp3⁺ regulatory T cells requires CD69 expression[J]. J Autoimmun, 2014, 55:51-62.
[25] LUND H, BOYSEN P, DEAN G A, et al. Interleukin-15 activated bovine natural killer cells express CD69 and produce interferon-γ[J]. Vet Immunol Immunopathol, 2012, 150(1-2):79-89.
[26] VAN LEEUWEN J E, SAMELSON L E. T cell antigen-receptor signal transduction[J]. Curr Opin Immunol, 1999, 11(3):242-248.
[27] WANG K P, GU J, NI X H, et al. CD25 signaling regulates the function and stability of peripheral Foxp3+ regulatory T cell derived from the spleen and lymph nodes of mice[J]. Mol Immunol, 2016, 76:35-40.
[28] CHIEN M W, LIN M H, HUANG S H, et al. Glucosamine modulates T cell differentiation through down-regulating N -linked Glycosylation of CD25[J]. Biol Chem, 2015, 290(49):29329-29344.
[29] YU J Y, ZHENG Z H, SON Y O, et al. Mycotoxin zearalenone induces AIF-and ROS-mediated cell death through p53-and MAPK-dependent signaling pathways in RAW264.7 macrophages[J]. Toxicol Vitro, 2011, 25(8):1654-1663.
[30] HOU Y J, ZHU C C, XU Y X, et al. Zearalenone exposure affects mouse oocyte meiotic maturation and granulosa cell proliferation[J]. Environ Toxicol, 2015, 30(10):1226-1233.
[31] ZHAO F, LI R, XIAO S, et al. Postweaning exposure to dietary zearalenone, a mycotoxin, promotes premature onset of puberty and disrupts early pregnancy events in female mice[J]. Toxicol Sci, 2013, 132(2):431-442.

玉米赤霉烯酮对小鼠T淋巴细胞体外活化、增殖的影响

The Effects of Zearalenone on the Activation and Proliferation of Mouse T-lymphocytesin vitro

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