GDF9下调转基因小鼠模型对繁殖与生长的影响

doi:10.11843/j.issn.0366-6964.2015.07.011

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (7): 1157-1162.doi: 10.11843/j.issn.0366-6964.2015.07.011

GDF9下调转基因小鼠模型对繁殖与生长的影响

习欠云，焦莉，肖敏，张永亮^*

(华南农业大学动物科学学院，广州 510642)

收稿日期:2014-11-26 出版日期:2015-07-23 发布日期:2015-07-23
通讯作者: 张永亮，教授，博导，主要从事动物生物化学与营养研究，E-mail：zhangyl@scau.edu.cn
作者简介:习欠云（1973-），男，江西樟树人，博士，副教授，主要从事动物生物化学与营养研究，E-mail：xqy0228@163.com；Tel：020-85284937
基金资助:
国家科技重大专项(2013ZX08007003-004)；转基因重点专项（2014ZX08009-48B）；广东省自然科学基金(S2013010013215)

The Effect of Transgenic Mice Model Down-regulated GDF9 on Reproduction and Growth

XI Qian-yun，JIAO Li，XIAO Min，ZHANG Yong-liang^*

(College of Animal Science，South China Agricultural University，Guangzhou 510642，China)

Received:2014-11-26 Online:2015-07-23 Published:2015-07-23

摘要/Abstract

摘要：

本研究旨在对转GDF9 shRNA基因小鼠进行扩群繁殖，统计分析转基因小鼠的产仔数及增重，检测GDF9 shRNA、GDF9表达水平以及血清中FSH和GH的水平，以观察GDF9的下调对转基因小鼠繁殖性能及生长发育的影响。通过RT-PCR方法，分别检测了GDF9 shRNA在体内的表达，以及下丘脑、垂体和卵巢中GDF9 mRNA表达水平；同时用ELISA检测了血液中FSH和GH水平。结果表明，扩群后得到16只GDF9 shRNA阳性小鼠，转基因GDF9 shRNA在小鼠体内广泛表达，转基因小鼠的产仔数与野生型小鼠相比差异显著（P<0.05）；转GDF9 shRNA阳性公鼠的体重比阴性公鼠高，且在第7周差异显著（P<0.05），而母鼠间体重无显著差异；与阴性小鼠相比，转GDF9 shRNA阳性小鼠GDF9基因的表达量在下丘脑、垂体中分别下降35.4%和39.8%，差异显著（P<0.05），在母鼠卵巢中表达水平差异不显著（P>0.05）；阳性母鼠血液中FSH水平比阴性母鼠高55%，差异极显著（P<0.01）。结果表明，GDF9基因下调可以提高动物繁殖性能，为提高大型牲畜繁殖力提供了有利的素材和靶点参考。

Abstract:

This experiment aims to detect the effect of down-regulation of GDF9 on reproduction and growth in GDF9 shRNA transgenic mice through statistical analysis of the litter size and weight gain of transgenic mice and detection of biomolecular and blood biochemical indexes such as GDF9 shRNA，GDF9 and serum FSH and GH level after reproduction.The levels of GDF9 shRNA and GDF9 mRNA expression were detected in hypothalamus，pituitary and ovary via RT-PCR，and serum FSH and GH level were examined using ELISA.The results showed that 16 GDF9 shRNA transgenic mice obtained were positive，and GDF9 shRNA in transgenic mice was widely expressed in various tissues.Litter sizes between transgenic and wild mice were significantly different (P<0.05)；Body weight of transgenic male mice was higher than that of control mice with significant difference (P<0.05) in the seventh week，and body weight of female mice did not show significant difference.Compared with the negative mouse，GDF9 gene expression in transgenic mice in hypothalamus and pituitary were decreased by 35.4% and 39.8% (P<0.05)，respectively.In transgenic female mice，GDF9 expression in ovaries was not significantly decreased (P>0.05)，but its serum FSH level was 55% higher than the control (P<0.01).The present study showed down-regulation expression of GDF9 gene can improve the ability of animal reproduction.It may provide a favorable material and molecular target for producing transgenic large livestock with high reproduction.

中图分类号:

S814.8

习欠云，焦莉，肖敏，张永亮. GDF9下调转基因小鼠模型对繁殖与生长的影响[J]. 畜牧兽医学报, 2015, 46(7): 1157-1162.

XI Qian-yun，JIAO Li，XIAO Min，ZHANG Yong-liang. The Effect of Transgenic Mice Model Down-regulated GDF9 on Reproduction and Growth[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1157-1162.

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GDF9下调转基因小鼠模型对繁殖与生长的影响

The Effect of Transgenic Mice Model Down-regulated GDF9 on Reproduction and Growth

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