畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (3): 402-406.doi: 10.11843/j.issn.0366-6964.2015.03.008

• 遗传繁育 • 上一篇    下一篇

绵羊磷脂酶C zeta基因对绵羊卵母细胞孤雌激活的初步研究

木尔扎提•阿勒腾别克,刘强,黄增文,米来•夏日甫,赛务加甫*   

  1. (石河子大学动物科技学院,石河子 832003)
  • 收稿日期:2014-10-28 出版日期:2015-03-23 发布日期:2015-03-23
  • 通讯作者: 赛务加甫,博士,主要从事转基因动物研究,E-mail 291016059@qq.com
  • 作者简介:木尔扎提?阿勒腾别克(1989-),男,哈萨克族,新疆阿勒泰人,硕士生,主要从事兽医产科学研究,E-mail:1379888527@qq.com
  • 基金资助:

    国家自然科学基金项目(31160524)

Preliminary Study Parthenogenetic Activation of Phospholipase C zeta (PLCζ) Gene of Sheep Oocytes

MU ERZATI•A Letengbieke,LIU Qiang,HUANG Zeng-wen,MI LAI•Xia Rifu,SAI Wujiafu*   

  1. (College of Animal Science and Technology,Shihezi University,Shihezi 832003,China)
  • Received:2014-10-28 Online:2015-03-23 Published:2015-03-23

摘要:

旨在应用绵羊磷脂酶C zeta(PLCζ)基因对绵羊卵母细胞进行激活,探究是否可以作为一种新的卵母细胞激活物质。本研究构建了pEGFP-N1-PLCζ重组质粒,并将质粒注入绵羊MⅡ期卵母细胞,观察及统计PLCζ对卵母细胞的激活效果和胚胎发育的影响。结果表明,pEGFP-N1-PLCζ重组质粒可在卵母细胞中表达,并可自发激活卵母细胞孤雌发育。

Abstract:

Sheep phospholipase C zeta (PLCζ) was used to activate sheep oocytes,to explore whether PLCζ can be used as a new kind of oocyte activator.A pEGFP-N1-PLCζ recombinant plasmid were constructed and then injected into MⅡ sheep oocytes.The effect of PLCζ on activation of oocytes and embryo development were observed and counted.The results showed that pEGFP-N1-PLCζ recombinant plasmid could express PLCζ in oocytes,and could spontaneously activate parthenogenetic development of sheep oocytes.

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