Acta Veterinaria et Zootechnica Sinica

Study on LINEs in Pig and Bovine Genomes

LI Dong-feng;LI Xiang-long;ZHOU Rong-yan;LI Lan-hui

2010, 41(5): 511-517. doi:

Abstract ( 632 )

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This experiment was conducted to study the distribution, origin, transfer pattern and interaction of L1 and RTE in bovine and pig genomes. Using the software of RepeatMasker and Censor, the content and distribution of L1 and RTE in bovine and pig genomes were detected. The average content and length of L1 are 7.89% and 529 bp in bovine genome, and 10.64% and 608 bp in pig genome. The average content and length of RTE in bovine genome are 11.83% and 433 bp, and the pig genome does not contain RTE sequence. According to the nucleotide divergence, we divided the L1 in bovine and pig genomes into 6 families and 14 subfamilies. The content of L1 in bovine and pig genomes reduced with the increasing of the GC content of every chromosome, same as the content of RTE in bovine genome. The RTE in bovine genome originated from an unknown species by horizontal transfer and did not from the antique mammals vertically. All the content and category data of L1 and RTE indicated the appearance of adaptive evolution in L1, RTE and between L1 and RTE.

The Cloning and Expression Analysis of One Abnormal Alternative Splice in Chicken Lmbr1

HUANG Yan-qun;CHEN Wen;LI Ning;DENG Xue-mei;KANG Xiang-tao

2010, 41(5): 518-523. doi:

Abstract ( 1010 )

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Based on the report that the abnormal splice form deleted exon 4 of Lmbr1/C7orf2 associated with human Acheiropodia and the conservation of the alternative splices among species to some degree, the identification and expression analysis of chicken Lmbr1 similar transcript variant were conducted. Primers spanning chicken Lmbr1 exon 4 were designed to clone the alternative splice deleted exon 4（Lmbr1-β）of chicken Lmbr1-α. The primers spanning exon 4 could detect the expression of two different transcripts of chicken Lmbr1 at the same time. The tissue expression analysis in one day-old chicks and 5-6 day-old embryos of White Leghorn showed that two transcripts could be detected in all tissues, and comparing with chicken Lmbr1-α, Lmbr1-β was a minor transcript and its expression level was weak. Comparison between the Lmbr1 expression of E3-E11 in polydactyly Silkies and four tetradactyly White Leghorn showed that both Lmbr1-α and Lmbr1-β expressed in all detected chicken embryo tissues, Lmbr1-α was the main transcript product and Lmbr1-β was a minor transcript product in very weak expression. No significant tissue expression difference was identified between the two breeds. It showed that Lmbr1-α containing exon 4 was the main transcript of Lmbr1, which expressed in all detected tissues of the chicken and developing embryos, the development of polydactyly in Silky was not related with the expression of chicken Lmbr1-α and Lmbr1-β.

Association between the SNPs of LYZ Gene and Growth,Laying Performance of Jinghai Yellow Chicken

HOU Qi-rui;WANG Jin-yu;WANG Hui-hua;LI Yuan;SHI Hui-qiang

2010, 41(5): 524-530. doi:

Abstract ( 668 )

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To discuss the relationship of LYZ gene with growth and laying performance traits, the second generation divergent lines of J+ and J-female bred by Jinghai yellow chicken breeding center were used in the present study. Three SNPs (G111A, T1426C, C1492T) in exon1 and exon2 were discovered by sequencing. Multi-contrast analysis showed that the birds with genotype AA were significantly higher than those with genotype GG and GA for body weight at 12 and 16 weeks of age, and smaller than those for age of first egg (P<0.05). The birds with genotype TT were significantly lower than those with other genotypes for body weight at 4 and 8 weeks of age and body weight of first egg, while the birds with genotype TN were significantly higher (P<0.05). Analysis results indicated that the genetic effects of haplotypes on body weight of 4 to 16 weeks of age and body weight of first egg were significant. Population genetic analysis indicated that the frequency of the genotypes were significantly different in the two lines (P≤0.05). It was concluded preliminarily that chicken LYZ gene had important effects on growth process in the chicken or it closely linked with those genes affecting growth.

Study on SNP of LXRα Gene Exon2 and Its Association with Some Meat Traits in Qinchuan Cattle

HUANG Lei;ZAN Lin-sen;WANG Hong-bao;LIU Hong-yu

2010, 41(5): 531-535. doi:

Abstract ( 690 )

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The aim of this study was to analyze the association between hereditary variation of bovine LXRα exon2 and some meat traits. Four hundred and ninty-seven of 18-20 months old Qinchuan steers under identical feeding conditions were selected randomly and variation of LXRα gene was analyzed by PCR-SSCP technique. The association between SNP locus and some meat traits was analyzed using the general linear model (GLM) in SPSS program. One polymorphic site T1530C was found (NC_007313). Genotypic variation had a significant effect on backfat thickness, carcass length and marbling score (P<0.05).The carcass length of individuals with BB and AB genotypes was significantly different from that of individuals with AA genotype (P<0.05). The marbling score and backfat thickness of individuals with BB genotype were significantly different from that of individuals with AA genotype (P<0.05). The results indicated that BB genotype was favorable genotype at this SNP locus and was highly associated with seven meat traits, which suggested that LXRα gene could be studied as a candidate gene for molecular marker assisted selection(MAS).

Study of Adjustment Factors for Standardizing Milking Records in Xinjiang Brown Cattle

FU Xue-feng;WANG Ya-chun;GUO Jun-qing;GUO Zhi-qin;CHENG Li-ming;ZHOU Guang-rui;HUANG Xi-xia;XU Yong

2010, 41(5): 536-542. doi:

Abstract ( 1126 )

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The objective of this study was to draw up the adjustment factor on 17 962 month milk yield records of Xinjiang Brown cattle. The lactation curve was fitted by five mathematical models (including the Wood′s incomplete gamma function model, inverse quadratic polynomial model, polynomial regression models (sextic), Ali-Schaeffer model and Wilmink model) with the nonlinear (NLIN) procedure, SAS(8.1) system. The results showed that the R2 values ranged from 0.834 0 to 0.921 4 for five models, depending on the model criteria, Inverse polynomial model was the best fitting model of lactation curves in this study, Lactation graph of different parities were drawn up, adjustment factors of milk production were drafted for Xinjiang Brown cattle. These results indicated that latest peak production occurred in first lactation for all models, while third lactation cows had the earliest day of peak production. With the growth of parity, milk production was increased continuously, while fifth parity cows had the highest milk production, and then gradually declined to agalorrhea, and means of 305-day milk yield was 4 445.3 kg.

Effects of Cumulus Cells on in vitro Maturation and ParthenogeneticDevelopment of Liaoning Cashmere Goats Oocytes

TIAN Chang-yong;YANG Jing-chao;MA Ze-fang;SONG Lian-xi

2010, 41(5): 543-548. doi:

Abstract ( 759 )

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To explore the method of in vitro maturation of Liaoning Cashmere goats oocytes, this experiment was carried out to study effects of cumulus cells on in vitro maturation and parthenogenetic development of oocytes. The oocytes were recovered directly from the oocytes of follicles, ＞2 mm in diameter. Experiment 1, DOs(denuded oocytes) were obtained from COCs freed of whole CCs, and then the oocytes were cultured by the following four ways: (1)COCs were cultured alone;(2) DOs were co-cultured with COCs (DOs(COCs));(3) DOs were co-cultured with CCs (DOs(CCs)); (4) DOs were cultured alone.Experiment 2, the effect of different layers of cumulus cells on the maturative ability and parthenogenetic development of oocytes were evaluated. These oocytes were divided into COCs3,COCs_1-3 and NOs according to the layers of cumulus cells.We found that there was significant difference in maturation and cleavage rates between ooctyes invested by different layers of cumulus cells, and the oocytes enclosed with more layers of cumulus cells, both maturation and cleavage rates were higher than oocytes enclosed with fewer layers of cumulus cells. The DOs(COCs) was better than DOs(CCs), and the NOs was a kind of degenerate ooctyes.Our findings would be helpful to the choice of recipient oocytes for embryonic technologies which will need high quality oocytes.

Study on Melamine Deposition and Depletion in Different Tissues of Laying Ducks

WU Shu-geng;GAO Chun-qi;YUE Hong-yuan;ZHANG Hai-jun;QI Guang-hai

2010, 41(5): 549-556. doi:

Abstract ( 741 )

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The objective of this study was to investigate the deposition and depletion of melamine in different tissues of laying ducks. Four hundred and thirty-two 19-week-old Jinding laying ducks(Body weight:1.66±0.16 kg, egg production:75.15%±3.29%) were randomly divided into 4 groups with 6 replicates of 18 birds each. Each group was fed a corn-soybean meal diet supplemented with 0, 5, 50 and 100 mg·kg^-1 melamine respectively for 21 d. The melamine concentration in the plasma, kidney, liver and breast muscle were determined on day 4,7,14 and 21 d. After switching the basal diet for 8,24,48 and 96 h the melamine content were determined in the plasma, kidney, liver and breast muscle samples. After adding melamine to the dietary for 21 days, the average melamine in different tissues of 50 and 100 mg·kg^-1 melamine treatment groups were significantly higher than that of 0, 5 mg·kg^-1 melamine treatment groups(P<0.05), there was no significant difference between 0 and 5 mg·kg-1 groups（P>0.05）. The melamine concentration in tissues of 100 mg·kg^-1 group decreased progressively by the order of plasma> kidney>liver>breast muscle. Higher dietary melamine concentration requires a longer time to eliminate from the tissues. After withdrawing melamine diets for 96 h, the melamine concentrations in different tissues were decreased significantly, but the melamine in different tissues can not be completely eliminated in 96 h. Cyanuric acid had not been detected in the tissues of laying ducks. The melamine concentration in the different tissues were increased with the levels of melamine in the diet, and showed doseresponse relationship. Melamine needs a long duration, which can be demonstrated by the incomplete elimination of melamine in the tissue within 96 h. Melamine concentrations in plasma and kidney were higher than that in liver and breast muscle. The study indicates that plasma and kidney can be used as the indicator for the residue of melamine. The depletion time for melamine in different tissues should be longer than 96 h.

Effects of Seasons on the Quality and Nutritional Values of Wholeplant Corn Silage

YAN Gui-long;CAO Chun-mei;DIAO Qi-yu;DENG Kai-dong

2010, 41(5): 557-563. doi:

Abstract ( 640 )

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The effects of seasons on the quality and nutritional values of whole-plant corn silage in silos were evaluated in this study. The silage were sampled 5, 10, 20 or 40 cm behind the removal surface of whole-plant corn silage in aboveground silos in winter, spring and summer, and analysed for the quality, chemical components and in vitro DM digestibility. The results showed that the population of microorganisms in the silage was not affected significantly in winter and spring (P>0.05), but the fungal population in the silage taken 5 or 10 cm behind the removal surface was higher in summer than that in spring or winter (P<0.01). After decreasing to 3.76, the pH value of the whole-plant corn silage continued to drop for a long time from the winter to next spring and summer during which the nutrients unavailable to microorganisms such as NDF and ADF, increased gradually and those available nutrients such as total sugars, decreased proportionally, and in vitro DM digestibility decreased gradually, too. Therefore, nutritional values of whole-plant corn silage decreased gradually during storage in silos, and the daily removal rate of the silage should be over 10 cm in summer.

Toxicity of Oxidized Fish Oil to Broiler Breeder Hens and Injury on Reproduction and Intestinal Morphology of the Offspring

AN Sheng-ying;GUO Yu-ming;WANG Zhong;Zhang Li-bin;MA Shou-dong;Bun Sidoeun

2010, 41(5): 564-569. doi:

Abstract ( 1010 )

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This experiment was conducted to investigate effects of oxidized oil and antioxidant supplementation in breeder hen diet on reproduction performance of the breeders and maternal effects to the offspring. A total of 200 Cobb-Avian 48, 29 weeks old broiler breeder hens were assigned randomly to 5 groups with 5 replicates of 8 birds. Each group was fed one of the following diets; 2% fish oil (FO), 2% oxidized oil（OFO）, 2% OFO+100 mg·kg^-1 VE, 2% OFO+198 mg·kg^-1 EMQ, 2%OFO+100 mg·kg^-1VE+198 mg·kg^-1 EMQ, the progeny broilers were fed the same diet. The results showed that: (1) Inclusion of OFO in the breeder hens′ diet reduced laying percentage（P<0.05） and egg production（P<0.05）of the breeders; (2) OFO in the breeder hens′ diet reduced neonatal offspring chick weight of the breeders（P<0.05）; (3) Offspring chicks from the dam feeding oxidized oil had shorter jejunum villus height (P<0.01) and thinner jejunum villus width (P<0.01); (4) OFO in the breeder hens′ diet reduced Body weight gain of 0-7 d (P<0.01), body weight at 7 d (P<0.05), feed intake of 7-14 d (P<0.01) and body weight at 14 d (P<0.01) of the offspring broilers. These data indicated that oxidized oil had obvious toxicity to the breeder hens, not only reduced the reproduction performance of the breeder hens, but also decreased the neonatal offspring chick body weight, injuried the morphology of the jejunum of offspring chicks, resulted in decreased offspring performance.

Effects of Botanical Activity Flour of Garlic Bacterium Substance onPerformance and Hormonal Secretion of Yangzhou Geese

SUN Zhen-ping;ZHAO Xue-min;MENG Yan;GUO Xi;BAI Dong-xing;REN Ying;ZHANG Yang-jun;YANG Sheng-rong

2010, 41(5): 570-575. doi:

Abstract ( 709 )

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In order to study the effects of Botanical activity flour of garlic bacterium substance (GBS) with four doses on performance and hormone secretion of Yangzhou geese, and provide theoretical basis and application consult for the development and utilization, 240 one-day-old Yangzhou geese were allocated to five groups,including control group and trial group 1,2,3,4. The control group was fed with basical diets，each trial group was fed with basical diets added with 0.25,0.50,1.00,2.00 g·kg^-1 GBS,respectively. The results showed that, compared with control group, the body weight gain of 1-60-day-old trial groups increased by 5.91% (P<0.05), 8.18% (P<0.01),13.64%(P<0.01),13.64%(P<0.01); the feed intake in trial group 4 increased by 8.79% (P<0.01); the feed conversion ratio in trial group 2,3 decreased by 7.55%(P<0.05), 9.37%(P<0.01). Crude protein of brisket in trial group 2,4 increased by 22.88%(P<0.05), 17.81%(P<0.05); Crude fat of brisket in each trial group decreased by 22.82%(P<0.05), 31.21%(P<0.05), 44.63%(P<0.05),33.22%(P<0.05). Serum T3 in trial group 3,4 increased by 11.76%(P<0.01),14.38%(P<0.01); serum T4 in each trial group increased by 7.75%(P<0.01),14.06%(P<0.01),14.30% (P<0.01), 66.52%(P<0.01); serum Glu in trial group 1,3,4 decreased by 14.78%(P<0.01), 14.45% (P<0.01),16.05%(P<0.01). The average level of Ins after feeding in 3 h trial group 2，3，4 decreased by 14.85%(P<0.01), 12.54%(P<0.01),23.27% (P<0.01); the average level of serum glucose in trial group 1,3,4 decreased by 7.96%(P<0.01),6.80%(P<0.01),11.30% (P<0.01). It had a encourage effect on performance and hormone secretion of Yangzhou geese by supplemented with GBS on their Goose die, and four doses in this trial, the effects of fed basical diets added with GBS 2.00 g·kg^-1 is the best.

Primary Selection of MicroRNA Acting on Classical Swine FeverVirus in Swine Umbilical Vein Endothelial Cells

HOU Bo;ZHANG Yan-ming;ZHU Xiao-juan;KANG Kai;DAI Chen;TANG Qing-hai

2010, 41(5): 576-580. doi:

Abstract ( 701 )

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The aim of the study is to find out the microRNAs that acted on classical swine fever virus (CSFV) in swine umbilical vein endothelial cells (SUVECs). CSFV 3′ and 5′untranslated region (UTR) were cloned, and then were inserted into psiCHECH^TM-2 plasmid that carrying Firefly and Renilla luciferases reporter genes; the microRNAs that acted with CSFV were forecasted through bioinformatics analysis; then the recombinant plasmids and inhibitors of predicted microRNA were cotransfected into SUVECs. The activity of Firefly and Renilla luciferases was detected by Luminometer. The PCR products of CSFV 5′UTR（373 bp） and 3′UTR (252 bp) were detected by 10 g·L^-1 agarose electrophoresis consistent with predicted size, from which four predicted microRNAs (ssc-miR-let7c, ssc-miR-106a, ssc-miR-18, ssc-miR-139) were selected. The CSFV 3′UTR is the important action site of microRNA in SUVECs. The four microRNAs mentioned above had different inhibitory effect on CSFV 3′UTR, of which the ssc-miR-18 play a more important role.

Study of TNF-α Mediated Signal Pathway Involved in IFN-γ-Promoted Apoptosis of THP-1 Cells during Infection with Mycobacterium bovis

ZHANG Jiao-er;WANG Zhi-yuan;HUANG Ying;WANG Yang;YIN Xiao-min;ZHOU Xiang-mei;ZHAO De-ming

2010, 41(5): 581-586. doi:

Abstract ( 1004 )

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This experiment was conducted to study the promoting effect of IFN-γ on apoptosis of THP-1 cells infected with Mycobacterium bovis, and the role of TNF-α and its relevant signal molecules during cell apoptosis. IFN-γ (15 ng·mL^-1) was added to PMA differentiated THP-1 cells infected with M. bovis-BJ at different dose of MOI (10∶1, 20∶1), the percentage of apoptotic cells were analyzed by flow cytometry at 12, 24, 36, 48 and 72 h post infection. The results showed that IFN-γ promoted apoptosis of THP-1 cells infected with M. bovis was in a time-dependent manner. As incidence of apoptotic M. bovis–infected THP-1 cells treated with IFN-γ increased, the number of viable bacilli decreased. Anti-TNF-α monoclonal antibodies（3.0 μg·mL^-1, Mab）were added to THP-1 cells treated with IFN-γ and M. bovis (MOI, 10∶1), and the percentage of apoptotic cells and caspase-3, 8, 9 activity were determined at 36 h post infection. The results showed that the addition of anti-TNF-α Mab inhibited apoptosis of THP-1 cells treated with IFN-γ and M. bovis. A significant difference was found in the THP-1 cells treated with IFN-γ and M. bovis in the presence or absence of anti-TNF-α Mab (P<0.05), and that caspase-3 and caspase-8 were activated via a TNF-α dependent manner. THP-1 cells treated with IFN-γ and M. bovis-BJ were incubated for 36 hours in the presence of the following reagents: JNK inhibitor I or NEMO-Binding Domain (NBD) Binding Peptide alone, or the two inhibitors combined. No significant difference of THP-1 cells apoptosis in the presence or absence of the two inhibitors were observed (P＞0.05), indicating that JNK and NF-κB signal pathways are unlikely to be activated. This study elucidated that TNF-α associated in signal pathway of THP-1 cell apoptosis promoted by IFN-γ during early infection with Mycobacterium bovis.

Construction and Characterization of a Salmonella Choleraesuis C78-1 Δcrp Deletion Mutant

YU Chuan;CHENG Xiang-chao;ZHAO Zhan-qin;ZHANG Chun-jie;LI Yin-ju;WU Ting-cai

2010, 41(5): 587-593. doi:

Abstract ( 756 )

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In this study, a Δcrp mutant of Salmonella Choleraesuis C78-1 was constructed by the allelic exchange introduced by the transduction of the pRE112 suicide plasmid and characteristics of the mutant were also determined. Firstly, the upstream and downstream fragements of crp gene were amplified from Salmonella Choleraesuis C78-1 genome and then successively subcloned into suicide plasmid pRE112 to construct the recombinant suicide vector pREΔcrp with 320 bp-deleted crp fragment. Then, the C78-1 Δcrp mutant was constructed through the two-step method introduced by the transduction of recombinant suicide plasmid. The O and H antigens of the mutant was 6,7:C:1,5, identical to the parent C78-1 strain. The mutant was stabile with the recombinant Δcrp gene in vitro. However, fermentation patterns and growth rate of the mutant were differed from that of the parent C78-1 strain, obviously. The mouse lethal test showed that the virulence of the mutant was 750 times lower than C78-1. These results showed that the C78-1 Δcrp mutant was constructed successfully. It is likely that this Δcrp mutant could be adapted to develop attenuated Salmonella vaccine.

Detection of the Streptococcus suis of Clinical Healthy Pigs in Chongqing

WANG Kai-cheng;XIONG Zhong-liang;SHANG Yan-ming;HUANG Bao-xu;TANG Ming;TAN Shi-jun;ZHANG Dong;CAI Zhen-hong;FAN Wei-xing

2010, 41(5): 594-599. doi:

Abstract ( 1019 )

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This research was conducted to investigate the Streptococcus suis carrier state of pigs in Chongqing and reveal the importance of the state in public health. One thousand three hundred and sixty palatine tonsils of clinical healthy slaughter pigs were collected from 17 abattoirs in different district of Chongqing. Streptococcus suis was isolated from the samples. The serotype 1, 2, 7, 9, 14 and 1/2 was identified, the virulence-associated genes profile and multilocus sequence typing scheme of which was analyzed. Two hundred and twenty-five strains of Streptococcus suis were isolated from the samples, and the isolation rate was 16.54%. Of the 225 isolates recovered from slaughter pigs, 4 Streptococcus suis type 2 isolates, 3 Streptococcus suis type 7 isolates, 3 Streptococcus suis type 9 isolates were identified and 1 Streptococcus suis type 1/2 isolate was detected. Virulence-associated gene profiling analysis showed that majority isolates(10/11) were defective of some virulence-associated gene; but the isolates possessing all known virulence-associated gene also existed; the short type mrp phenotype Streptococcus suis type 1/2(1 isolate) and type 7(2 isolates) isolates were firstly identified in China. It is obvious that the carrier state of clinical healthy pigs bring potential infection jeopardize to butcher; the prevalent isolates are little different from the other country.

Cell Growth, Apoptosis and the mRNA Transcription of Heat Shock Protein:Effects of Heat Stress on Bovine Mammary Epithelial Cells

ZHOU Zhen-feng;CUI Rui-lian;WANG Jia-qi;WEI Hong-yang;BU Deng-pan;HU Han;;ZHOU Ling-yun

2010, 41(5): 600-607. doi:

Abstract ( 712 )

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The experiment was conducted to study the effects of heat stress on bovine mammary epithelial cells in vitro. Bovine mammary epithelial cells were exposed to 42 ℃ as the heat stress model and 38 ℃ as control, then cell growth, apoptosis and heat shock protein expression were detected by trypan blue dying, flow cytometry FCM , RT-qPCR and ELISA. The results showed as follows: cell growth stagnated at 42 ℃; the cell number decreased markedly (P<0.05) than control 38 ℃ at the second day, and decreased extremely significantly (P<0.01) from the third to the seventh day; the apoptosis rate was the highest at the 3rd hour after heat treatment; the mRNA expression level of heat shock protein 27, 70, 90 (HSP27,HSP70,HSP90) and heat shock factor-1 (HSF-1) were 2.72, 7.48, 2.69 and 2.71 folds as control group, separately, which were all significantly higher than control(P<0.01). In conclusion, heat stress could inhibit cell natural growth, induce apoptosis, and upregulate the mRNA of HSPs.

Effect of Polysaccharide, Flavonoids and Saponins of Polysavone onExpression of Mouse Beta-defensins in RAW264.7 Cells

YANG Jiang-tao;DONG Xiao-fang;TONG Jian-ming;LIU Qing-xue;ZHANG Qi;WU Ying-ying

2010, 41(5): 608-614. doi:

Abstract ( 681 )

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To investigate the effect of polysaccharide, flavonoids and saponins of Polysavone on the expression of mouse beta-defensins in RAW264.7 cells, RAW264.7 cells were cultured with Polysavone polysaccharide at 30,60,90,150 μg·mL^-1, Polysavone flavonoids at 10,20,30,50 μg·mL^-1, Polysavone saponins at 10,20,30,50 μg·mL^-1 , respectively. The mRNA expression of mouse beta-defensin (mBD1-3) were detected. The results showed that the mBD2 and mBD3 mRNA expression could be improved by Polysavone polysaccharide, especially the treatment with 30 μg·mL^-1, but there was no effect on the mRNA expression of mBD1. The mBD1 mRNA expression could be improved by Polysavone flavonoids, especially the treatment with 10 μg·mL^-1, the mBD2 and mBD3 mRNA expression could be inhibited. The mBD1 mRNA expression could be improved by Polysavone saponins, the mBD3 mRNA expression could be inhibited, but Polysavone saponins had no effects on the mRNA expression of mBD2. The results showed the different effects of polysaccharide,flavonoids and saponins of Polysavone on the mRNA expression of mBD1,mBD2 and mBD3. The Polysavone polysaccharide may enhance the function of adaptive immunity. The flavonoids and saponins of Polysavone may enhance the function of innate immunity.

The Distribution of IgA and IgG Secreting Cells in the GastrointestinalTract of the Weaning Piglets

CHE Chuan-yan;YANG Qian

2010, 41(5): 615-620. doi:

Abstract ( 666 )

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This experiment was conducted to study the distribution characteristics and patterns of IgA and IgG secreting cells in the different sites of the gastrointestinal tract of the weaning piglets. Tissue samples of gastric fundus, gastric body, gastric pylorus, duodenum, jejunum, ileum and colon were collected from eight piglets weaned for 24 days. These tissue samples were studied by immunohistochemistry methods for detecting the changes of IgA and IgG secreting cells in the gastrointestinal tract. The results showed that the distribution of IgA and IgG secreting cells along the gastrointestinal tract had similar distribution tendency and patterns. IgA and IgG secreting cells were mainly distributed in the lamina propria, and the numbers of the two sorts of secreting cells were firstly increasing and then decreasing along the gastrointestinal tract. In addition, IgA and IgG secreting cells were mostly distributed in the gastric pylorus and the small intestine. These results demonstrated that the gastric pylorus and the anterior segment of the small intestine of piglets may be the important sites of playing the mucosal immune reactions and protecting against the pathogenic microorganism.

Screening and Identification of Swainsonine-Secreting Fungal

MA Yao;LU Hao;ZHAO Bao-yu;LAI Hang-xian;LI Rong;WANG Zhan-xin;CHEN Ji-ping;LU Wei;RONG Jie

2010, 41(5): 621-629. doi:

Abstract ( 1063 )

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The objective of this study was to explore whether the fungus of swainsonine-secret exist in locoweeds and rhizosphere soil, identify genus of those fungus and determinate their swainsonine yield in Qinghai Province and Inner Mongolia Autonomous Region. Fungus was isolated from the locoweed and soil samples, and swainsonine was detected by thin layer chromatography and gas chromatography for isolated fungus. Two strains of endophytic fungi produced the alkaloid swainsonine were isolated and obtained from locoweeds and rhizosphere soil, and named FS-5 and EFG-7, and SW yield is 0.773 and 0.11 mg·g^-1, respectively. Morphological evidence and sequence analysis of the ITS region suggest that FS5 is most closely related to Schizophyllum sp, and EFG-7 is Fusarium tricinctum. The results showed that the fungus of swainsonine-producing exist in locoweeds and rhizosphere soil in Qinghai Province.

Effect of Different Cultivars Raw Soybean on the Growth Performance andPlasma Physiology and Biochemistry Parameters in Rats

PAN Hong-bin;GU Chun-mei;SUN Ze-wei;QIN Gui-xin

2010, 41(5): 630-634. doi:

Abstract ( 1057 )

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The objective of this study was to investigate the antinutritional effect of five different China cultivars raw soybean in rats. The content of soybean trypsin inhibitor(PI) and lectin was measured in five different cultivars raw soybean: Jinlinxiaoli6, Jlin30,Jilin45,Jinong7 and Fengjiao7607. Fifty rats aged 50±2 days were randomly divided into five groups, with each group involved 10 replicates. The five groups rats were fed on the diets containing raw soybean cultivars: Jinlinxiaoli6, Jlin30,Jilin45,Jinong7 and Fengjiao7607, respectively. The whole experiment period lasted for 30 days, the growth performance of individual rat was calculated. All rats were collected blood by jugular vein before slaughtered on 30 d, physiology and biochemistry parameters of the plasma were detected. The results showed that the content of PI of Jilin30,Jilinxiaoli6, Fengjiao7607 and Jinong7 cultivars raw soybean were significant high compared to Jilin45 (P<0.05),the content of lectin of Jilinxiaoli6 cultivar raw soybean was significant high compared to Jinong7,Fengjiao7607, Jilin45 and Jilin30 (P<0.01), The total bilirubin (TBIL) of rats fed on the diets containing Jilinxiaoli6 cultivar raw soybean was significant high compared to Jilin45 and Fengjiao7607 (P<0.05). The results showed that effect of antinutritional factors in feeding rats with different cultivars raw soybean diets are Jinlinxiaoli6＞Jilin30＞Fengjiao7607＞Jilin45＞Jinong7.

Molecular Evolution Analysis for gp85 Gene of Subgroup J-Avian LeukosisViruses Strains from Hyline Egg-type Chickens in Different Areas

DAI Yang;YANG Qi-feng;WANG Bo;LIU Shao-qiong;WANG Xiu-zhen;CHAI Jia-qian;CUI Zhizhong;SUN Shu-hong

2010, 41(5): 635-638. doi:

Abstract ( 668 )

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By the homology analysis for gp85 genes of 5 strains of ALV-J isolated from Hyline laying hens, the molecular evolution of ALV-J was studied in the present study. The 5 strains of ALV-J were obtained from Beijing, Shanxi and Shandong. The gp85 genes were cloned by PCR and the sequence was measured and compared with 14 published reference ALV-J strains. The homology analysis showed that the 5 isolated strains of ALV-J shared 96.6% (96.4%-96.8%) and 89.6% (89.3%-89.9%) homology with the HPRS-103 strain isolated from white meat-type broiler and SD07LK1 strain isolated from egg-type chickens. The homology of the 5 strains was exceeded 98.1% (98.1%-100%). The result indicated that ALV-J strains from Hyline egg-type chickens may have the same origin, the white meat-type broiler chickens imported abroad.

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