Long non-coding RNAs(lncRNAs) is a group of RNAs, which are longer than 200 nucleotides without containing functional open reading frames and cannot encode proteins. The studies indicate that lncRNAs can participate in the epigenetic modification. The most genome in mammals and other eukaryotes is transcribed in a developmentally regulated manner to produce large numbers of long non-coding RNAs. lncRNA can regulate DNA methylation, histone modifications, chromatin remodeling and other RNA interference through many ways, and the expression and role of lncRNA can be regulated by DNA methylation and chromatin remodeling. This article will review the lncRNAs function and its important role in gene regulation and medicine.