大车前苷抑制猪肺炎支原体诱导的炎性反应的作用初探

doi:10.11843/j.issn.0366-6964.2025.04.042

Abstract

Abstract:

Mycoplasma hyopneumoniae (Mhp) colonizes to respiratory epithelial cells, causing the inflammatory injury of trachea, bronchus and lungs in pigs, which reduced the feed conversion rate and daily weight gain of pigs, resulting in serious economic losses to the swine industry. plantamajoside (PMS), as a commonly used anti-inflammatory traditional Chinese medicine, its role in inhibiting the inflammatory response caused by Mhp infection is unknown. The aim of this study was to investigate the effect and related mechanisms of PMS on inflammatory response induced by Mhp in vitro and in vivo. Mhp infected PK-15 cells and mice were used as infection models, respectively. First, PK-15 cells were treated with different concentrations of PMS to determine the maximum concentration of PMS that had no effect on cell morphology and viability. This highest concentration of PMS was then applied to PK-15 cells which infected with Mhp. The mRNA expression of tumor necrosisfactor-alpha (TNF-α), interleukin-6 (IL-6) and other inflammatory factors in PK-15 cells was detected by RT-qPCR. The results showed that the mRNA levels of IL-6, TNF-α, NF-κB and IκBα were significantly increased in PK-15 cells which infected with Mhp compared with the control group, while PMS could reduce the effect. Western blot and immunofluorescence were used to further analysis and the results showed that PMS could inhibited the phosphorylation of NF-κB P65 and IκBα, and inhibited the nuclear translocation of NF-κB P65, which induced by Mhp. Related studies on mouse models have found that PMS can improve the lung lesions, reduce the inflammatory cells of lung which induced by Mhp. At the same time, PMS can significantly reduce the content of TNF-α in serum. These results suggest that PMS may ameliorate Mhp-induced inflammatory response through NF-κB pathway, which provides important theoretical basis for novel prevention and treatment of Mhp infection.

Key words: plantamajoside(PMS), Mesomycoplasma hyopneumoniae (Mhp), pneumonia, NF-κB

CLC Number:

WANG Jiying, XIANG Shuhan, LI Zhiqiang, LI Shiyang, ZHANG Lei, XIE Qingyun, XIONG Qiyan, SHAO Guoqing, FENG Zhixin, YU Yanfei. Preliminary Study on the Inhibitory Effect of Plantamajoside on Inflammatory Response Induced by Mesomycoplasma hyopneumoniae[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(4): 1958-1968.

Figures/Tables 9

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引物名称 Name	正向引物序列(5′→3′) Sequences of forward primer	反向引物序列(5′→3′) Sequences of reverse primer
GAPDH	CCCTGAGACACGATGGTGAA	GGAGGTCAATGAAGGGGTCA
IL-6	ATGGCAGAAAAAGACGGATG	GTGGTGGCTTTGTCTGGATT
TNF-α	CCCCTCTGAAAAAGACACCA	TCGAAGTGCAGTAGGCAGAA
IL-1β	CAGCTGCAAATCTCTCACCA	TCTTCATCGGCTTCTCCACT
IκBα	CCTGGTGTCGCTCTTGTTGA	GCTGCTGTATCCGAGTGCT
NF-κB	TGGTGGGAAAACACGGAGAG	ATGCCTCTGTCATTCGTGCT

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Preliminary Study on the Inhibitory Effect of Plantamajoside on Inflammatory Response Induced by Mesomycoplasma hyopneumoniae

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