染料木素对牛精液冷冻保存效果的影响

doi:10.11843/j.issn.0366-6964.2025.02.021

Abstract

Abstract:

The study aimed to investigate the effect of genistein (GEN) added to the semen extender on the cryopreservation of semen from cattle, and to provide a theoretical basis for optimizing the semen extender of cattle and improving the sperm quality of cattle after freezing and thawing. In this study, the fresh semen of 6 health Qinchuan bulls aged 3-5 years old, weighing (586±20) kg were collected, and the semen that passed the test was mixed, diluted with different concentrations of genistein for cryopreservation. According to the different concentrations of GEN in the semen extender, this study was divided into 5 groups, 0 μg·mL^-1 GEN group (control group), 30 μg·mL^-1 GEN group, 60 μg·mL^-1 GEN group, 90 μg·mL^-1 GEN group, and 120 μg·mL^-1 GEN group, with 3 replicates in each group. After thawing, bovine automatic sperm quality analyzer was used to measure the motility performance of sperm, hypotonic swelling detection method and peanut lectin fluorescent labeling method were used to detect sperm acrosome integrity and plasma membrane integrity, and the kits were used to detect mitochondrial membrane potential (MMP), antioxidant performance, malondialdehyde (MDA) content and reactive oxygen species (ROS) level. Compared with the control group, the forward motility sperm rate of the 30, 60 and 90 μg·mL^-1 GEN groups was significantly increased (P < 0.05), and the acrosome integrity of sperm in the 30 and 60 μg·mL^-1 GEN groups was significantly increased (P < 0.05). The ROS level in the 60 μg·mL^-1 GEN group was significantly lower than that in the other groups (P < 0.05). Compared with the control group, the contents of T-AOC and SOD were significantly increased in the GEN group (P < 0.05), the MDA content was significantly decreased in the 30, 60 and 90 μg·mL^-1 GEN groups (P < 0.05), and the GSH-px content was significantly increased in the 60, 90 μg·mL^-1 GEN groups (P < 0.05), the CAT content was significantly increased in the 60, 120 μg·mL^-1 GEN groups (P < 0.05). The MMP of the 60 μg·mL^-1 GEN group was significantly higher than that of the other groups (P < 0.05). The results showed that the supplementation of the semen extender with genistein could improve the antioxidant performance of sperm in bovine semen, inhibit the oxidative stress of sperm during cryopreservation, alleviate the freeze-thaw damage of sperm acrosomes and mitochondria, and improve the motility performance of sperm after thawing, so as to improve the quality of bull semen after freezing-thawing, and the concentration of genistein was 60 μg·mL^-1.

Key words: genistein, cryopreservation of bovine semen, mitochondrial membrane potential, antioxidant performance

CLC Number:

S823.3

LIANG Entang, LI Huaxuan, CHEN Shuaicheng, LI Guo, SUN Gege, ZAN Linsen. Effect of Genistein on Semen Cryopreservation of Bull[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(2): 700-710.

Figures/Tables 8

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项目 Item	染料木素浓度/(μg·mL^-1)GEN					P-value
项目 Item	0	30	60	90	120	Treat	Linear	Quadratic
活动精子率/% Motility	57.20±2.76	60.40±0.40	63.37±0.52	58.83±1.20	57.87±1.67	0.05	0.96	0.02
前向运动精子率/% Promotility	31.33±1.81^b	44.57±1.42^a	48.17±0.47^a	43.50±2.26^a	40.87±4.34^ab	< 0.01	0.04	< 0.01
前向运动精子平均速率/ (μm·s^-1) Velocity	33.33±1.76	38.00±0.58	39.00±0.58	38.33±0.88	36.67±1.86	0.26	0.11	0.01

项目 Item	染料木素浓度/(μg·mL^-1)GEN					P-value
项目 Item	0	30	60	90	120	Treat	Linear	Quadratic
质膜完整性/% Membrane integrity	57.20±1.16	60.06±0.56	63.28±1.35	58.39±1.83	57.93±1.89	0.18	0.98	0.02

项目 Item	染料木素浓度(μg·mL^-1) GEN					P-value
项目 Item	0	30	60	90	120	Treat	Linear	Quadratic
顶体完整性/% Acrosome integrity	54.61±0.61^c	59.23±0.81^b	64.58±0.42^a	57.63±0.48^bc	55.32±1.00^c	< 0.01	0.94	< 0.01

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Effect of Genistein on Semen Cryopreservation of Bull

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