嵌合流行毒株G-H环基因重组口蹄疫病毒的拯救及其免疫原性分析

doi:10.11843/j.issn.0366-6964.2024.11.037

Abstract

Abstract:

In order to develop FMD vaccine candidate strain that could effectively prevent and control recently epidemic strains belonging to three topotypes of serotype O FMDV, we constructed recombinant full-length clone containing the structural protein genes belonging to three topotypes of type O FMDV by gene replacement based on FMDV reverse genetic manipulation technique. The recombinant FMDV was rescued after transfection the full-length plasmid into BSR/T7 cells expressing T7 RNA polymerase. The effect of gene reconstruction on the biological characteristics of the recombinant virus was analyzed, pigs and cattle were vaccinated with the vaccines prepared from the recombinant virus and parental virus, the potential of the recombinant virus as a vaccine candidate strain for type O FMD was preliminarily studied by in vitro neutralization test. The results showed that the replacement of G-H loop gene of structural protein VP1 of FMD epidemic strain did not significantly affect the plaque phenotype and replication ability of the recombinant virus, but different G-H has obvious effect on production the cross-neutralizing antibody in pigs, and has little effect on production the cross-neutralizing antibody in cattle, indicating that FMDV G-H loop is the immune dominance epitope for pigs. This study will provide important reference for the design of FMDV vaccine in future.

Key words: G-H loop, recombinant foot-and-mouth disease viruse, rescue, immunogenicity analysis

CLC Number:

S852.659.6

Pinghua LI, Shulun HUANG, Keqiang ZHANG, Feng LIU, Pu SUN, Dong LI, Huifang BAO, Yimei CAO, Xingwen BAI, Xueqing MA, Kun LI, Hong YUAN, Zaixin LIU, Zengjun LU. Rescue and Immunogenicity Analysis of Recombinant Foot-and-Mouth Disease Virus with the Chimera of G-H Loop Gene of the Epidemic Strain[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(11): 5222-5229.

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Rescue and Immunogenicity Analysis of Recombinant Foot-and-Mouth Disease Virus with the Chimera of G-H Loop Gene of the Epidemic Strain

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