Table 1

Common field of view Area of the eyepiece under a 40×objective lens"

Table 2

Experimental groups of mice inoculated with PRU tachyzoites"

Table 3

Sequence of nested PCR primers"

Table 4

Nested PCR procedures"

Table 5

Results of serum antibodies to Toxoplasma gondii detected by ELISA in living mice"

Fig. 1

Survival curves of each strain of mice"

Fig. 2

The death time of different strains of mice inoculated with different number of tachyzoites *.P < 0.05, **.P < 0.01, ***.P < 0.001, ****.P < 0.000 1, ns.P>0.05"

Fig. 3

Nested PCR detection of cysts in various tissues M. DL 2000 DNA marker; 1. Myocardial; 2. Liver; 3. Spleen; 4. Lung; 5. Kidney; 6. Brain; 7. Pectoral muscle; 8. Thigh muscle; N. Negative control (Nested PCR external primer product on the left and internal primer product on the right in each group)"

Fig. 4

cysts of different diameters in the brain of mice (scale bar=100 μm)"

Fig. 5

Cysts of PRU strain Left: Cysts purified by lymphocyte isolation solution; Right: Cyst morphology after staining with Schiff acid. Scale =50 μm"

Fig. 6

The number of cysts in the brain tissue of surviving mice in each experimental group"

Table 6

Comparison of cysts preserved at 4 ℃ for 40 days with fresh cysts on the infection of mice"

Table 7

Monitoring time points and results of cysts produced after tachyzoite infection in C57BL/6 mice"

Fig. 7

Morphology of early cysts A. Immature cysts in unpurified brain tissue (scale bar=50 μm); B. Irregularly shaped cysts(scale bar=20 μm); C. Cyst wall incomplete cyst(scale bar=20 μm); D. Small diameter cyst (scale bar=20 μm)"