动物肠类器官培养技术

doi:10.11843/j.issn.0366-6964.2023.07.008

Abstract

Abstract: Organoids are defined as three-dimensional (3D) multicellular self-assembled tissue structures in vitro, which could simulate corresponding organs in vivo in terms of cell types, structural architecture and function. They have obvious tissue specificity and can be used as a novel experimental model in vitro. With the development of technology, organoids have been applied in many fields including organ morphogenesis, organ function, organ repair and transplantation, disease diagnosis, drugs screening, etc. Currently, other than murine intestinal organoid culture, only few studies on animal intestinal organoid culture were reported. However, other animals, especially domestic animals, still suffer from infectious and non-infectious intestinal diseases that urgently need a new study model for pathogenesis or new therapy. Therefore, this research progress focused on animal intestinal organoids culture in the last ten years with the advantages and the disadvantages of it were being summarized. This review may provide new ideas for the related research of animal intestinal organoids.

Key words: animal intestinal organoid, 3D culture

CLC Number:

Q813.13

CHEN Aolei, HUANG Deru, AN Yafei, LI Shoujun. Animal Intestinal Organoids Culture[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2743-2750.

References 60

[1]	MENESES A M C, SCHNEEBERGER K, KRUITWAGEN H S, et al. Intestinal organoids-current and future applications[J]. Vet Sci, 2016, 3(4):31.
[2]	BARKER N, VAN ES J H, KUIPERS J, et al. Identification of stem cells in small intestine and colon by marker gene Lgr5[J]. Nature, 2007, 449(7165):1003-1007.
[3]	SATO T, VRIES R G, SNIPPERT H J, et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche[J]. Nature, 2009, 459(7244):262-265.
[4]	CLEVERS H. Modeling development and disease with organoids[J]. Cell, 2016, 165(7):1586-1597.
[5]	SATO T, CLEVERS H. Growing self-organizing mini-guts from a single intestinal stem cell:mechanism and applications[J]. Science, 2013, 340(6137):1190-1194.
[6]	ZHANG Y J, HUANG S B, ZHONG W G, et al. 3D organoids derived from the small intestine:an emerging tool for drug transport research[J]. Acta Pharm Sin B, 2021, 11(7):1697-1707.
[7]	MADELINE A L, JUERGEN A K. Organogenesis in a dish:modeling development and disease using organoid technologies[J]. Science, 2014, 345(6194):1247125.
[8]	YIN Y B, DE JONGE H R, WU X, et al. Mini-gut:a promising model for drug development[J]. Drug Discov Today, 2019, 24(9):1784-1794.
[9]	DATE S, SATO T. Mini-gut organoids:reconstitution of the stem cell niche[J]. Annu Rev Cell Dev Biol, 2015, 31:269-289.
[10]	CLEVERS H C. Organoids:avatars for personalized medicine[J]. Keio J Med, 2019, 68(4):95.
[11]	WANG X, YAMAMOTO Y, WILSON L H, et al. Cloning and variation of ground state intestinal stem cells[J]. Nature, 2015, 522(7555):173-178.
[12]	LI L, FU F, GUO S S, et al. Porcine intestinal enteroids:a new model for studying enteric coronavirus porcine epidemic diarrhea virus infection and the host innate response[J]. J Virol, 2019, 93(5):e01682-18.
[13]	LI X G, ZHU M, CHEN M X, et al. Acute exposure to deoxynivalenol inhibits porcine enteroid activity via suppression of the Wnt/β-catenin pathway[J]. Toxicol Lett, 2019, 305:19-31.
[14]	WANG Z, OCADIZ-RUIZ R, SUNDARESAN S, et al. Isolation of enteric glial cells from the submucosa and lamina propria of the adult mouse[J]. J Vis Exp, 2018(138):57629.
[15]	BAGHDADI M B, KIM T H. Analysis of mouse intestinal organoid culture with conditioned media isolated from mucosal enteric glial cells[J]. STAR Protoc, 2022, 3(2):101351.
[16]	SATO T, STANGE D E, FERRANTE M, et al. Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium[J]. Gastroenterology, 2011, 141(5):1762-1772.
[17]	YILMAZ Ö H, KATAJISTO P, LAMMING D W, et al. mTORC1 in the Paneth cell niche couples intestinal stem-cell function to calorie intake[J]. Nature, 2012, 486(7404):490-495.
[18]	SATO T, VAN ES J H, SNIPPERT H J, et al. Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts[J]. Nature, 2011, 469(7330):415-418.
[19]	GONZALEZ-CORDERO A, KRUCZEK K, NAEEM A, et al. Recapitulation of human retinal development from human pluripotent stem cells generates transplantable populations of cone photoreceptors[J]. Stem Cell Rep, 2017, 9(3):820-837.
[20]	THORNE C A, CHEN I W, SANMAN L E, et al. Enteroid monolayers reveal an autonomous WNT and BMP circuit controlling intestinal epithelial growth and organization[J]. Dev Cell, 2018, 44(5):624-633. e4.
[21]	VAN DER HEE B, LOONEN L M P, TAVERNE N, et al. Optimized procedures for generating an enhanced, near physiological 2D culture system from porcine intestinal organoids[J]. Stem Cell Res, 2018, 28:165-171.
[22]	LI Y, YANG N, CHEN J N, et al. Next-generation porcine intestinal organoids:an apical-out organoid model for swine enteric virus infection and immune response investigations[J]. J Virol, 2020, 94(21):e01006-20.
[23]	POWELL R H, BEHNKE M S. WRN conditioned media is sufficient for in vitro propagation of intestinal organoids from large farm and small companion animals[J]. Biol Open, 2017, 6(5):698-705.
[24]	KRAMER N, PRATSCHER B, MENESES A M C, et al. Generation of differentiating and long-living intestinal organoids reflecting the cellular diversity of canine intestine[J]. Cells, 2020, 9(4):822.
[25]	FUJII M, MATANO M, TOSHIMITSU K, et al. Human intestinal organoids maintain self-renewal capacity and cellular diversity in niche-inspired culture condition[J]. Cell Stem Cell, 2018, 23(6):787-793. e6.
[26]	CHANDRA L, BORCHERDING D C, KINGSBURY D, et al. Derivation of adult canine intestinal organoids for translational research in gastroenterology[J]. BMC Biol, 2019, 17(1):33.
[27]	SAXENA K, BLUTT S E, ETTAYEBI K, et al. Human intestinal enteroids:a new model to study human rotavirus infection, host restriction, and pathophysiology[J]. J Virol, 2016, 90(1):43-56.
	MAHE M M, AIHARA E, SCHUMACHER M A, et al. Establishment of gastrointestinal epithelial organoids[J]. Curr Protoc Mouse Biol, 2013, 3(4):217-240.
[28]	GELBERG H B. Comparative anatomy, physiology, and mechanisms of disease production of the esophagus, stomach, and small intestine[J]. Toxicol Pathol, 2014, 42(1):54-66.
[29]	PIERZCHALSKA M, GRABACKA M, MICHALIK M, et al. Prostaglandin E2 supports growth of chicken embryo intestinal organoids in Matrigel matrix[J]. BioTechniques, 2012, 52(5):307-315.
[30]	ZHAO D, FARNELL M B, KOGUT M H, et al. From crypts to enteroids:establishment and characterization of avian intestinal organoids[J]. Poult Sci, 2022, 101(3):101642.
[31]	WANG Y L, HOU Q H, WU Y Q, et al. Methionine deficiency and its hydroxy analogue influence chicken intestinal 3-dimensional organoid development[J]. Anim Nutr, 2022, 8(1):38-51.
[32]	LI J, LI J Jr, ZHANG S Y, et al. Culture and characterization of chicken small intestinal crypts[J]. Poult Sci, 2018, 97(5):1536-1543.
[33]	PANEK M, GRABACKA M, PIERZCHALSKA M. The formation of intestinal organoids in a hanging drop culture[J]. Cytotechnology, 2018, 70(3):1085-1095.
[34]	SUTTON K M, ORR B, HOPE J, et al. Establishment of bovine 3D enteroid-derived 2D monolayers[J]. Vet Res, 2022, 53(1):15.
[35]	DERRICOTT H, LUU L, FONG W Y, et al. Developing a 3D intestinal epithelium model for livestock species[J]. Cell Tissue Res, 2019, 375(2):409-424.
[36]	STEWART A S, FREUND J M, GONZALEZ L M. Advanced three-dimensional culture of equine intestinal epithelial stem cells[J]. Equine Vet J, 2018, 50(2):241-248.
[37]	MUSSARD E, POUZET C, HELIES V, et al. Culture of rabbit caecum organoids by reconstituting the intestinal stem cell niche in vitro with pharmacological inhibitors or L-WRN conditioned medium[J]. Stem Cell Res, 2020, 48:101980.
[38]	BEAUMONT M, BLANC F, CHERBUY C, et al. Intestinal organoids in farm animals[J]. Vet Res, 2021, 52(1):33.
[39]	GONG S Y, ZHENG J C, ZHANG J J, et al. Taxifolin ameliorates lipopolysaccharide-induced intestinal epithelial barrier dysfunction via attenuating NF-kappa B/MLCK pathway in a Caco-2 cell monolayer model[J]. Food Res Int, 2022, 158:111502.
[40]	LIU X H, LI X R, GAO X M, et al. Comparison of the permeability between conjugated estrogens and atenolol in rat in situ single-pass intestinal perfusions model and in Caco-2 cell monolayers[J]. J Drug Delivery Sci Technol, 2022, 68:102786.
[41]	SUN D X, YU L X, HUSSAIN M A, et al. In vitro testing of drug absorption for drug ‘developability’ assessment:forming an interface between in vitro preclinical data and clinical outcome[J]. Curr Opin Drug Discov Devel, 2004, 7(1):75-85.
[42]	MOCHEL J P, JERGENS A E, KINGSBURY D, et al. Intestinal stem cells to advance drug development, precision, and regenerative medicine:a paradigm shift in translational research[J]. AAPS J, 2018, 20(1):17.
[43]	DOU Y T, PIZARRO T, ZHOU L. Organoids as a model system for studying notch signaling in intestinal epithelial homeostasis and intestinal cancer[J]. Am J Pathol, 2022, 192(10):1347-1357.
[44]	TAKAHASHI Y, NOGUCHI M, INOUE Y, et al. Organoid-derived intestinal epithelial cells are a suitable model for preclinical toxicology and pharmacokinetic studies[J]. iScience, 2022, 25(7):104542.
[45]	MARTINEZ-SILGADO A, YOUSEF YENGEJ F A, PUSCHHOF J, et al. Differentiation and CRISPR-Cas9-mediated genetic engineering of human intestinal organoids[J]. STAR Protoc, 2022, 3(3):101639.
[46]	LI M, IZPISUA B J. Organoids-preclinical models of human disease[J]. N Engl J Med, 2019, 380(6):569-579.
[47]	SEEGER B. Farm animal-derived models of the intestinal epithelium:recent advances and future applications of intestinal organoids[J]. Altern Lab Anim, 2020, 48(5-6):215-233.
[48]	KRUITWAGEN H S, OOSTERHOFF L A, VERNOOIJ I G W H, et al. Long-term adult feline liver organoid cultures for disease modeling of hepatic steatosis[J]. Stem Cell Rep, 2017, 8(4):822-830.
[49]	SUZUKI K, KUWABARA K, TAKAHASHI J, et al. 598-Single-cell level analysis of organoids derived from CD patients reveals disease-status related modifications of small intestinal stem cells[J]. Gastroenterology, 2018, 154(S6):S-127.
[50]	LIU Y, CHEN Y G. 2D- and 3D-based intestinal stem cell cultures for personalized medicine[J]. Cells, 2018, 7(12):225.
[51]	GJOREVSKI N, SACHS N, MANFRIN A, et al. Designer matrices for intestinal stem cell and organoid culture[J]. Nature, 2016, 539(7630):560-564.
[52]	KIM S, MIN S, CHOI Y S, et al. Tissue extracellular matrix hydrogels as alternatives to Matrigel for culturing gastrointestinal organoids[J]. Nat Commun, 2022, 13(1):1692.
[53]	MOUSSEAU Y, MOLLARD S, QIU H, et al. In vitro 3D angiogenesis assay in egg white matrix:comparison to Matrigel, compatibility to various species, and suitability for drug testing[J]. Lab Invest, 2014, 94(3):340-349.
[54]	KIM S, CHOI Y S, LEE J S, et al. Intestinal extracellular matrix hydrogels to generate intestinal organoids for translational applications[J]. J Ind Eng Chem, 2022, 107:155-164.
[55]	RAHMAN S, GHIBOUB M, DONKERS J M, et al. The progress of intestinal epithelial models from cell lines to gut-on-chip[J]. Int J Mol Sci, 2021, 22(24):13472.
[56]	ZHANG J, TAVAKOLI H, MA L, et al. Immunotherapy discovery on tumor organoid-on-a-chip platforms that recapitulate the tumor microenvironment[J]. Adv Drug Deliv Rev, 2022, 187:114365.
[57]	PETERSON L W, ARTIS D. Intestinal epithelial cells:regulators of barrier function and immune homeostasis[J]. Nat Rev Immunol, 2014, 14(3):141-153.
[58]	WILSON S S, TOCCHI A, HOLLY M K, et al. A small intestinal organoid model of non-invasive enteric pathogen-epithelial cell interactions[J]. Mucosal Immunol, 2015, 8(2):352-361.
[59]	YANG N, ZHANG Y H, FU Y G, et al. Transmissible gastroenteritis virus infection promotes the self-renewal of porcine intestinal stem cells via Wnt/β-catenin pathway[J]. J Virol, 2022, 96(18):e00962-22.
[60]	NASH T J, MORRIS K M, MABBOTT N A, et al. Inside-out chicken enteroids with leukocyte component as a model to study host-pathogen interactions[J]. Commun Biol, 2021, 4(1):377.

Animal Intestinal Organoids Culture

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