基于SNP标记的滩羊亲子鉴定研究

doi:10.11843/j.issn.0366-6964.2022.09.009

Abstract

Abstract: The purpose of this study was to screen some SNP markers that can be used for paternity identification of Tan sheep, which is of great significance for the conservation and reproduction of sheep. A total of 159 Tan sheep in the Yanchi area of Ningxia was selected as the research object. The 600K gene microarray was used for SNP determination. The genetic background of the sample was explored through principal component analysis. The family lineage was divided according to the phylogenetic tree. A paternity test was conducted. The results showed that most of the Tan sheep had similar genetic backgrounds, and the phylogenetic tree divided them into 5 major lineages; The 211 high-quality SNPs were screened, and the single parent cumulative exclusion probability (CPE) exceeded 99.99%, with high accuracy and reliability. The results of paternity identification show that at the 95% confidence level, the observed identification rate was 79%, and under the 80% confidence level, the observed identification rate reached 87%. In the results, most of the real parents were found in the suspected male parents, that is, the male parents with no pedigree records, indicating that the pedigree management should be strengthened in the field to avoid errors as much as possible.

Key words: Tan sheep, SNP markers, paternity identification

CLC Number:

S826.2

LI Ling, LI Yefang, LIANG Benmeng, SUN Yujiang, MA Yuehui, MA Qing, JIANG Lin, LIU Shuqin. Paternity Identification of Tan Sheep Based on SNP Markers[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2912-2919.

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Paternity Identification of Tan Sheep Based on SNP Markers

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