吸血行为对草原革蜱和森林革蜱中肠菌群结构的影响

doi:10.11843/j.issn.0366-6964.2020.01.015

Abstract

Abstract: The aim of this study is to investigate the characteristics and changes of bacterial flora in midguts of Dermacentor nuttalli and Dermacentor silvarum with the extension of feeding time. The half or fully engorged D. nuttalli and D. silvarum were obtained from sheep body surface in Hulun Buir of Inner Mongolia and Guyuan of Ningxia, respectively. The midgut contents were collected from ticks under sterile environment. Then the total DNA of bacteria were extracted and the V3-V4 areas of 16S rDNA were amplified. The PCR product of each sample was sequenced by IonS5^TMXL high-throughput sequencing platform. Each sample's bacterial flora characteristic and the differences among four groups (half engorged D.nuttalli, fully engorged D.nuttalli,half engorged D.silvarum and fully engorged D.silvarum) were analyzed. The results showed that the bacterial diversity of midguts from the half engorged D. silvarum was the highest, followed by that of midguts from half and fully engorged D. nuttalli, the bacterial diversity of midguts from the fully engorged D. silvarum was the lowest. Proteobacteria was the most predominant phyla in all samples. Anaplasma, Rickettsia, Stenotrophomonas and Coxiella were the major genera. The relative abundance of Anaplasma in midguts from fully engorged D. nuttalli and D. silvarum were higher than that in the midguts from the half engorged ticks. The relative abundance of Rickettsia and Coxiella in midguts from half engorged D. nuttalli and D. silvarum were higher than that in the midguts from the fully engorged ticks. Anaplasma marginale, Pseudomonas geniculate and Coxiellaceae bacterium RFE02 were the most predominant species in four samples. Distributions of A. marginale in the midguts of D. nuttalli and D. silvarum were consistent with that of Anaplasma. These findings suggested that the midgut bacterial flora of D. nuttalli and D. silvarum are susceptible to the blood-sucking behavior. The relative abundance of common bacteria genera and species vary greatly in different tick species and engorged statuses.

Key words: Dermacentor nuttalli, Dermacentor silvarum, midgut, microbial community structure, blood-sucking behavior

CLC Number:

S852.746

DUAN Deyong, ZHOU Hongming, CHENG Tianyin. Effects of Blood-sucking Behavior on the Microbial Community in the Midgut of Dermacentor nuttalli and Dermacentor silvarum[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(1): 128-136.

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Effects of Blood-sucking Behavior on the Microbial Community in the Midgut of Dermacentor nuttalli and Dermacentor silvarum

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