ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2017, Vol. 48 ›› Issue (9): 1777-1784.doi: 10.11843/j.issn.0366-6964.2017.09.025

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Study on Toxicity of Fumonisin B1 to Human Umbilical Vein Endothelial Cells

CAO Xi-feng, QIN Wei-sen, XIONG Yong-xuan, LI Nan, DENG Xian-bai*   

  1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
  • Received:2017-04-05 Online:2017-09-23 Published:2017-09-23

Abstract:

To investigate the toxicity and mechanism of action of fumonisin B1 (FB1) on human umbilical vein endothelial cells (HUVEC), HUVEC cells were treated with FB1 in different concentration. The cell vitality, cell cycle, cell oxidative stress, apoptosis, mitochondrial membrane potential change and transcription changes of apoptosis-related genes were detected by Cell Counting Kit-8, flow cytometry, fluorescence microscope, Hoechst33342 staining and real-time quantitative PCR. In result, the vitality of FB1 treated cells (FB1 dose exceed 5 μg·mL-1 and process time exceed 12 h) were significantly decrease (P<0.01 or P<0.05). And the fluorescence intensity of reactive oxygen active (ROS) were enhanced when being treated with 10, 20 μg·mL-1 of FB1. FB1 could prevent HUVEC from G0/G1 phase to S phase and reduce the mitochondrial membrane potential. The transcription of apoptosis gene Caspase-3 were increased, Bcl-2 were significantly declined (P<0.05). Caspase-9 and Bax genes transcriptions were significantly increased (P<0.01). The results showed that FB1 has cytotoxic effect on HUVEC cells, which could inhibit cell proliferation, arrest cell cycle at G0/G1 phase and induce apoptosis via mitochondrial pathway. Results in the present works provided the foundations for further research of toxicity and mechanism of FB1 on human cells and indications for some relevant diseases treatment.

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