马鹿鹿茸不同部位差异蛋白质组学分析

doi:10.11843/j.issn.0366-6964.2017.08.004

Abstract

Abstract:

This study aimed to provide theoretical basis for clarifying velvet antler biological characteristics by comparative proteomics. In this study, different parts of Cervus elaphus songaricus velvet antler (on 65 and 75 days old) were studied by two-dimensional gel electrophoresis, MALDI-TOF-MS mass spectrometry identification and bioinformatics analysis. 65 differentially expressed proteins were successfully identified, and these proteins were involved in the cellular process, developmental process, localization, metabolic process, response to stimulus, and the KEGG pathway of platelet activation, focal adhesion, metabolic pathways, PI3K-Akt signaling pathway. These proteins were associated with bone development (4 proteins), nerve development (3 proteins), vascular development (2 proteins), anti-oxidation and endoplasmic reticulum stress (4 proteins), immune (7 proteins), apoptosis (5 proteins). The results indicated that TTR, RBP4 and CRABP1 played important roles in regulating the growth and development of velvet antler in the process of antler's transition from 65 d to 75 d velvet antler. PRDX2, TXNDC5, ERP29, Pdia6 played important roles in endoplasmic reticulum stress and anti-oxidation. Endoplasmic reticulum stress, endoplasmic reticulum-related death pathway and mitochondrial apoptotic pathways could explain the rapid differentiation of velvet antler cells while a large amount of apoptosis, which could meet the rapid growth and development needs of velvet antler. In addition, GMFβ and CATHL1 may play important roles in nerve development and anti-inflammatory.

CLC Number:

S825.2

WANG Lei, ZHANG Ran-ran, LIU Hua-miao, LIU Hui-tao, ZHOU Yong-na, DONG Shi-wu, XING Xiu-mei. Comparative Proteomics Analysis on Different Parts of Cervus elaphus songaricus Velvet Antler[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(8): 1401-1415.

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Comparative Proteomics Analysis on Different Parts of Cervus elaphus songaricus Velvet Antler

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