ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (6): 1147-1153.doi: 10.11843/j.issn.0366-6964.2016.06.009

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Screening of Candidate Genes for Horn Trait of Yak

SHE Ping-chang1,2,WU Xiao-yun1,2,LIANG Chun-nian1,2,CHU Min1,2,DING Xue-zhi1,2,YAN Ping1,2*   

  1. (1.Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences,Lanzhou 730050,China;2. Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou 730050,China)
  • Received:2015-11-02 Online:2016-06-23 Published:2016-06-23

Abstract:

This study screened the candidate genes of horn trait which provides scientific basis for the horn developmental mechanism and the polled yak breeding.The horn bud and forehead skin tissue from horn and polled yaks with 90 d gestational age were collected,and the mRNA different expression of OLIG1,OLIG2,IFNAR1,IFNAR2,C1H21orf62,GCFC1,IFNGR2,SYNJ1,IL10RB,GART,RXFP2,FOXL2,TWIST1,TWIST2,ZEB2,E-cadherin,P-cadherin genes were analyzed using real-time quantitative PCR(qRT-PCR).The results showed that expressions of OLIG1,IFNAR1,C1H21orf62,SYNJ1,IL10RB,GART,RXFP2,TWIST2 and E-cadheringenes showed no significant differences in horn and skin between horned and polled yaks (P>0.05).There were significant difference at transcript levels of IFNGR2,FOXL2,TWIST1 and P-cadherin genes in horn buds between horned and polled yaks(P<0.05).IFNAR2 and GCFC1 had significant transcription difference in the expression of horn bud and skin between horned and polled yaks (P<0.05).OLIG2 and ZEB2 had highly significant transcription difference in horn bud expression between horned and polled yaks (P<0.01).We concluded that OLIG2 and FOXL2 might be the key candidate genes.ZEB2,TWIST1 and IFNGR2 might also involve in the formation of horn. P-cadherin might participate in the development of horn.

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