（College of Life Science and Technology，Southwest University for Nationalities/ Innovative Research Team on Animal Epidemic Prevention and Control of Education Department of Sichuan，Chengdu 610041，China）
ZHOU Fang，YUE Hua，ZHANG Bin，LI Fan，CHEN Xi，TANG Cheng. Establishment and Application of an RT-PCR Assay for Yak Rotavirus Based on the Sequence Analysis of Yak Rotavirus VP6 gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1465-1473.
［1］BADARACCO A，GARAICOECHEA L，RODRÍGUEZ D，et al．Bovine rotavirus strains circulating in beef and dairy herds in Argentina from 2004 to 2010［J］．Vet Microbiol，2012，158(3-4)：394-399．
［2］MINAMI-FUKUDA F，NAGAI M，TAKAI H，et al.Detection of bovine group A rotavirus using rapid antigen detection kits，RT-PCR and next-generation DNA sequencing［J］.JVet Med Sci，2013，75(12)：1651-1655.
［3］ALFIERI A F，ALFIERI A A，BARREIROS M A，et al.G and P genotypes of group A rotavirus strains circulating in calves in Brazil 1996-1999［J］.Vet Microbiol，2004，99(3-4)：167-173.
［4］GONZALEZ D D，MOZGOVOJ M V，BELLIDO D，et al.Evaluation of a bovine rotavirus VP6 vaccine efficacy in the calf model of infection and disease［J］.Vet ImmunolImmunopathol，2010，137(1-2)：155-160.
［5］WISE A G，SMEDLEY R C，KIUPEL M，et al.Detection of group C rotavirus in juvenile ferrets (Mustela putorius furo) with diarrhea by reverse transcription polymerase chain reaction：sequencing and analysis of the complete coding region of the VP6 gene［J］.Vet Pathol，2009，46(5)：985-991.
［6］STIPP D T，ALFIERI A F，LORENZETTI E，et al.VP6 gene diversity in 11 Brazilian strains of porcine group C rotavirus［J］.Virus Genes，2015，50(1)：142-146.
［7］范晴，谢芝勋，刘加波，等.牛轮状病毒TaqMan实时荧光RT-PCR快速检测方法的建立［J］.中国畜牧兽医，2011，38(4)：105-108.
FAN Q，XIE Z X，LIU J B，et al.Detection of bovine rotavirus by TaqMan based real-time reverse transcription polymerase chain reaction assay［J］.China Animal Husbandry &Veterinary Medicine，2011，38(4)：105-108.(in Chinese)
［8］王洁清.牛轮状病毒RT-PCR检测和基于VP6重组抗原检测血清抗体ELISA方法初步建立［D］.哈尔滨：东北农业大学，2009.
WANG J Q.Detection of bovine rotavirus by RT-PCR and establishment of indirect ELISA to detect bovine rotavirus antibody with VP6 protein［D］.Harbin：Northeast Agricultural University，2009.(in Chinese)
［9］BASERA S S，SINGH R，VAID N，et al.Detection of rotavirus infection in bovine calves by RNA-PAGE and RT-PCR［J］. Indian J Virol，2010，21(2)：144-147.
［10］NYAGA M M，ESONA M D，JERE K C，et al.Genetic diversity of rotavirus genome segment 6(encoding VP6) in Pretoria，South Africa［J］. Springerplus，2014，3：179.
［11］KERIN T K，KANE E M，GLASS R I，et al.Characterization of VP6 genes from rotavirus strains collected in the United States from 1996-2002［J］. Virus Genes，2007，35(3)：489-495.
［12］CHEN X，ZHANG B，YUE H，et al.A novel astrovirus species in the gut of yaks with diarrhea in the Qinghai Tibetan plateau，2013［J］.J Gen Virol，2015.doi：10.1099/gv.0.000303.
［13］ITURRIZA GÓMARA M，WONG C，BLOME S，et al.Molecular characterization of VP6 genes of human rotavirus isolates：correlation of genogroups with subgroups and evidence of independent segregation［J］.J Virol，2002，76(13)：6596-6601.
［14］GAUTAM R，ESONA M D，MIJATOVIC-RUSTEMPASIC S，et al.Real-time RT-PCR assays to differentiate wild-type group A rotavirus strains from Rotarix and Rota Teq vaccine strains in stool samples［J］.Hum Vaccin Immunother，2014，10(3)：767-777.
［15］LIN Y P，KAO C L，CHANG S Y，et al.Determination of human rotavirus VP6 genogroups Ⅰ and Ⅱ by reverse transcription-PCR［J］.J Clin Microbiol，2008，46(10)：3330-3337.
［16］DENNEHY P H.Rotavirus vaccines：an overview［J］.Clin Microbiol Rev，2008，21(1)：198-208.
［17］LAPPALAINEN S，PASTOR A R，MALM M，et al.Protection against live rotavirus chanllenge in mice induced by parenteral and mucosal delivery of VP6 subunit rotavirus vaccine［J］. Arch Virol，2015，160(8)：2075-2078.
［18］MATTHIJNSSENS J，TARAPOREWALA Z F，YANG H，et al.Simian rotaviruses possess divergent gene constellations that originated from interspecies transmission and reassortment［J］.J Virol，2010，84(4)：2013-2026.
［19］MATTHIJNSSENS J，CIARLET M，HEIMAN E，et al.Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and procine rotavirus strains and human DS-1-like and bovine rotavirus strains［J］.J Virol，2008，82(7)：3204-3219.
［20］DURMAZ R，KALAYCIOGLU A T，ACAR S，et al.Prevalence of rotavirus genotypes in children younger than 5 years of age before the introduction of a universal rotavirus vaccination program：report of rotavirus surveillance in Turkey［J］.PLoS One，2014，9(12)：e113674.
［21］DOAN Y H，NAKAGOMI T，ABOUDY Y，et al.Identification by full-genome analysis of a bovine rotavirus transmitted directly to and causing diarrhea in a human child［J］.J ClinMicrobiol，2013，51(1)：182-189.
