高原地区小尾寒羊附睾细胞外基质相关蛋白分布特征研究

doi:doi: 10.11843/j.issn.0366-6964.2016.08.006

Abstract

Abstract:

To explore the distribution of extracellular matrix proteins in epididymis of Small-tail Han sheep in plateau.The histochemistry of Masson’s，Gomori’s，PAS and AB-PAS（pH=2.5）staining were used to study the microstructure of epididymis in 5 pairs of Small-tail Han sheep in plateau，and the distribution of the laminin (LN)，type Ⅳ collagen (Col Ⅳ) and heparan sulfate glycoprotein (HSPG) were identified by SP immunohistochemical method.The observations made with the light microscope showed that the epithelium of epididymal ducts were columnar ciliated and the distribution of the collagen and reticular fibers in the cauda were more abundant than the caput and the corpus，although the PAS positive reaction in the caput and the cauda were stronger than the corpus，but the AB-PAS positive reaction without significant differences in the caput，the corpus and the cauda.Immunohistochemical analysis appeared that Col Ⅳ was strongly presented in the caput and the corpus，the relative expression of HSPG was lower than Col Ⅳ，and LN was expressed weakly，the distribution of LN and HSPG without statistical differences in epididymis (P＞0.05)，but in the cauda，it was showed that HSPG was strongly presented，the Col Ⅳ was lower than LN and HSPG，and the distribution of LN and HSPG without statistical differences in epididymis (P＞0.05).Taken together，the collagen and reticular fibers of the epididymis cauda were more abundant than the caput and corpus in Small-tail Han sheep from plateau，and the positive of the PAS reaction was companied with the changes of the secretion function of epithelium.The secretion of Col Ⅳ，HSPG and LN in the basal cells of caput epithelium were increased than the other parts，and the Col Ⅳ，HSPG and LN might work together closely for the regulation of the vessel.

CLC Number:

S852.16+2

LI Cong，YUAN Li-gang，ZHANG Yong. The Distribution of Extracellular Matrix Proteins in Small-tail Han Sheep Epididymis in Plateau[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1565-1573.

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The Distribution of Extracellular Matrix Proteins in Small-tail Han Sheep Epididymis in Plateau

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