副猪嗜血杆菌potD基因缺失株的构建及其部分生物学特性

doi:10.11843/j.issn.0366-6964.2016.11.015

Abstract

Abstract:

As a transporter, protein encoded by potD is involved in the binding and transportation of polyamine, which is necessary for the growth of cells. In some pathogens, the protein is identified as a virulence-associated factor, but of which the function is still unclear in Haemophilus parasuis. A potD mutant strain SC1401ΔpotD::kan was constructed by the natural transformation method. The growth curve, the ability of autoagglutination and biofilm formation, serum resistance ability and the virulence to mice of the parental strain SC1401 and potD mutant strain were measured. No significant difference was observed between the parental and mutant strains in the growth curve and the ability of autoagglutination and biofilm formation. However the potD mutant strain showed an obvious decrease in serum resistance ability and the virulence to mice. The findings above suggested that potD gene may be associated with virulence in H. parasuis.

CLC Number:

S852.613

JIN Jin, ZHANG Lu-hua, WEN Xin-tian,LI Ying, DAI Ke, ZHOU Peng, ZHAO Yu-jia, CAO San-jie, HUANG Xiao-bo, WU Rui, ZHAO Qin, WEN Yi-ping. Construction and Characterization of a Haemophilus parasuis potD Mutant Strain[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(11): 2274-2279.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2016.11.015

https://www.xmsyxb.com/EN/Y2016/V47/I11/2274

References

［1］XU Z, YUE M, ZHOU R, et al. Genomic characterization of Haemophilus parasuis SH0165，a highly virulent strain of serovar 5 prevalent in China［J］. PLoS One, 2011, 6(5): e19631.
［2］TURNI C, BLACKALL J. Comparison of the indirect haemagglutination and gel diffusion test for serotyping Haemophilus parasuis［J］. Vet Microbiol, 2005, 106(1-2): 145-151.
［3］ZHANG L H, WEN Y P, LI Y, et al. Comparative proteomic analysis of the membrane proteins of two Haemophilus parasuis strains to identify proteins that may help in habitat adaptation and pathogenesis［J］. Proteome Sci, 2014, 12: 38.
［4］WARE D, JIANG Y, LIN W, et al. Involvement of potD in Streptococcus pneumoniae polyamine transport and pathogenesis［J］. Infect Immun, 2006, 74(1): 352-361.
［5］ZHANG X, ZHANG Y, LIU J, et al. PotD protein stimulates biofilm formation by Escherichia coli［J］. Biotechnol Lett, 2013, 35(7): 1099-1106.
［6］NASRALLAH G K, ABDELHADY H, TOMPKINS N P, et al. Deletion of potD, encoding a putative spermidine-binding protein, results in a complex phenotype in Legionella pneumophila ［J］. Int J Med Microbiol, 2014, 304(5-6): 703-716.
［7］ZHANG L H, LI Y, DAI K, et al. Establishment of a successive markerless mutation system in Haemophilus parasuis through natural transformation［J］. PLoS One, 2015, 10(5): E0127393.
［8］ZOU Y, FENG S, XU C, et al. The role of galU and galE of Haemophilus parasuis SC096 in serum resistance and biofilm formation［J］. Vet Microbiol, 2013, 162(1): 278-284.
［9］ZHANG B, FENG S, XU C, et al. Serum resistance in Haemophilus parasuis SC096 strain requires outer membrane protein P2 expression［J］. FEMS Microbiol Lett, 2012, 326(2): 109-115.
［10］JOHNSBORG O, ELDHOLM V, HÅVARSTEIN L S. Natural genetic transformation: prevalence, mechanisms and function［J］. Res Microbiol, 2007, 158(10): 767-778.
［11］MERCIER A, BERTOLLA F, PASSELÈGUE-ROBE E, et al. Influence of DNA conformation and role of comA and recA on natural transformation in Ralstonia solanacearum ［J］. Can J Microbiol, 2009, 55(6): 762-770.
［12］REDFIELD R J, FINDLAY W A, BOSSÉ J, et al. Evolution of competence and DNA uptake specificity in the Pasteurellaceae［J］. BMC Evol Biol, 2006, 6: 82.
［13］孙东昌，张衍梅，施跃峰. 细菌自然转化的分子机制研究进展［J］. 微生物学报, 2012, 52(1): 6-11.
SUN D C, ZHANG Y M, SHI Y F. Advances in the molecular mechanism of natural bacterial transformation［J］. Acta Microbiologica Sinica, 2012, 52(1): 6-11. (in Chinese)
［14］BOSSÉ J T, SINHA S, SCHIPPERS T, et al. Natural competence in strains of Actinobacillus pleuropneumoniae ［J］. FEMS Microbiol Lett, 2009, 298(1): 124-130.
［15］BIGAS A, GARRIDO M E, DE ROZAS A M , et al. Development of a genetic manipulation system for Haemophilus parasuis ［J］. Vet Microbiol, 2005, 105(3-4): 223-228.
［16］HE L Q, WEN X T, YAN X F, et al. Effect of cheY deletion on growth and colonization in a Haemophilus parasuis serovar 13 clinical strain EP3［J］. Gene, 2015, 577(1): 96-100.
［17］曾泽，何欢，岳华，等. 副猪嗜血杆菌lgtF基因缺失株的构建及其部分生物学特性［J］. 畜牧兽医学报, 2015, 46(11): 2056-2062.
ZENG Z, HE H, YUE H, et al. Construction and characterization of a Haemophilus parasuis SC096 ΔlgtF mutant strain.［J］. Acta Veterinaria et Zootechnica Sinica, 2015, 46(11): 2056-2062. (in Chinese)

[1]	XIAO Jing, XIAO Kunxue, WANG Qiaochu, CHEN Huanchun, CAI Xuwang, XU Xiaojuan. Development and Optimization of Double-Gene Knockout Method for Glaesserella parasuis based on the Flp-FRT System [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 219-230.
[2]	TIAN Yang, LIU Yunbao, MA Hui, PAN Qicong, XIAO Jing, CHEN Huanchun, CAI Xuwang, XU Xiaojuan. Development and Preliminary Application of Apd-ELISA Kit for Antibody Detection of Haemophilus parasuis [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(9): 2227-2237.
[3]	TANG Mengjun, ZHOU Qian, ZHANG Xiaoyan, ZHANG Jing, TANG Xiujun, LU Junxian, ZHOU Sheng, PU Junhua, GAO Yushi. Antimicrobial Resistance and Virulence Genes of Campylobacter spp. Isolated from Chicken and Swine Farms in Jiangsu Province [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(9): 2284-2292.
[4]	SHEN Yi-juan, ZHOU Ni-ni, ZHANG Jian-song, WANG Mei-fen, LI Yu-feng, JIANG Ping. TGF-β1 Signaling Stimulates Invasion of Haemophilus parasuis into 3D4/21 Cells [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1720-1726.
[5]	CHENG Lei, CHEN Hong-bo. The Role of Macrophage-γδ T Cell Immune Axis in the Pathogenesis of Glässer's Disease [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(5): 879-886.
[6]	YANG Kai-jie, FENG Sai-xiang, ZHOU Qi, ZHANG Jian-min, LIAO Ming, FAN Hui-ying. Effect of qseC Gene Mutation of Haemophilus parasuis on Biofilm Formation [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(8): 1499-1504.
[7]	HE Huan，CHEN Xin-nuo，ZENG Ze，REN Yu-peng，TANG Cheng，ZHANG Bin，YUE Hua. The Initial Research of OmpP2 in Haemophilus parasuis Induces Pro-inflammatory Cytokine mRNA Transcription and Inflammatory Mechanism in Porcine Alveolar Macrophages [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1428-1434.
[8]	ZENG Ze，HE Huan，REN Yu-peng，YUE Hua，ZHANG Bin. The Effect of rfaE Gene in Haemophilus parasuis Lipooligosaccharide Induced Inflammation via the MAPKs/ NF-κB Signaling Pathways in Porcine Alveolar Macrophages [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 978-984.
[9]	ZENG Ze，HE Huan，YUE Hua，TANG Cheng，ZHANG Bin. Construction and Characterization of a Haemophilus parasuis SC096 ΔlgtF Mutant Strain [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(11): 2056-2062.
[10]	ZENG Ze，YUE Hua，FENG Xiao-hui，HE Huan，ZHANG Bin. The Effect of rfaE Gene in Haemophilus parasuis LOS Induces Pro-inflammatory Cytokine mRNA Transcription in Porcine Alveolar Macrophages [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1232-1237.
[11]	BAI Ai-quan，GUO Jian-chao，QIN Zong-hua，PU Wen-jun，MA Chun-quan，LI Guo-qing，ZHANG Hao-ji. Development of a TaqMan Quantitative Polymerase Chain Reaction Assay for Detecting Lawsonia intracellularis [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(10): 1733-1738.
[12]	KUANG Xue-qian, YUE Hua, CHEN Xiao-fei, TANG Cheng. The Research of Campylobacter jejuni cmp Gene Structure and ST Type in Livestock and Poultry [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(4): 657-664.

Construction and Characterization of a Haemophilus parasuis potD Mutant Strain

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 12

Recommended Articles

Metrics

Comments