Dmrt7在绵羊不同发育期睾丸组织中的表达与定位

doi:10.11843/j.issn.0366-6964.2016.12.008

Abstract

Abstract:

In order to understand the expression and cellular localization of double sex and mab-3 related transcription factor 7 (Dmrt7) in Small-Tail Han sheep testis tissues, fifteen sheep testis tissues were collected at 0, 2, 6, 12 and 24-month-old stages (3 replicates each), and the expression and cellular localization of Dmrt7 on testis tissues were detected by qRT-PCR, Western blot and immunohistochemistry. The results showed that Dmrt7 mRNA and protein were detected in sheep testes at different months of age, and the change pattern was similar. But, there were differences concerning the expression level of Dmrt7 for the different month groups. Dmrt7 mRNA and protein were lowly expressed in 0, 2 and 6 -month-old sheep testes, but it were highly expressed in 12, 24-month-old sheep testes. Meanwhile, the expression of Dmrt7 mRNA and protein in 12 and 24-month-old sheep testis tissues were extremely significantly higher than those in 0, 2 and 6-month-old sheep testes tissues（P<0.01）. The immunohistochemically staining revealed that immunoreactivity of Dmrt7 protein was localized to minority of spermatogonia in 0, 2 and 6-month-old sheep testis tissues, but it was mainly localized to secondary spermatocytes and partial primary spermatocytes and spermatids in 12 and 24-month-old sheep testis tissues. The results demonstrated that Dmrt7 played an essential regulation role to the division processes of various spermatogenic cells in testicular seminiferous tubule, especially meiotic division from primary spermatocytes to spermatids.

CLC Number:

LI Tao-tao，GUO Ya-min，MA You-ji，ZHAO Xing-xu，LU Zeng-kui，LUO Rui-rui， GAO Jian-feng. Expression and Cellular Localization of Dmrt7 in Testis of Sheep at Different Developmental Stages[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(12): 2390-2397.

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References

［1］王锋.动物繁殖学［M］.北京:中国农业大学出版社，2012：316-320.
WANG F. Animal reproduction［M］. Beijing: China Agricultural University Press, 2012:316-320. (in Chinese)
［2］SHARPE R M. Environmental/lifestyle effects on spermatogenesis［J］. Phil Trans R Soc B, 2010, 365(1546):1697-1712.
［3］葛少钦，康现江，刘桂荣，等.精子发生过程中的相关基因［J］.遗传，2008, 30(1):3-12.
GE S Q, KANG X J, LIU G R, et al. Genes involved in spermatogenesis［J］. Hereditas, 2008, 30(1):3-12. (in Chinese)
［4］O’BRIEN K L O, VARGHESE A C, AGARWAL A. The genetic causes of male factor infertility: A review［J］. Fertil Steril, 2010, 93(1):1-12.
［5］刘艳.LRGUK在小鼠精子发生过程的功能研究［D］.郑州:郑州大学，2015.
LIU Y. The function of LRGUK on spermatogenesis of mice［D］. Zhengzhou: Zhengzhou University, 2015. (in Chinese)
［6］SCHLECHT U, DEMOUGIN P, KOCH R, et al. Expression profiling of mammalian male meiosis and gametogenesis identifies novel candidate genes for roles in the regulation of fertility［J］. Mol Biol Cell, 2004, 15(3):1031-1043.
［7］丁小举，王朝亮，张卫星，等.基因敲除技术在精子发生相关基因功能研究中的应用［J］.中华男科学杂志，2014, 20(9):850-853.
DING X J, WANG C L, ZHANG W X, et al. Application of gene knockout technology in spermatogenesis-associated gene study［J］. National Journal of Andrology, 2014, 20(9):850-853. (in Chinese)
［8］NARENDRA U, ZHU L, LI B, et al. Sex-specific gene regulation. The Doublesex DM motif is a bipartite DNA-binding domain［J］. J Biol Chem, 2002, 277(45):43463-43473.
［9］KAWAMATA M, NISHIMORI K. Mice deficient in Dmrt7 show infertility with spermatogenic arrest at pachytene stage［J］. Febs Lett, 2006, 580(27):6442-6446.
［10］KIM S, KETTLEWELL J R, ANDERSON R C, et al. Sexually dimorphic expression of multiple doublesex-related genes in the embryonic mouse gonad［J］. Gene Expr Patterns, 2003, 3(1):77-82.
［11］RAYMOND C S, MURPHY M W, O'SULLIVAN M G, et al. Dmrt1, a gene related to worm and fly sexual regulators, is required for mammalian testis differentiation［J］. Gene Dev, 2000, 14(20):2587-2595.
［12］HOU Y, ZHOU X, LIU J, et al. Nuclear factor-Y (NF-Y) regulates transcription of mouse Dmrt7 gene by binding to tandem CCAAT boxes in its proximal promoter［J］. Int J Biol Sci, 2010, 6(7):655-664.
［13］KIM S, NAMEKAWA S H, NISWANDER L M, et al. A mammal-specific Doublesex homolog associates with male sex chromatin and is required for male meiosis［J］. PLoS Genet, 2007, 3(4): e62.
［14］HERRERA J M, MCNEIL K, WELLS E, et al. Male-specific function of Dmrt7 by sexually dimorphic translation in mouse testis［J］. Sex Dev, 2007, 1(5):297-304.
［15］HANDEL M A. The XY body: a specialized meiotic chromatin domain［J］. Exp Cell Res, 2004, 296(1):57-63.
［16］Hoyer-Fender S. Molecular aspects of XY body formation［J］. Cytogenet Genome Res, 2003, 103(3-4):245-255.
［17］金帅. Dmrt7低表达水平与犏牛精子发生阻滞相关性研究［D］.兰州：兰州大学，2013.
JIN S. The low expression of Dmrt7 is associated with spermatogenic arrest in cattle-yak［D］.Lanzhou: Lanzhou University, 2013. (in Chinese)
［18］淮亚红，许尚忠，姬爱国，等.牛Dmrt7基因cDNA的克隆和生物信息学分析［J］.西北农林科技大学学报(自然科学版)，2008, 36(3):13-17.
HUAI Y H, XU S Z, JI A G, et al. The cDNA cloning and biological information analysis of cattle Dmrt7［J］. Journal of Northwest A&F University (Natural Science Edition), 2008, 36(3):13-17. (in Chinese)
［19］葛玮.Dmrt7基因重组腺病毒及腺相关病毒载体的构建及鉴定［D］.杨凌：西北农林科技大学，2010.
GE W. Construction and identification of recombinant adenovirus vectors and adeno-associated virus vectors of Dmrt7［D］. Yangling: Northwest Agriculture and Forestry University, 2010. (in Chinese)
［20］马红，王文涛，付博，等.精液品质及其体内和体外受精能力的相关性研究［J］.畜牧与兽医，2012, 44(3):27-30.
MA H, WANG W T, FU B, et al. Relationships among procine semen quality and fertility in vitro and in vivo［J］. Animal Husbandry & Veterinary Medicine, 2012, 44(3):27-30. (in Chinese)
［21］HANSEN J C, DEGUCHI Y. Selenium and fertility in animals and man--a review［J］. Acta Vet Scand, 1996, 37(1):19-30.
［22］任有蛇，秦小伟，郭丽娜，等. 日粮纳米锌水平对公羊睾丸和附睾Cu-ZnSOD表达的影响［J］. 畜牧兽医学报，2014，45(10):1622-1630.
REN Y S, QIN X W, GUO L N, et al. Effect of the supplementation of different nano-zinc levels on expression of copper zinc superoxide in testis and epididymis of ram lambs［J］. Acta Veterinaria et Zootechnica Sinica, 2014, 45(10):1622-1630.(in Chinese)
［23］YUE D, YAN L, LUO H, et al. Effect of vitamin E supplementation on semen quality and the testicular cell membranal and mitochondrial antioxidant abilities in Aohan fine-wool sheep［J］. Anim Reprod Sci, 2010, 118(2-4):217-222.
［24］LIU S, MASTERS D, FERGUSON M, et al. Vitamin E status and reproduction in sheep: potential implications for Australian sheep production［J］. Anim Prod Sci, 2014, 54(6):694-714.
［25］袁莉刚，曲亚玲，鲁玉荣，等.不同月龄高原型藏绵羊睾丸组织中蛋白基因产物9.5和神经肽Y的分布比较［J］.畜牧兽医学报，2016，47(1):71-78.
YUAN L G, QU Y L, LU Y R, et al. Distribution of protein gene product 9.5 and neuropeptide Y in Tibetan Plateau sheep testis at different ages［J］. Acta Veterinaria et Zootechnica Sinica, 2016, 47(1):71-78. (in Chinese)
［26］马友记，卢曾奎，张全伟，等. CDK1和CyclinB1在不同月龄绵羊睾丸中的表达与定位［J］. 农业生物技术学报，2016，24(3):342-348.
MA Y J, LU Z K, ZHANG Q W, et al. Expression and cellular localization of CDK1 and CyclinB1 in the sheep(Ovis aires) testes of different months［J］. Journal of Agricultural Biotechnology, 2016, 24(3):342-348. (in Chinese)
［27］金帅，郭宪，包鹏甲，等.牦牛和犏牛Dmrt7基因序列分析及其在睾丸组织中的表达水平［J］.中国农业科学，2013, 46(5):1036-1043.
JIN S, GUO X, BAO P J, et al. Sequence analysis and study on the expression level of Dmrt7 gene in yak and cattle-yak testis［J］. Scientia Agricultura Sinica, 2013, 46(5):1036-1043. (in Chinese)
［28］DATE S, NOZAWA O, INOUE H, et al. Impairment of pachytene spermatogenesis in Dmrt7 deficient mice, possibly causing meiotic arrest［J］. Biosci Biotech Bioch, 2012, 76(9):1621-1626.

Expression and Cellular Localization of Dmrt7 in Testis of Sheep at Different Developmental Stages

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