EGF对牦牛冻精运动性能、细胞凋亡和IVF胚胎发育潜力的影响

doi:10.11843/j.issn.0366-6964.2017.05.009

Abstract

Abstract:

The study was to explore the effects of epidermal growth factor (EGF) on the apoptosis and motility of yak frozen sperm, the development competence of embryos after in vitro fertilization (IVF). Frozen-thawed spermatozoa of yak was incubated at 37.5 ℃ for 1 h in Tyrode's bicarbonate-buffered medium with EGF at different concentrations (0, 50, 75, 100, 150 and 200 ng·mL^-1). Sperm motility was measured by a computer-assisted sperm analyzer system; the expression of Bax and Bcl-2 in sperm were examined by qRT-PCR and Western blot; the development competence of embryo was also evaluated by using cleavage and blastocyst ratio after IVF. Our results showed that: 1) EGF dramatically enhanced spermatozoa motility and fertilized egg cleavage ratio (P<0.05), which were dose-dependence with EGF; 2) The Bcl-2 level was enhanced significantly with EGF at 75-150 ng·mL^-1 (P<0.05), while the Bax was reduced (P<0.05). In conclusion, EGF inhibited the apoptosis and increased the spermatozoa motility of yak frozen sperm during in vitro capacitation, but also enhanced the development competence of embryo after IVF, of which the optimal concentration was 75-150 ng·mL^-1. The present study was contributed to improve the yak embryos production in vitro referring to the growth factors.

CLC Number:

S823.8⁺5.2

PAN Yang-yang, WANG Meng, YU Si-jiu, CUI Yan, HE Jun-feng. The Effects of EGF on Yak Frozen Sperm Motility, Apoptosis and the Development Competence of Embryos after IVF[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 854-862.

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The Effects of EGF on Yak Frozen Sperm Motility, Apoptosis and the Development Competence of Embryos after IVF

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