ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (2): 367-373.doi: 10.11843/j.issn.0366-6964.2016.02.021

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Histopathological Observation in BALB/c Mice Experimentally Infected with Shigella boydii from Yak

RAN Dan-dan,ZHOU Fang,HUANG Zhi-hong,TANG Cheng*,LIU Ya-gang   

  1. (College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,China)
  • Received:2015-05-05 Online:2016-02-23 Published:2016-02-23

Abstract:

The objective of this study was to research the fast pathogenic damage of Shigella boydii from yak in BALB/c mice organs except intestine.The 4-week-old mouse were given 0.5 mL of Shigella boydii isolate(2×109 CFU•mL-1)by gavage.Tissue samples of cerebrum,heart,liver,spleen,lung,kidney,intestine and pancreas were taken from the infected mouse after being infected for 6,18,30,42,54 and 66 h,and ever since for every 12 hours to make tissue biopsies,which were then stained with haematoxylin and eosin (H.E) and immunohistochemistry to observe the changes of histopathology and the bacterial antigen localization in experimentally infected ducklings.Mouse serum were collected for biochemical indicators detection including lactate dehydrogenase,urea,creatinine,α amylase,triglyceride,total protein,albumin,phosphokinase,CO2 binding force,P,blood glucose and white blood cell count.The HE staining results showed a series of pathologic changes,not only the pathological damage in intestine epithelial cell as reports in other references,but also coagulation necrosis under the epicardium,cellular infiltration and granuloma in hepatic cells,inflammatory cells infiltrating in alveolar wall,pancreas epithelial cell swelling and cytoplasm dissolving and karyopyknosis or fragmentation.Immunochemistry staining results showed that positive bacterial antigen signals were found in cytoplasm of cardiac muscle cell,hepatocyte,alveolar wall,renal tubular epithelium cell,islet cell and intestinal epithelial cell.Pathological damages,heart and hepatic necrosis of Shigella boydii from yak experimentally infecting mice was first reported in this study.Eleven kinds of blood biochemical indexes of experimental mice changed apparently in each infected time point except triglyceride.This study verified that the severity of the pathology was positively correlated with the Shigella boydii antigen distribution.It was concluded that the isolate was the pantropic bacteria injuring most tissues and cells in mouse,and as well as intestinal,heart,liver,lung,pancreas and kidney were the principal target organs.

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