不同因素对疯草内生真菌合成苦马豆素的影响

doi:10.11843/j.issn.0366-6964.2015.01.021

Abstract

Abstract:

The research was conducted to screen the influence factors on swainsonine production in fungal endophyte，Undifilum oxytropis，from locoweed.U.oxytropis were inoculated in liquid medium of different pH or containing different concentration of PEG，L-pipecolic acid，L-lysine and ɑ-ketoglutaric acid，respectively.To estimate the influence of different factors on swainsonine production in U.oxytropis，the swainsonine of cultured fungal mycelia and zymotic fluid were extracted and detected after shaking culture.The results showed that low pH (pH4.5) inhibit significantly (P<0.05) the synthesis of swainsonine in fungi.With the increase of PEG in culture medium，the production of swainsonine in fungi was also inhibited significantly (P<0.01).When the fungi were inoculated respectively in liquid medium that contained equivalent concentration (10^-3 mol•L^-1) of L-pipecolic acid，L-lysine or ɑ-ketoglutaric acid，the production of swainsonine was inhibited significantly (P<0.05) in the group added L-pipecolic acid.However，the yield of swainsonine were increased significantly(P<0.05) in the group added L-lysine.When the initial concentration of L-pipecolic acid was 10^-3 and 10^-2 mol•L^-1，the production of swainsonine in U.oxytropis was inhibited significantly (P<0.05).However，when the initial concentration of L-pipecolic acid was 10^-4 mol•L^-1，the yield of swainsonine in U.oxytropis was increased significantly (P<0.01).When the initial concentration of L-lysine was 10^-1，10^-2 and 10^-4 mol•L^-1，the production of swainsonine in U.oxytropis was inhibited significantly (P<0.05).When the initial concentration of ɑ-ketoglutaric acid was 10^-1，10^-2 and 10^-3 mol•L^-1，the production of swainsonine in U.oxytropis was inhibited significantly(P<0.05).Low pH or PEG added to medium could significantly inhibit the production of swainsonine in U.oxytropis.It was significant that the influence of L-pipecolic acid，L-lysine and ɑ-ketoglutaric acid on the swainsonine producing of fungal endophyte.However，the extent of influence on fungal swainsonine producing was highly correlated with the concentration of L-pipecolic acid，L-lysine and ɑ-ketoglutaric in liquid media.

CLC Number:

S852.66

ZHANG Lei-lei，YU Yong-tao，HE Sheng-hu，ZHAO Qing-mei，GE Song. Influence of Different Factors on Swainsonine Production in Fungal Endophyte from Locoweed[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(1): 163-173.

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Influence of Different Factors on Swainsonine Production in Fungal Endophyte from Locoweed

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