Transcription and Positioning of Avian β-defensin 6 and Chicken TLR15 within the Intestinal Tract of Salmonella pullorum Infected Chicks
WANG Yan 1，ZHOU Xiu-hong 1，Qi Ke-zong1*，TU Jian1，LIU Hong-mei1，PAN Xiao-cheng 2
(1.College of Animal Science and Technology，Anhui Agricultural University，Hefei 230036，China；2.Anhui Academy of Agricultural Sciences，Hefei 230031，China)
WANG Yan,ZHOU Xiu-hong,Qi Ke-zong，TU Jian，LIU Hong-mei，PAN Xiao-cheng . Transcription and Positioning of Avian β-defensin 6 and Chicken TLR15 within the Intestinal Tract of Salmonella pullorum Infected Chicks[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(10): 1711-1717.
［1］CHAPPELL L，KAISER P，BARROW P，et al.The immunobiology of avian systemic salmonellosis ［J］.Vet Immunol Immunopathol，2009，128(1-3):53-59.
［2］PENG K S，RUAN L S，TU J，et al.Tissue distribution，expression，and antimicrobial activity of Anas platyrhynchos avian β-defensin 6［J］.Poult Sci，2013，92(1):97-104.
［3］HIGGS R，LYNN D J ，GAINES S.The synthetic form of a novel chicken β-defensin identified in silico is predominantly active against intestinal pathogens［J］.Immunogenetics，2005，57(1-2):90-98.
［4］CAO Y，MA Q，SHAN A，et al.Expression in Pichia Pastoris and biological activity of avian β-defensin 6 and its mutant peptide without cysteines［J］.Protein Pept Lett，2012，19(10):1064-1070.
［5］AKIRA S.Toll receptor families：structure and function［J］.Semin Immunol，2004，16(1):1-2.
［6］TEMPERLEY N D，BERLIN S，PATON I R，et al.Evolution of the chicken Toll-like receptor gene family：a story of gene gain and gene loss［J］.BMC Genomics，2008，9:62.
［7］HIGGS R，CORMICAN P，CAHALANE S，et al.Induction of a novel chicken Toll-like receptor following Salmonella enterica serovar Typhimurium infection ［J］.Infect Immun，2006，74(3):1692-1698.
［8］NERREN J R，HE H，GENOVESE K，et al.Expression of the avian-specific toll-like receptor15 in chicken heterophils is mediated by gram-negative and gran-positive bacteria，but not TLR agonists［J］.Vet Immunol Immunopathol，2010,136(1-2):151-156.
［9］ZIEGLER T R，EVANS M E，FERNÁNDEZ-ESTÍVARIZ C，et al.Trophic and cytoprotective nutrition for intestinal adaptation，mucosal repair，and barrier function［J］.Ann Rev Nutr，2003，23:229-261.
［10］江龙海，祁克宗．鸡β-防御素研究进展［J］．家禽科学，2007 (7)：36-37.
［11］VAN DIJK A，VELDHUIZEN E J，KALKHOVE S I，et al.The β-defensin gallinacin-6 is expressed in the chicken digestive tract and has antimicrobial activity against food-borne pathogens［J］.Antimicrob Agents Chemother，2007，51(3):912-922.
［12］RAMASAMY K T，VERMA P，REDDY M R.Differential gene expression of antimicrobial peptides β defensins in the gastrointestinal tract of Salmonella serovar Pullorum infected broiler chickens［J］.Vet Res Commun，2012，36 (1):57-62.
［13］ABASHT B，KAISER M G，VAN DER POEL J ，et al.Genetic lines differ in Toll-like receptor gene expression in spleens of chicks inoculated with Salmonella enterica serovar enteritidis ［J］.Poult Sci，2009，88(4)：744-749.
［14］ANASTASIADOU M，AVDI M，MICHAILIDIS G.Expression of avian β-defensins and Toll-like receptor genes in the rooster epididymis during growth and Salmonella infection［J］.Anim Reprod Sci，2013，140(3-4):224-231.
［15］MACKINNON K M，HE H,NERREN J R,et al.Expression profile of toll-like receptors within the gastrointestinal tract of 2-day-old Salmonella enteriditis-infected broiler chickens ［J］ .Vet Microbiol，2009，137(3-4)：313-319.
［16］周作勇，聂奎，宋振辉，等.雏鸡不同组织TLR1、TLR2、TLR4、TLR5和TLR15 mRNA转录水平相对定量研究［J］.畜牧兽医学报，2010，41（11）：1453-1459.
［17］MANICASSAMY S，PULENDRAN B.Modulation of adaptive immunity with Toll-like receptors［J］.Semin Immunol，2009，21(4):185-193.
［18］VORA P，YOUDIM A，THOMAS L S，et al.β-defensin-2 expression is regulated by TLR signaling in intestinal epithelial cells［J］.J Immunol，2004，173(9):5398-5405.
［19］YANG Y，JIANG Y，YIN Q，et al.Chicken intestine defensins activated murine peripheral blood mononuclear cells through the TLR4-NF-κB pathway［J］.Vet Immunol Immunopathol，2010，133(1):59-65.
