ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (9): 1417-1423.doi: 10.11843/j.issn.0366-6964.2014.09.006

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DNA Methylation and Imprinting Status of Meg8 in Somatic Cell Nuclear Transfer Bovines

ZHAO Shu-jun1,HU Jia-qi1,ZHANG Ming-yue1,LI Dong-jie2,DAI Yun-ping3,LI Ning3 ,LI Shi-jie1*   

  1. (1.College of Life Science,Agricultural University of Hebei,Baoding 071001,China;2.College of Bioscience & Bioengineering,Hebei University of Science and Technology,Shijiazhuang 050018,China;3.State Key Laboratory for Agrobiotechnology,China Agricultural University, Beijing 100193,China)
  • Received:2014-03-11 Online:2014-09-23 Published:2014-09-23

Abstract:

In order to determine the possible roles of Meg8 gene-internal CpG island in the regulation of the gene imprinting expression,the allele-specific DNA methylation status of 17 CpGs in intron3 of Meg8 in lung tissues was analyzed using bisulfite sequencing.The results demonstrated that the DNA methylation of both the parental strands were high (>80%),but T-strand showed significantly higher DNA methylation than that of C-strand(P<0.05) and the C-strand had only one kind of methylation model.Then the DNA methylation and imprinting status were analyzed in lung in somatic cell nuclear transfer(SCNT) bovine which died within postnatal 48 h.The results showed that the SCNT bovines exhibited significantly higher DNA methylation than that of naturally breed bovines(P<0.05).All of 17 CpGs showed complete methylation pattern in SCNT bovine.Imprinting analysis showed that Meg8 was mono-allelic expression in tissues of SCNT bovines.The DNA methylation of Meg8 intron3 CpGs island may not play a role in regulating of Meg8 imprinting expression.

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