中药治疗呼吸道疾病临床发病猪肺组织中PDE4、IL-6和TNF-α的变化

doi:10.11843/j.issn.0366-6964.2014.07.023

Abstract

Abstract:

The study was conducted to observe the variety of activity of phosphodiesterase 4 (PDE4)，expression of different subtype PDE4 and the content of IL-6，TNF-α in lung tissue of porcine respiratory disease complex，and evaluate the therapeutic effect and influence of self-made Chinese herbal prescription for accumulating information of respiratory inflammation pathological mechanism and its prevention.The pigs with respiratory disease were divided into disease group and treatment group，and normal ones as control group.The pigs of treatment group were drenched with Chinese herbal prescription (contain 1 g crude drug per mL)，which was composed of Reed Rhizome，Mongolian Milkvetch Root，White Peony Root and Liquorice Root，twice a day and 1.5 mL•kg^－1 of body weight for ten days.The changes of the number of white blood cell in peripheral blood were observed，and lung tissue of each group was collected.HPLC was used to detect the PDE4 activity.Real-time PCR was used to test the relative gene expression each subtype of PDE4，and ELISA kit was used to test the content of IL-6，TNF-α of lung tissue.The results were as follows：Compared with control group，the PDE4 activity，the content of IL-6 and the number of white blood cell in disease group increased significantly (P<0.05).The mRNA expression of PDE4A，4B，4C，4D and the content of TNF-α increased with indistinctive differences (P>0.05).Compared with the disease group，PDE4 activity decreased quite significantly (P<0.01)，the number of white blood cell and content of IL-6 in treatment group decreased significantly (P<0.05)，the content of TNF-α and mRNA expression of PDE4A，4C，4D decreased indistinctive differences (P>0.05)，while PDE4B reduced quite significantly (P<0.01).Abnormal inflammatory reaction exists in lung of porcine respiratory system disease，PDE4 activity，content of IL-6 and the number of white blood cell increase significantly.Self-made Chinese herbal prescription can improve the clinical symptoms of the disease，of which the function mechanism is related to the reduction of the number of white blood cell，inhibition of PDE4 activity and PDE4B expression，and reduction content of IL-6 in lung tissue.

CLC Number:

S853.31

ZHANG Mei-hua，ZHANG Qian，MENG Le，LI Jia，ZHOU Shuang-hai,JIANG Dai-xun，CHEN Wu. Variety of Phosphodiesterase4 and IL-6，TNF-α in Lung of Clinical Porcine Respiratory Disease under the Treatment of Chinese Herbal Medicine[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(7): 1189-1195.

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Variety of Phosphodiesterase4 and IL-6，TNF-α in Lung of Clinical Porcine Respiratory Disease under the Treatment of Chinese Herbal Medicine

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