围产期山羊血钙稳恒与胃肠道CaBP-D9k表达对不同日粮阴阳离子差的响应研究

doi:10.11843/j.issn.0366-6964.2014.06.011

Abstract

Abstract:

The study was conducted to clarify the mechanism of low dietary cation-anion difference (DCAD) level preventing hypocalcemia in transition cows by investigating the influence of DCAD levels on blood Ca concentration and calcium binding protein-9kDa (CaBP-D9k) expression level in gastrointestinal tract of transition goats.Twenty-seven Guizhou Black goats in transition period were randomly allocated to 3 treatments with 9 goats each treatment and were fed 3 diets with varying DCAD levels：high DCAD (HD，+325)，control (CON，+179) and low DCAD (LD，-110) groups，respectively.Goats were blooded on d 10 before lambing，d 0 for lambing and d 3 after lambing，respectively，and were slaughtered to detect the plasma parameters and CaBP-D9k expression level of gastrointestinal tract.Compared with high DCAD，feed intake was unaffected by low DCAD treatment (P>0.05)，urine pH of goats fed LD was reduced with decreasing DCAD level (P<0.05).Compared to CON and HD,reducing DCAD level induced higher plasma Cl^- and Ca²⁺ concentration of goats during the experiment (P<0.05).Greater CaBP-D9k mRNA expression level was observed in whole gastrointestinal tract of LD-fed goats except for ileum，cecum and rectum compared with goats in HD group (P<0.05).Moreover，feeding LD diet relieved the decline of CaBP-D9k mRNA expression level in abomasum，jejunum and colon after lambing (P<0.05).In conclusion，reducing DCAD could up-regulate CaBP-D9k expression level in stomach，proximal intestine and colon，and kept Ca²⁺ concentration for transition goats.The results indicated that Ca²⁺ absorption in the above gastrointestinal tracts was increased and would be the possible mechanism of low DCAD enhancing blood Ca homeostasis in dairy cows during periparturient period.

CLC Number:

S827
S816.32

YANG Yi，ZHANG Ji-kun，XIN Hai-liang，TIAN Xing-zhou，HAN Xiao-jie，DUAN Yong-bang，WANG Dai-lin，WU Wen-xuan，LI Sheng-li. Responses of Blood Calcium and Calcium Binding Proteins-D9k Expression Level to Dietary Cation-anion Difference of Transition Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(6): 932-942.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2014.06.011

https://www.xmsyxb.com/EN/Y2014/V45/I6/932

References

［1］USDA.Changes in the U.S.Dairy Cattle Industry，1991-2007 ［M］.USDA：APHIS：VS CEAH，National Animal Health Monitoring System，Fort Collins (CO)，2007：480.
［2］MARTIN-TERESO J，VERSTEGEN M W.A novel model to explain dietary factors affecting hypocalcaemia in dairy cattle ［J］.Nutr Res Rev，2011，24(2)：228-243.
［3］GRÜNBERG W，DOBBELAAR P，BREVES G.Kinetics of phosphate absorption in lactating dairy cows after enteral administration of sodium phosphate or calcium phosphate salts ［J］.Br J Nutr，2013，110(6)：1012-1023.
［4］WEICH W，BLOCK E，LITHERLAND N B.Extended negative dietary cation-anion difference feeding does not negatively affect postpartum performance of multiparous dairy cows ［J］.J Dairy Sci，2013，96(9)：5780-5792.
［5］ESPINO L，GUERRERO F，SUAREZ M L，et al.Long-term effects of dietary anion-cation balance on acid-base status and bone morphology in reproducing ewes ［J］.J Vet Med，2003，50(10)：488-495.
［6］LIESEGANG A.Influence of anionic salts on bone metabolism in periparturient dairy goats and sheep ［J］.J Dairy Sci，2008，91(6)：2449-2460.
［7］SPANGHERO M.Urinary pH and mineral excretion of cows fed four different forages supplemented with increasing levels of an anionic compound feed ［J］.Anim Feed Sci Technol，2002，98(3-4)：153-165.
［8］吴文旋，段永邦，李胜利.饲粮阴阳离子差对围产期奶牛酸碱平衡，血浆钙浓度及抗氧化应激的影响［J］.动物营养学报，2013，25(4)：856-863.
［9］李大刚.阴离子盐和维生素D对围产期奶牛钙调控机制和健康作用的研究［D］.北京：中国农业科学院，2006.
［10］GRÜNBERG W，DONKIN S S，CONSTABLE P D.Periparturient effects of feeding a low dietary cation-anion difference diet on acid-base，calcium，and phosphorus homeostasis and on intravenous glucose tolerance test in high-producing dairy cows ［J］.J Dairy Sci，2011，94(2)：727-745.
［11］THILSING-HANSEN T，JØRGENSEN R J，ØSTERGAARD S.Milk fever control principles：a review ［J］.Acta Veterinaria Scandinavica，2002，43(1)：1-19.
［12］杨艺，莘海亮，夏先林，等.昆明小鼠胃肠道钙离子跨膜吸收途径相关基因表达模式分析［J］.动物营养学报，2013，(2)：441-446.
［13］MARTIN-TERESO J，DISTEFANO C，VAN LAAR H，et al.Effect of feeding rumen-protected rice bran on mineral status of non-lactating dairy heifers ［J］.J Anim Physiol Anim Nutr，2011，95(1)：73-80.
［14］DEINER C，REICHE M，LASSNER D，et al.Allelic variations in coding regions of the vitamin D receptor gene in dairy cows and potential susceptibility to periparturient hypocalcaemia ［J］.J Dairy Res，2012，79(4)：423-428.
［15］CHOI K C，JEUNG E B.Molecular mechanism of regulation of the calcium-binding protein calbindin-D9k,and its physiological role(s) in mammals：a review of current research ［J］.J Cell Mol Med，2008，12(2)：409-420.
［16］LEE G S，LEE K Y，CHOI K C，et al.Phenotype of a calbindin-D9k gene knockout is compensated for by the induction of other calcium transporter genes in a mouse model ［J］. J Bone Mineral Res，2007，22(12)：1968-1978.
［17］WU Y，MIYAMOTO T，LI K，et al.Decreased expression of the epithelial Ca2+ channel TRPV5 and TRPV6 in human renal cell carcinoma associated with vitamin D receptor ［J］.J Urol，2011，186(6)：2419-2425.
［18］OLIVEIRA A G，AQUINO D J，MAHECHA G A，et al.Involvement of the transepithelial calcium transport disruption and the formation of epididymal stones in roosters ［J］.Reproduction，2012，143(6)：835-844.
［19］OTSUKA H，SUGIMOTO M，IKEDA S，et al.Effects of bisphenol A administration to pregnant mice on serum Ca and intestinal Ca absorption ［J］.Anim Sci J，2012，83(3)：232-237.
［20］REPLOGLE R A，LI Q，WANG L，et al.Gene-by-diet interactions influence calcium absorption and bone density in mice ［J］.J Bone Miner Res，2014，29(3)：657-665.
［21］GRüNBERG W，CONSTABLE P，SCHRÖDER U，et al.Phosphorus homeostasis in dairy cows with abomasal displacement or abomasal volvulus ［J］.J Vet Int Med，2005，19(6)：894-898.
［22］LEONHARD-MAREK S，STUMPFF F，MARTENS H.Transport of cations and anions across forestomach epithelia：conclusions from in vitro studies ［J］.Animal，2010，4(7)：1037-1056.
［23］WU W X，YANG Y，ZHANG J K，et al.Reducing dietary cation-anion difference on acid-base balance，plasma minerals level and anti-oxidative stress of female goats ［J］.J Integrative Agric，2013，12(9)：1620-1628.
［24］WU W X，LIU J X，XU G Z，et al.Calcium homeostasis，acid-base balance，and health status in periparturient Holstein cows fed diets with low cation-anion difference ［J］.Livest Sci，2008，117(1)：7-14.
［25］莘海亮，吴文旋，李胜利.动物胃肠道钙吸收关键基因表达实时荧光定量PCR检测方法的建立［J］.江苏农业科学，2013，(4)：31-34.
［26］YAMAGISHI N，YUKAWA Y A，ISHIGURO N，et al.Expression of calbindin-D9k messenger ribonucleic acid in the gastrointestinal tract of dairy cattle ［J］. J Vet Med Series A，2002，49：461-465.
［27］WANG Y M，WANG J H，WANG C，et al.Effect of different rumen-inert fatty acids supplemented with a dietary antioxidant on performance and antioxidative status of early-lactation cows ［J］.J Dairy Sci，2010，93(8)：3738-3745.
［28］陈波，刘建新.奶牛日粮阴阳离子差对采食量影响的Meta分析［J］.动物营养学报，2010，(1)：46-50.
［29］CHARBONNEAU E，PELLERIN D，OETZEL G R.Impact of lowering dietary cation-anion difference in nonlactating dairy cows：A meta-analysis ［J］. J Dairy Sci，2006，89(2)：537-548.
［30］MELENDEZ P，DONOVAN A，RISCO C A，et al.Metabolic responses of transition Holstein cows fed anionic salts and supplemented at calving with calcium and energy ［J］. J Dairy Sci，2002，85(5)：1085-1092.
［31］吴文旋.饲粮阴阳离子差与奶牛体液酸碱平衡的关联性研究［J］.动物营养学报，2011，23(12)：2198-2202.
［32］吴文旋.日粮阴阳离子差在奶牛上的应用效果研究［D］.杭州：浙江大学，2007.
［33］PENNER G B，TREMBLAY G F，DOW T，et al.Timothy hay with a low dietary cation-anion difference improves calcium homeostasis in periparturient Holstein cows ［J］.J Dairy Sci，2008，91(5)：1959-1968.
［34］HERON V S，TREMBLAY G F，OBA M.Timothy hays differing in dietary cation-anion difference affect the capability of dairy cows to maintain their calcium homeostasis ［J］.J Dairy Sci，2009，92(1)：238-246.
［35］STEWART P A.Modern quantitative acid-base chemistry ［J］.Can J Physiol Pharmacol，1983，61(12)：1444-1461.
［36］BERNE R M，LEVY M N，KOEPPEN B M，et al.Berne & Levy Physiology ［M］.Mosby/Elsevier，2008.
［37］National Research Council.Subcommittee on dairy cattle N.nutrient requirements of dairy cattle ［M］.National Academies Press，2001.
［38］ROCHE J R，PETCH S，KAY J K.Manipulating the dietary cation-anion difference via drenching to early-lactation dairy cows grazing pasture ［J］.J Dairy Sci，2005，88(1)：264-276.
［39］ROCHE J R，DALLEY D，MOATE P，et al.Dietary cation-anion difference and the health and production of pasture-fed dairy cows.1.Dairy cows in early lactation ［J］.J Dairy Sci，2003，86(3)：970-978.
［40］APPER-BOSSARD E，PEYRAUD J L，FAVERDIN P，et al.Changing dietary cation-anion difference for dairy cows fed with two contrasting levels of concentrate in diets ［J］.J Dairy Sci，2006，89(2)：749-760.
［41］CHRISTAKOS S.Recent advances in our understanding of 1,25-dihydroxyvitamin D(3) regulation of intestinal calcium absorption ［J］.Arch Biochem Biophys，2012，523(1)：73-76.
［42］WILKENS M R，OBERHEIDE I，SCHRÖDER B，et al.Influence of the combination of 25-hydroxyvitamin D3 and a diet negative in cation-anion difference on peripartal calcium homeostasis of dairy cows ［J］.J Dairy Sci，2012，95(1)：151-164.
［43］THILSING-HANSEN T，JORGENSEN R J.Hot topic：prevention of parturient paresis and subclinical hypocalcemia in dairy cows by zeolite A administration in the dry period ［J］.J Dairy Sci，2001，84(3)：691-693.
［44］HORST R L，GOFF J P，REINHARDT T A，et al.Strategies for preventing milk fever in dairy cattle ［J］.J Dairy Sci，1997，80(7)：1269-1280.
［45］DEGARIS P J，LEAN I J.Milk fever in dairy cows：a review of pathophysiology and control principles ［J］.Vet J，2008，176(1)：58-69.
［46］SHARIF M，SHAHZAD M A，MAHR UN N，et al.Influence of varying levels of dietary cation anion difference on ruminal characteristics，nitrogen metabolism and in situ digestion kinetics in buffalo bulls ［J］.Anim Sci J，2010，81(6)：657-665.
［47］吴文旋，李胜利.不同日粮阴阳离子差对喀斯特地区围产期奶牛代谢、健康及泌乳性能的研究［J］.畜牧兽医学报，2011，42(8)：1113-1119.
［48］MARTíN-TERESO LPEZ J.Rumen-protected rice bran to induce the adaptation of calcium metabolism in dairy cows ［D］.Wageningen：Wageningen University，2010.
［49］LIESEGANG A，SINGER K，BOOS A.Vitamin D receptor amounts across different segments of the gastrointestinal tract in Brown Swiss and Holstein Frisean cows of different age ［J］.J Anim Physiol Anim Nutr, 2008，92(3)：316-323.
［50］SIDLER-LAUFF K，BOOS A，KRAENZLIN M，et al.Influence of different calcium supplies and a single vitamin D injection on vitamin D receptor and calbindin D9k immunoreactivities in the gastrointestinal tract of goat kids ［J］.J Anim Sci, 2010，88(11)：3598-3610.
［51］WILKENS M R，RICHTER J，FRASER D R，et al.In contrast to sheep，goats adapt to dietary calcium restriction by increasing intestinal absorption of calcium ［J］.Comp Biochem Physiol-Part A：Mol Integ Physiol，2012，163(3-4)：396-406.
［52］WILKENS M R，MROCHEN N，BREVES G，et al.Gastrointestinal calcium absorption in sheep is mostly insensitive to an alimentary induced challenge of calcium homeostasis ［J］.Comp Biochem Physiol-Part B：Biochem Mol Biol，2011，158(3)：199-207.
［53］MUSCHER A S，WILKENS M R，MROCHEN N，et al.Ex vivo intestinal studies on calcium and phosphate transport in growing goats fed a reduced nitrogen diet ［J］. Br J Nutr，2012，108(4)：628-637.

Responses of Blood Calcium and Calcium Binding Proteins-D9k Expression Level to Dietary Cation-anion Difference of Transition Goats

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 0

Recommended Articles

Metrics

Comments