不同蛋白质饲料对宣汉黄牛瘤胃固相粘附蛋白分解菌数量的影响

doi:10.11843/j.issn.0366-6964.2014.06.013

Abstract

Abstract:

The objective of this paper was to investigate the effects of three protein feeds (soybean meal，rapeseed meal and cottonseed meal) on quantities of ruminal proteobacteria (R.amylophilus，B.fibrisolvens,P.ruminicoal and S.bovis) adhered to solid fractions in Sichuan Xuanhan Yellow cattle.Four rumen-cannulated Xuanhan Yellow cattle with similar healthy and weight were each fed four diets with different protein feeds over a period in a 4×4 Latin Square design，which were basal diet(CN)，replaced 15% concentration of basal diet with soybean meal (SBM)，rapeseed meal (RSM) and cottonseed meal (CSM)，respectively.The PCR technique was used to analyze the change of the population of ruminal proteobacteria adhensive to solid fractions.The results showed that: 1)the quantities of R.amylophilus，B.fibrisolvens，P.ruminicoal and S.Bovis in group SBM reached the highest point at 9，9，5 and 5 h after morning feeding，the group RSM at 3，7，7 and 1 h，the group CSM at 9，9，7 and 1 h.2)the highest mean value was found in group SBM while the lowest in the group CSM on R.amylophilus(P<0.05) quantity; the highest mean value was recorded in group CSM while the lowest in SBM on the quantities of B.fibrisolvens，P.ruminicoal and S.Bovis (P<0.05) quantities.In conclusion，different protein feeds significantly affected the quantities of ruminal proteobacteria，and cottonseed meal had a better promoting effect on ruminal proteobacteria adhensive to solid fractions.

CLC Number:

S823
S816.4

WU Xiao-yan，WANG Zhi-sheng，ZOU Hua-wei. Different Protein Sources Dietary Affect the Quantities of Rumen Proteobacteria Adhensive to Solid Fractions in Xuanhan Yellow Cattle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(6): 953-959.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2014.06.013

https://www.xmsyxb.com/EN/Y2014/V45/I6/953

References

［1］袁涛，张伟力.我国几种蛋白质饲料资源现状［J］.江西饲料，2004，(2):25-28.
［2］ATTWOOD G T，REILLY K.Identification of proteolytic rumen bacteria isolated from New Zealand cattle［J］.J Appl Bacteriol，1995,79：22-29.
［3］FERNANDO S C，PURVIS II H T，NAJAR F Z，et al.Rumen microbial population dynamics during adaptation to a high-grain diet［J］.Appl Environ Microbiol，2010,76(22)：7482-7490.
［4］KIM M，MORRISON M，YU Z，et al.Phylogenetic diversity of bacterial communities in bovine rumen as affected by diets and microenvironments［J］.Folia Microbiol，2011，56(5)：453-458.
［5］PETR R M，FORSTER R J，YANG W，et al.Characterization of rumen bacterial diversity and fermentation parameters in concentrate fed cattle with and without forage［J］.J Appl Microbiol,2012，112(6)：1152-1162.
［6］CARBERRY C A，KENNY，HAN S，et al.Effect of phenotypic residual feed intake and dietary forage content on the rumen microbial community of beef cattle［J］.Appl Environ Microbiol，2012，78(14)：4949-4958.
［7］冯薇,王加启,刘开朗，等.运PCR-DGGE分析比较瘤胃中不同饲料固相粘附微生物区系［J］.畜牧兽医学报，2010，41(12)：1556-1562.
［8］HUWS S A，LEE M R，MUETZEL S M，et al.Forage type and fish oil cause shifts in rumen bacterial diversity［J］.FEMS Microbiol Ecol，2010，73(2)：396-407.
［9］KONG Y，TEATHER R，FORSTER R.Composition，spatial distribution，and diversity of the bacterial communities in the rumen of cows fed different forages［J］.FEMS Microbiol Ecol，2010，74(3)：612-622.
［10］MATSUI H，USHIDA K，KOJIMA Y.Effect of dietary concentrate on fungal zoosporogenesis in sheep rumen［J］.Asia-Austr J Anim Sci,1997，10(6):599-602
［11］TAJIMA K，AMINOV R I，NAGAMINE T，et al.Diet-dependent shifts in the bacterial population of the rumen revealed with real-time PCR［J］.Appl Environ Microbiol,2001，67(6)：2766-2774.
［12］MCALLISTER T A，BAE H D，JONES G A,et al.Microbial attachment and feed digestion in the rumen［J］.J Anim Sci,1994,72:3004-3018.
［13］KIM M，MORRISON M，YU Z.Status of the phylogenetic diversity census of ruminal microbiomes［J］.FEMS Microbiol Ecol，2011,76:49-63.
［14］KANG S，DENMAN S E，MORRISON M I.An efficient RNA extraction method for estimating gut microbial diversity by polymerase chain reaction［J］.Current Microbiol,2009,58:464-471.
［15］LAURE R，YU Z T，PARISI V A.Novel microbial diversity adherent to plant biomass in the herbivore gastrointestinal tract，as revealed by ribosomal intergenic spacer analysis and rrs gene sequencing［J］.Environ Microbiol,2005，(7)：530-543.
［16］MUYEZER Y G，DEWAAL E C，UITTERLINDEN A G.Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase china reaction amplified genes encoding for 16S rRNA［J］. Appl Environ Microbiol,1993，59：695-700.
［17］徐爱秋,王梦芝,王洪荣，等.体外培养条件下氨基酸对瘤胃蛋白质降解菌生长限制性的影响［J］.动物营养学报,2010,22(1):88-92.
［18］KHAFIPOUR E,PLAIZIER J C.Rumen microbiome composition determined using two nutritional models of subacuteruminal acidosis［J］.Appl Environ Microbiol,2009,75(22):7115-7124．
［19］WANG M Z，WANG H R，CAO H C，et al.Effects of limiting amino acids on rumen fermentation and microbial community in vitro［J］.Agricultural Sciences in China，2008,7(12):1524-1531.
［20］RUSSELL J B，OCONNOR J D，FOX D G.Net carbohydrate and protein system for evaluating cattle diets：Ⅰ.Ruminal fermentation［J］.J Anim Sci,1992,70(11):3551-3561.
［21］DEVANT M，FERRET A，CALSAMIGLIA S，et al.Effect of nitrogen source in high-concentrate low-protein beef cattle diets on microbial fermentation studied in vivo and in vitro［J］.J Anim Sci，2001，79:1944-1953.
［22］YAN T，AGNEW R E.Prediction of nutritive values in grass silages：Ⅰ.Nutrient digestibility and energy concentration using nutrient compositions and fermentation characteristics［J］.J Anim Sci，2004,82:1367-1379.
［23］HETTA M，CONE J W，GUSTAVSSON A M，et al.The effect of additives in silages of pure timothy and timothy mixed with red clover on chemical composition and in vitro rumen fermentation characteristics［J］. Grass Forage Sci，2003，58:249-257.
［24］王梦芝,喻礼怀,王洪荣，等.不同蛋白质饲料对瘤胃微生物体外发酵和群体结构的影响［J］.动物营养学报,2009,21(5):673-679.
［25］王梦芝,沈建昭,刘莹，等.蛋白质来源对瘤胃细菌和原虫群体结构的影响［J］.中国畜牧杂志,2011,47(3):35-40.
［26］许曾曾.蛋白质饲料种类和添加水平对活体外瘤胃发酵和微生物氨基酸组成的影响［D］.北京:中国农业大学,2004.
［27］GHOORCHI T，ARBABI S.Study of protein characteristic of five feeds by CNCPS mIodel［J］.Asian J Anim Vet Adv，2010,5(8):584-591.
［28］周荣,王加启,潘发明，等.饲料蛋白组分对反刍家畜常用饲料小肠吸收蛋白质含量的影响［J］.华北农学报，2010,25(B12):165-169.
［29］何英.糠麸,糟渣,饼粕类饲料猪有效能预测模型的研究［D］.雅安:四川农业大学,2004.
［30］BLACKBURN T H.Production by Bacteroides amylophilus strain H18［J］. J General Microbiol，1968，53：27-36.

Different Protein Sources Dietary Affect the Quantities of Rumen Proteobacteria Adhensive to Solid Fractions in Xuanhan Yellow Cattle

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 1

Recommended Articles

Metrics

Comments