奶牛瘤胃中1株反-11-油酸氢化菌的筛选及鉴定

doi:10.11843/j.issn.0366-6964.2014.01.010

Abstract

Abstract:

The purpose of this study was to identify trans-vaccenic acid (trans-11 C18:1，t-VA) hydrogenating bacteria in vitro from rumen of dairy cows and to analyze biochemistry characteristics and phylogenetic position.The t-VA-hydrogenating bacterium was isolated from the rumen of Holstein dairy cows based on enrichment culture under anaerobic conditions.The strains with high degree of hydrogenation were screened.The biochemical map were detected by gas chromatograph and the biochemical characterization of RV was analyzed by VITEK 2 compact system.The 16S rDNA，sodA，rpoB and recN genes were sequenced and used for phylogenic tree building.The newly isolate bacteria named RV with t-VA hydrogenating efficiency of 82.1% was isolated.RV was strictly anaerobic，gram-staining-positive，globose or oval shaped，and had optimum growth performance at 39 ℃and pH 6.0-7.8.The carbon or energy sources of RV came from D-xylose，D-galactose，D-maltose，D-mannose，pullulan，sacchrose，D-trehalose，D-cellobiose，etc.It contained some enzyme activities (e.g alpha-galactosidase and arylamidase) and resistant phylotpye to bacitracin，novobiocin，polymixin B.The gram-positive biochemical map showed that the similarity of RV reached 99% to that of pig intestine Streptococcus.The sequence of 16S rDNA of RV had high identity to Streptococcus equinus and Streptococcus bovis (99%-100%).The sequence of the funtional genes(sodA，rpoB and recN) had the hightest homology to S.bovis(99%，99% and 98%).One t-VA hydrogenating bacteria (a strain from S.bovis) named RV is isolated from rumen.To our known，this is the first study to find that S.bovis has t-VA hydrogenating activity.

CLC Number:

S823
S815.4

LU Yu-fei，WANG Jia-qi，ZHAO Sheng-guo，BU Deng-pan，ZHOU Ling-yun，SUN Peng. Screening and Characterization of trans-11 18:1 Hydrogenating Bacteria from Rumen of Dairy Cows[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(1): 69-77.

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Screening and Characterization of trans-11 18:1 Hydrogenating Bacteria from Rumen of Dairy Cows

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