ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (10): 1875-1881.doi: 10.11843/j.issn.0366-6964.2015.10.022

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Dandelion Aqueous Extract Affects Biofilm Formation by S.suis

YU Wen-hui1,XU Jing1,WEI Qing-wei1,JIANG Xiao-wen1,SHAN An-shan2*   

  1. (1.College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China;2.College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China)
  • Received:2015-02-02 Online:2015-10-23 Published:2015-10-23

Abstract:

The objective of this study was to investigate whether or not dandelion aqueous extract combated bacterial growth or affected biofilm formation by S.suis.In this study,the chlorogenic acid content of the dandelion aqueous extract was detected by high efficiency liquid chromatography (HPLC).Using a microdilution broth method,the MIC or MBC of dandelion aqueous extract or chlorogenic acid against S.suis was determined.The S.suis biofilm was determined by crystal violet staining.The S.suis biofilm was also examined by scanning electron microscopy.The results revealed that the chlorogenic acid content of the dandelion aqueous extract was 5.833 mg•mL-1.The MIC,MBC of dandelion aqueous extract to S.suis was 62.5,250 mg•mL-1,respectively.The MIC,MBC of chlorogenic acid to S.suis was 2.5,10 mg•mL-1,respectively.In the negative control,scanning electron microscopy analysis revealed that bacteria were embedded within the biofilms,and the thick slimes revolved around the bacteria.However,the biofilms were significantly affected following growth in the presence of dandelion aqueous extract or chlorogenic acid.Moreover,biofilm formation by S.suis was dose-dependently decreased by sub-MICs of dandelion aqueous extract or chlorogenic acid.Sub-MICs of dandelion aqueous extract or chlorogenic acid also diminished bacterial population by S.suis.There were no slimes and extracellular matrix components in sub-MICs of dandelion aqueous extract or chlorogenic acid treated s.suis biofilm.In summary,our study showed that sub-MICs of taraxaci herba aqueous extract significantly decreased biofilm formation by S.suis,and one of the active ingredients in dandelion aqueous extract was chlorogenic acid.

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