ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2013, Vol. 44 ›› Issue (7): 1037-1042.doi: 10.11843/j.issn.0366-6964.2013.07.007

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The Effect of Melatonin on the Oxidative Stress of Oocyte and Parthenogenetic Development in Mouse

JIANG Yuan-yuan，ZHANG Bao-xiu，SUN Jing-tao，LI Zhong-shu，FANG Nan-zhu*   

  1. (Laboratory of Animal Genetic Breeding and Reproduction，Agricultural College of Yanbian University，Yanji 133002，China)
  • Received:2012-12-24 Online:2013-07-23 Published:2013-07-23

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Abstract:

 Mouse oocyte were cultured in maturation medium to investigate melatonin(MT)could affect mouse oocyte maturation during exogenous H2O2.the parthenogenetic embryos were incubated with different concentrations of MT(0,10-9,10-7,10-5,10-3 mol·L-1), every stage of development would be analyzed. The results showed：(1) The percentage of the mature oocytes (MⅡstage oocytes with a first polar body) was significantly decreased by the addition of exogenous H2O2 in a dose-dependent manner (>250 μmol·L-1, 19.4% vs.92.0%, P<0.05). (2)When oocytes were incubated with MT（0.1，1.0，10.0 ng·mL-1） in the presence of H2O2 (250 μmol·L-1), MT dose-dependently blocked the inhibitory effect of H2O2 on oocyte maturation, there was a significant effect at the concentration of 10.0 ng·mL-1 of MT. (3)GV stage oocytes were incubated with 2′,7′-dichlorofluorescein (DCF-DA) after co-culture of H2O2(500 μmol·L-1) and MT（0.000，0.001，0.010，0.100 ng·mL-1） ,the increased fluorescence intensity of oocytes incubated with H2O2 was significantly decreased by MT treatment(0.100 ng·mL-1）.(4) There was no significant difference in the groups of 10-9-10-3 mol·L-1 than the control group during two-cell phase (88.2%, 91.4%, 91.8%, 88.8% vs. 88.1%, P>0.05), the rate of four-eight-cell was improved when added 10-9M of MT (30.5% vs. 23.4%, P>0.05). There was very significant difference among the rates of 10-7-10-3 mol·L-1 than control group(58.8%,73.8%,43.6% vs.23.4%, P<0.05), Blastocyst rates were increased in the higher concentrations 10-9-10-5 mol·L-1 (24.6%,46.6%,49.2% vs.8.0%, P<0.05),but it decreased during 10-3 mol·L-1 of MT. Oxidative stress was harmful for oocytes maturation which were protected by MT. The optimum concentration of MT for development of mouse oocytes after parthenogenetic activation was 10-5 mol·L-1. As a direct free radical scavenger, MT could become one of the most important conditions for improving oocyte and embryo quality.

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