Abstract: The experiment was conducted to determine the effect of exogenous glutamine on the growth performance, carcass traits, meat quality, the content of inosine monophosphate and the expression level of H-FABP in breast and thigh muscle of AA male broilers. A total of 240 AA male broilers were randomly divided into 4 groups (A, B, C and D) which were fed with basic diet supplemented with Glu at 0, 0.2%, 0.4% and 0.8% level, respectively, the average daily gain(ADG), average daily feed intake(ADFI) and feed/gain ratio(F/G) were determined at 21^st and 42^nd day, and the experiment lasted for 42 days. The shear force, drip loss and chromal(L^*, a^* and b^*) were determined and the dressing percentage, eviscerated yield, half eviscerated yield, percentage of breast and thigh muscle were calculated at 42^nd day. The content of inosine monophosphate was detected by HPLC, and RT-PCR was used to determine the expression level of H-FABP mRNA in breast and thigh muscle. The average daily gain, average daily intake, the content of inosine monophosphate and the expression level of H-FABP mRNA of broilers were improved significantly in group B(P<0.05). The eviscerated yield, percentage of thigh muscle, the breadth of subcutaneous fat, shear force of breast were increased and percentage of abdominal fat were decreased significantly in group C (P<0.05). The shear force of thigh, the L^* and a^* value of breast and thigh muscle were enhanced significantly in group D (P<0.05). The result indicate that Glu supplement at 0.2%, 0.4% and 0.8% levels could improve the growth performance, carcass traits and meat quality of broilers effectively, and the contents of inosine monophosphate and expression level of H-FABP mRNA in breast and thigh muscle could be increased to some extent. The 0.2% level is the recommended dosage in the production.