Abstract:

This experiment was conducted to study the influence of Lactobacillus acidophilus (LAB1) on the focal adhesion kinase (FAK) phosphorylation and tight junction protein occludin expression. IPEC-J2 cells were infected by three concentrations of pathogenic Escherichia coli (E. coli) and Salmonella choleraesuis (S. cho), and then treated with LAB1. Occludin protein level was detected by ELISA, and FAK(Y³⁹⁷) phosphorylation was detected by Western blotting. Results were as follows: In LAB1 treated S. cho test, Occludin protein and FAK phosphorylation levels were positive correlated with the LAB1 concentration, but were negative correlated with S. cho concentration, and the differences were significant among groups that treated by mid and low concentrations of S. cho (P<0.05); In LAB1 treated E. coli test, Occludin protein and the level of FAK phosphorylation were positively correlated with LAB1 concentration, and the differences were significant among groups that treated by low concentrations of E. coli (P<0.05). Conclusion: LAB1 can activate the phosphorylation of FAK which inhibited by E. coli and S. cho, and raise regulate the cells occludin expression.