负载重组口蹄疫病毒VP4蛋白的树突状细胞启动的T细胞应答

Abstract

Abstract: This study was designed to seek the activation effect on T lymphocytes by dendritic cells pulsed with recombinant VP4 protein of foot-and-mouth disease virus (FMDV). To this end, we have successfully constructed the prokaryotic expression vector of pET32a-VP4. The recombinant VP4 protein was expressed with induction of IPTG. VP4 protein was purified by SDS-PAGE and electro-elution approaches and identified with Western blot. Bone morrow-derived dendritic cells (BMDCs) were generated in vitro and pulsed with purified VP4 protein. The VP4-loaded BMDCs were co-cultured with lymph node T cells. Culture supernatants were harvested at indicated time points and the levels of IFN-γ were assayed with ELISA. The results showed that the IFN-γ levels of test groups were higher than that of control groups significantly at 3, 9, and 48 hours after co-culture. These data indicate that BMDCs pulsed with FMDV VP4 protein are able to efficiently stimulate T lymphocyte activation and consequently initiate Th1-like response with enhanced secretion of IFN-γ.

CLC Number:

S852.659.6

AN Peng-li, LI Na, LI Li-min, ZHANG Li, ZHANG Lei, GAO Yun-huan, WANG Jia-xin. T Cell-mediated Responses Initiated by Dendritic Cells Pulsed with Recombinant VP4 Protein of Foot-and-mouth Disease Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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T Cell-mediated Responses Initiated by Dendritic Cells Pulsed with Recombinant VP4 Protein of Foot-and-mouth Disease Virus

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