鸡前脂肪细胞中A-FABP基因表达的变化对PPARγ、perilipin和E-FABP表达的影响

Abstract

Abstract: This study was designed to investigate the role of chicken A-FABP gene in lipid metabolism of adipocytes and the regulation relationship between A-FABP gene and the genes related to lipid metabolism. The shRNA interference vector with A-FABP gene and eukaryotic expression vector were used. Chicken adipocytes with short hairpin RNA-mediated knockdown and A-FABP cDNA overexpression were used. The genes expression level in chicken adipocytes were detected at 24, 36, 48, 60 and 72 h after transfection with the interference and overexpression plasmids of A-FABP. The results showed that the expression levels of PPARγ and perilipin genes were downregulated at 24 and 36 h and upregulated at 48 h in the A-FABP interference group. The expression levels of E-FABP genes was downregulated at 48 and 72 h in the A-FABP interference group. The expression levels of PPARγ and perilipin genes were upregulated at 24 and 36 h and downregulated at 48 h in the A-FABP overexpression group. The expression levels of E-FABP genes was downregulated at all detected time pionts in the A-FABP overexpression group. These results indicated that A-FABP might affect expression of PPARγ, perilipin and E-FABP in chicken adipocytes.

CLC Number:

S831.2

ZHANG Qing-qiu, SHI Hui, WANG Yu-xiang, LI Hui,WANG Qi-gui. The Effect of A-FABP Gene Overexpression or Knockdown on the Expression of PPARγ, perilipin and E-FABP in Chicken Adipocytes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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The Effect of A-FABP Gene Overexpression or Knockdown on the Expression of PPARγ, perilipin and E-FABP in Chicken Adipocytes

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