Abstract: To construct human adenovirus type 5 vectored recombinant virus expressing S1 glycoprotein gene of avian infectious bronchitis virus (IBV), S1 gene of IBV QS10 strain obtained through RT-PCR was inserted into shuttle plasmid of the adenoviral expression system, forming pacAd5 CMV-S1, as well as the backbone plasmid, which was linearized with PacⅠand transfected into 293AD cells. Detection with PCR revealed that the recombinant virus was successfully packaged with a titer up to 10⁷ TCID₅₀·mL^－1. Furthermore, The results of Western blotting and indirect immunofluorescence assay (IFA) indicated that the S1 gene was significantly expressed. Sera antibodies against IBV of the SPF chicken immunized with the recombinant virus were detected by enzyme-linked immunosorbent assay (ELISA). The recombinant adenovirus expressing S1 gene of IBV was successfully constructed with potent capacity to induce the specific antibodies in chickens and had the good immunogenicity, which laid the foundation for further development of recombinant avian infectious bronchitis vaccine.